Pathways in the brain, heart and lung influenced by SARS-CoV-2 NSP6 and SARS-CoV-2 regulated miRNAs: anin silicostudy hinting cancer incidence DOI Creative Commons
Shrabonti Chatterjee, J. Mahata, Suneel Kateriya

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 12, 2024

Abstract The influence of SARS-CoV-2 non-structural protein in the host’s tissue-specific complexities remains a mystery and needs more in-depth attention because COVID-19 recurrence long COVID. Here we investigated transmembrane NSP6 (Non-structural 6) three major organs - brain, heart, lung silico. To elucidate interplay between host proteins, analyzed protein-protein interaction network proteins interacting with proteins. Reported partners were ATP5MG, ATP6AP1, ATP13A3, SIGMAR1. Pathway enrichment analyses provided global insights into biological pathways governed by differentially regulated genes tissues after infection. Hub interactome analysed for drug targets many found. miRNA-gene was sought. Comparing this list gene targetted miRNAs, found two common brain respectively. Among five revealed as potential therapeutic across tissues, four non-approved drugs one approved could target Galectin 3 (LGALS3) AIFM1 Increased expression LGALS3 (that upregulated heart infection) is observed multiple cancers acts modulator tumor progression. infection also causes myocardial inflammation failure (HF). HF to be increasing cancer incidence. present scenario recurrent infections warrants studies probe effect on increased Graphical

Language: Английский

Oxidative Damage and Post-COVID Syndrome: A Cross-Sectional Study in a Cohort of Italian Workers DOI Open Access
Angela Stufano, Camilla Isgrò, Luigi Leonardo Palese

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7445 - 7445

Published: April 18, 2023

In addition to the acute symptoms after infection, patients and society are also being challenged by long-term effects of COVID-19, known as long COVID. Oxidative stress, a pivotal point in pathophysiology could potentially be involved development post-COVID syndrome. The aim present study was evaluate relationship between changes oxidative status persistence long-COVID workers with previous mild COVID-19 infection. A cross-sectional conducted among 127 employees an Italian university (80 47 healthy subjects). TBARS assay used detect malondialdehyde serum levels (MDA), while total hydroperoxide (TH) production measured d-ROMs kit. significant difference mean MDA values found previously infected subjects controls (4.9 µm vs. 2.8 µm, respectively). Receiver–operating characteristic (ROC) curves showed high specificity good sensibility (78.7% 67.5%, respectively) for levels. random forest classifier identified hematocrit value, levels, IgG titer against SARS-CoV-2 features highest predictive value distinguishing 34 from 46 asymptomatic subjects. damage persists suggesting possible role stress mediators pathogenesis

Language: Английский

Citations

34
Non-cross-reactive epitopes dominate the humoral immune response to COVID-19 vaccination – kinetics of plasma antibodies, plasmablasts and memory B cells DOI Creative Commons
Kilian A. Wietschel,

Kevin Fechtner,

Elmer Antileo

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: May 14, 2024

COVID-19 vaccines are highly effective in inducing protective immunity. While the serum antibody response to vaccination has been studied depth, our knowledge of underlying plasmablast and memory B cell (Bmem) responses is still incomplete. Here, we determined a naïve population contrasted it with single influenza primed cohort. In addition, analyzed against four endemic human coronaviruses (HCoVs).

Language: Английский

Citations

6
Neurological manifestations of SARS-CoV-2 infection in children in Taiwan: A cross-section, multicenter study DOI Creative Commons
Yen-Ju Chu,

Lee‐Chin Wong,

Che-Sheng Ho

et al.

Journal of the Formosan Medical Association, Journal Year: 2024, Volume and Issue: 123(7), P. 811 - 817

Published: Feb. 14, 2024

The SARS-CoV-2 virus has been a global public health threat since December 2019. This study aims to investigate the neurological characteristics and risk factors of coronavirus disease 2019 (COVID-19) in Taiwanese children, using data from collaborative registry. A retrospective, cross-sectional, multi-center was done an online network pediatric COVID-19 cohort total 11160 COVID-19-associated emergency department (ED) visits 1079 hospitalizations were analyzed. Seizures most common specific symptom, while encephalitis acute disseminated encephalomyelitis (ADEM) prevalent severe involvement. In ED patients with manifestations, diagnosis associated visual hallucination, seizure with/without fever, behavior change, decreased GCS, myoclonic jerk, activity/fatigue, lethargy. hospitalized fatigue, hypoglycemia at admission. Encephalitis/ADEM only factor for poor outcomes discharge patients. Neurological complications are COVID-19. Visual seizure, admission important warning signs involvement such as encephalitis/ADEM.

Language: Английский

Citations

4
Basic implications on three pathways associated with SARS-CoV-2 DOI Creative Commons
Jong‐Hoon Lee, Consolato Sergi, Richard E. Kast

et al.

Biomedical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100766 - 100766

Published: July 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts between the host and virus govern induction, resulting in multiorgan impacts. Its pathophysiology involves followings: 1) angiotensin-converting enzyme (ACE2) Toll-like receptor (TLR) pathways: 2) neuropilin (NRP) pathway: 3) spike protein pathway. Therefore, it is necessary to block pathological course with modulating innate lymphoid cells against diverse corona variants future.

Language: Английский

Citations

4
Afobazole: a potential drug candidate which can inhibit SARS CoV-2 and mimicry of the human respiratory pacemaker protein DOI
Govinda Rao Dabburu, Anjali Garg, Neelja Singhal

et al.

In Silico Pharmacology, Journal Year: 2025, Volume and Issue: 13(1)

Published: Feb. 17, 2025

Language: Английский

Citations

0
Persistent IgG1 clones dominate and personalize the plasma antibody repertoire DOI Creative Commons
Danique M.H. van Rijswijck, Albert Bondt, Dina Raafat

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(16)

Published: April 16, 2025

Antibodies play a pivotal role in the immune defense and long-term immunity. Yet, while several studies have highlighted persistence of antigen-specific antibody responses, it is unclear whether this stems from continuous production same clones or recurrent activation B cells generating new clones. To examine stability human repertoire, we monitored concentrations most abundant IgG1 plasma samples 11 healthy donors at nine sampling points over year. During year, each donor received three doses COVID-19 vaccine. Notwithstanding these vaccinations, remained constant. Given 2- to 3-week half-life molecules blood, our data suggest that are associated with immunity do not undergo somatic hypermutation which would imply short-lived cells. Overall, persistently produced by long-lived

Language: Английский

Citations

0
Identifying Neurological Autoantibodies in COVID‐19: mGluR2 as a Marker of Immune Dysregulation During the Omicron Outbreak in China DOI
Ziyan Wu, Siyuan Fan,

Honglin Xu

et al.

Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(5)

Published: May 1, 2025

ABSTRACT Aimed to comprehensively investigate the presence of neural autoantibodies in cerebrospinal fluid (CSF) and plasma COVID‐19 patients experiencing neurological complications during Omicron wave China. Forty consecutive with severe 15 disease controls (DC) were enrolled. Neural detected using both indirect immunofluorescence assay (IFA) on mouse brain tissue Brain‐neuronal‐antigen microarray. Our results indicated a significantly higher prevalence CSF (62.16% vs. 0.0%) (38.71% 13.33%) compared DC. Additionally, we identified 12 upregulated intrathecal IgG differential levels between DC, as well 51 plasma. A high anti‐mGluR2 antibodies (13.33%) was confirmed by cell‐based assays. Western blot analysis showed these cross‐react nucleocapsid (N) spike (S) proteins SARS‐CoV‐2. Notably, strong binding S protein's RBD‐Fc mGluR2 observed, an association that substantiated bioinformatics evaluating similarity SARS‐CoV‐2 targeted antigens This finding hints at potential cross‐reactivity protein patients.

Language: Английский

Citations

0
The picture theory of seven pathways associated with COVID-19 in the real world DOI Creative Commons
Jong‐Hoon Lee, Consolato Sergi, Richard E. Kast

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 8, 2024

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces immune-mediated diseases. Interactions between the host and virus govern induction, resulting in multiorgan impacts. In 2021, as normal life was challenging during pandemic era, we analyzed SCI journals according to L. Wittgenstein's Tractatus Logi-co-Philosophicus. The pathophysiology of disease 2019 (COVID-19) involves following steps: 1) angiotensin-converting enzyme (ACE2) Toll-like receptor (TLR) pathways: 2) neuropilin (NRP) pathway, with seven papers continuing twenty-four: 3) sterile alpha motif (SAM) histidine-aspartate domain (HD)-containing protein 1 (SAMHD1) tetramerization two twelve: 4) inflammasome activation pathways, five thirteen: 5) cytosolic DNA sensor cyclic-GMP-AMP synthase (cGAS)/stimulator interferon genes (STING) (cGAS–STING) signaling six successful eleven: 6) spike fourteen twenty-three: 7) immunological memory engram thirteen successive eighteen: 8) excess acetylcholine three nine. We reconfirmed that COVID-19 (1-7) pathways a new pathway involving acetylcholine. Therefore, it is necessary therapeutically alleviate block pathological course harmoniously modulating innate lymphoid cells (ILCs) if diverse SARS-CoV-2 variants are subsequently encountered future.

Language: Английский

Citations

2
Impact of COVID-19 on the brain: Antibodies against brain stem antigens DOI
Guglielmo Lucchese, Antje Vogelgesang, Angela Stufano

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 385 - 391

Published: Jan. 1, 2024

Language: Английский

Citations

1
Aggravating mechanisms from COVID-19 DOI Creative Commons
Jong‐Hoon Lee, Consolato Sergi, Richard E. Kast

et al.

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 27, 2024

Language: Английский

Citations

1