Global profiling of protein lactylation in Caenorhabditis elegans DOI Creative Commons
Tao Ding, Yehong Yang, Qiaochu Wang

et al.

PROTEOMICS, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 17, 2023

Abstract Lactylation, as a novel posttranslational modification, is essential for studying the functions and regulation of proteins in physiological pathological processes, well gaining in‐depth knowledge on occurrence development many diseases, including tumors. However, few studies have examined protein lactylation one whole organism. Thus, we studied global Caenorhabditis elegans to obtain an vivo lactylome. Using MS‐based platform, identified 1836 Class I (localization probabilities > 0.75) lactylated sites 487 proteins. Bioinformatics analysis showed that were mainly located cytoplasm involved tricarboxylic acid cycle (TCA cycle) other metabolic pathways. Then, evaluated conservation different organisms. In total, 41 C. homologous humans rats. Moreover, H4K80 was conserved three species. An additional 238 first time. This study establishes lactylome database provides basis role lactylation.

Language: Английский

Dysbiosis and Metabolic Dysregulation of Salivary Microbiota in Schizophrenia DOI Creative Commons
Jie Wang, Lin Lü,

Luwen Ren

et al.

Journal of Multidisciplinary Healthcare, Journal Year: 2025, Volume and Issue: Volume 18, P. 813 - 825

Published: Feb. 1, 2025

Background: Schizophrenia (SZ) is a chronic, severe mental disorder that presents significant challenges to diagnosis and effective treatment. Emerging evidence suggests gut microbiota may play role in the disease's pathogenesis. However, fewer studies have directly investigated potential links between oral SZ. Purpose: This study aimed explore relationship salivary dysbiosis SZ, examining microbial metabolic alterations contribute SZ pathophysiology. Methods: Salivary samples from 30 hospitalized patients diagnosed with 10 healthy controls were collected. The profiles analyzed using 16S rRNA gene sequencing metabolomic profiling. Clinical parameters, including health status, also evaluated minimize variability sampling. Results: Patients exhibited significantly poorer compared controls, more missing teeth worse periodontal status. Microbiota revealed notable overall structure composition of microbiome patients, characterized by increased abundance specific genera such as Neisseria Porphyromonas . Metabolomic analysis indicated differences control groups, upregulation key pathways, "β-alanine metabolism" "vitamin digestion absorption". Correlations elevated levels certain metabolites, L-methionine sulfoxide (L-MetO) tyramine, observed, suggesting oxidative stress. Conclusion: highlights presence dysfunction These results provide new insights into diagnostic biomarkers therapeutic targets for Further larger sample sizes are required validate these findings. Keywords: schizophrenia, microbiome, dysbiosis, rRNA, metabolome

Language: Английский

Citations

0
Applications and prospects of phosphoproteomics in renal disease research DOI Creative Commons

Xuejia Zheng,

Lingling Zhou, Tiantian Xu

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18950 - e18950

Published: March 20, 2025

Introduction Phosphoproteomics, an advanced branch of molecular biology, utilizes specific techniques such as mass spectrometry, affinity chromatography, and bioinformatics analysis to explore protein phosphorylation, shedding light on the cellular mechanisms that drive various biological processes. This field has become instrumental in advancing our understanding renal diseases, from identifying underlying pinpointing new therapeutic targets. Areas covered review will discuss evolution phosphoproteomics its early experimental observations current application disease research using liquid chromatography-tandem spectrometry (LC-MS/MS). We role identification biomarkers, elucidation pathogenic mechanisms, development novel strategies. Additionally, potential enhancing drug discovery improving treatment outcomes for diseases be highlighted. Expert opinion Phosphoproteomics is rapidly transforming by offering unprecedented insights into Utilizing LC-MS/MS, it enables biomarkers targets, mechanisms. promises significant advancements diagnosis shifting towards more personalized effective As technology evolves, integration clinical practice pivotal revolutionizing healthcare.

Language: Английский

Citations

0
Prediction of SARS-CoV-2 Infection Phosphorylation Sites and Associations of these Modifications with Lung Cancer Development DOI
Wei Li, Gen Li, Yuzhi Sun

et al.

Current Gene Therapy, Journal Year: 2023, Volume and Issue: 24(3), P. 239 - 248

Published: Nov. 10, 2023

Introduction: Since the emergence of SARS-CoV-2 viruses, multiple mutant strains have been identified. Infection with virus leads to alterations in host cell phosphorylation signal, which systematically modulates immune response. Methods: Identification and analysis infection sites enable insight into mechanisms viral effects on cells, providing important fundamental data for study development potent drugs treatment inflammatory diseases. In this paper, we analyzed virus-infected region developed a transformer-based deep learning-assisted identification method specific cells. Results: Furthermore, through association lung cancer, found that may affect regulatory role system, leading an abnormal increase or decrease response, be associated progression cancer. Conclusion: We anticipate will provide reference evolution as well immune-related studies reliable complementary screening tool anti-SARS-CoV-2 drug vaccine design.

Language: Английский

Citations

7
Proteome and ubiquitinome analyses of the brain cortex in K18-hACE2 mice infected with SARS-CoV-2 DOI Creative Commons
Qiaochu Wang, Wanjun Peng, Yehong Yang

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(9), P. 110602 - 110602

Published: July 27, 2024

Clinical research indicates that SARS-CoV-2 infection is linked to several neurological consequences, and the virus still spreading despite availability of vaccinations antiviral medications. To determine how hosts respond infection, we employed LC-MS/MS perform ubiquitinome proteome analyses brain cortexes from K18-

Language: Английский

Citations

1
A review of the roles of pathogens in Alzheimer’s disease DOI Creative Commons
Meng Zhao, Yongchun Wang, Yanxin Shen

et al.

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Aug. 19, 2024

Alzheimer’s disease (AD) is one of the leading causes dementia and characterized by memory loss, mental behavioral abnormalities, impaired ability to perform daily activities. Even as a global that threatens human health, effective treatments slow progression AD have not been found, despite intensive research significant investment. In recent years, role infections in etiology has sparked intense debate. Pathogens invade central nervous system through damaged blood–brain barrier or nerve trunk disrupt neuronal structure function well homeostasis brain microenvironment series molecular biological events. this review, we summarize various pathogens involved pathology, discuss potential interactions between AD, provide an overview promising future anti-pathogenic therapies for AD.

Language: Английский

Citations

1
Characterization of the Pathogenic Features of Multiple SARS-CoV-2 Pandemic Strains in Different Mouse Models DOI

Huize Sun,

Kunpeng Liu,

Baocheng Yu

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Characterization of the Pathogenic Features of Multiple SARS‐CoV‐2 Pandemic Strains in Different Mouse Models DOI

Huize Sun,

Kunpeng Liu,

Baocheng Yu

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(11)

Published: Nov. 1, 2024

ABSTRACT Elucidating the detailed features of emerging SARS‐CoV‐2 strains both in vitro and vivo is indispensable for development effective vaccines or drugs against viral infection. We thoroughly characterized virological pathogenic eight different pandemic strains, from WT strain to current circulating sublineage EG.5.1, vivo. Besides observed Vero E6 cells, Omicron variants, BA.1 exhibited enhanced infectious effects upper respiratory tract K18 human angiotensin‐converting enzyme (ACE2) (K18 hACE2) transgenic mice. Both XBB.1.9.1 EG.5.1 presented stronger tropism brain, which could be main reason increased lethal on In addition, pathogenesis comparisons among all these viruses C57BL/6JGpt mice indicated that variant two new sublineages possessed dual tropisms mice, were further confirmed by subsequent bioinformatic analyses actual affinity comparison between RBDs mouse receptor ACE2. Furthermore, immunocompromised BKS‐db found more susceptible compared revealed infectivity was determined its host immunocompetence. Thus, this study not only contributes a systematic understanding but also provides insights combat potential future surges variants.

Language: Английский

Citations

0
Global Profiling of Protein Lactylation in Human Hippocampi DOI

Chun‐Mei Shi,

Qiaochu Wang, Xiaolu Li

et al.

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

The hippocampus has long been associated with cognition and memory function, the implications of lysine lactylation (Kla), a recently identified post-translational modification (PTM), in role remain largely unexplored.

Language: Английский

Citations

0
FrozONE: quick cell nucleus enrichment for comprehensive proteomics analysis of frozen tissues DOI Creative Commons

Lukas A Huschet,

Fabian P. Kliem,

P. Wienand

et al.

Life Science Alliance, Journal Year: 2024, Volume and Issue: 8(3), P. e202403130 - e202403130

Published: Dec. 12, 2024

Subcellular fractionation allows for the investigation of compartmentalized processes in individual cellular organelles. Nuclear enrichment methods commonly employ use density gradients combined with ultracentrifugation freshly isolated tissues. Although it is broadly used combination proteomics, this approach poses several challenges when comes to scalability and applicability frozen material. To overcome these limitations, we developed FrozONE (Frozen Organ Nucleus Enrichment), a nucleus proteomics workflow By extensively benchmarking our against alternative methods, showed that faster, simpler, more scalable conventional methods. allowed study, profiling, classification nuclear proteomes different tissues complex heterogeneity, ensuring optimal from cell types quantitative resolution low abundant proteins. In addition its performance healthy mouse tissues, proved be very efficient characterization liver proteome alterations pathological condition, diet-induced nonalcoholic steatohepatitis.

Language: Английский

Citations

0
Enhancing human ACE2 expression in mouse models to improve COVID‐19 research DOI Creative Commons

Sun Jiaoyang,

Cheng Shaofei,

Hong Guangliang

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: 15(2), P. 324 - 334

Published: Dec. 29, 2024

Mice are one of the most common biological models for laboratory use. However, wild-type mice not susceptible to COVID-19 infection due low affinity mouse ACE2, entry protein SARS-CoV-2. Although with human ACE2 (hACE2) driven by Ace2 promoter reflect its tissue specificity, these animals exhibit expression, potentially limiting their fidelity in mimicking manifestations and utility viral studies. Here, we created compared hACE2 generated different strategies. Our findings show that distinct β-globin insertion within cassette significantly influences downstream placement enhancing transcription. Moreover, optimizing codons (opt-hACE2) improves translation efficiency multiple tissues. Notably, opt-hACE2 displayed more active immune responses severe phenotypes following SARS-CoV-2 challenge other models. study demonstrates dual regulatory role element transgene transcription suggests might serve as valuable tools research.

Language: Английский

Citations

0