Desulfovibrio vulgaris caused gut inflammation and aggravated DSS-induced colitis in C57BL/6 mice model

Gut Pathogens, Journal Year: 2024, Volume and Issue: 16(1)

Published: July 26, 2024

Abstract Background Sulfate-reducing bacteria (SRB) is a potential pathogen usually detected in patients with gastrointestinal diseases. Hydrogen sulfide (H2S), metabolic byproduct of SRB, was considered the main causative agent that disrupted morphology and function gut epithelial cells. Associated study also showed flagellin from Desulfovibrio vulgaris (DVF), representative bacterium genus, could exacerbate colitis due to interaction DVF LRRC19, leading secretion pro-inflammatory cytokines. However, we still have limited understanding about change microbiota (GM) composition caused by overgrowth SRB its exacerbating effects on colitis. Results In this study, transplanted D. into mice treated or without DSS, set one-week recovery period investigate impact model. The outcomes normal cause inflammation, disrupt barrier reduce level short-chain fatty acids (SCFAs). Moreover, significantly augmented DSS-induced damage inflammatory cytokines, for instance, IL-1β, iNOS, TNF-α. Furthermore, results markedly GM composition, especially decrease relative abundance SCFAs-producing bacteria. Additionally, stimulated growth Akkermansia muciniphila probably via byproduct, H2S, vivo. Conclusions Collectively, indicated transplantation inflammation aggravate induced DSS.

Language: Английский

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Emerging roles of intratumor microbiota in cancer: tumorigenesis and management strategies

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 11, 2024

Language: Английский

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Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excess Desulfovibrio through gut–liver axis

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: June 27, 2024

Diet is a key player in gut–liver axis. However, the effect of different dietary patterns on gut microbiota and liver functions remains unclear. Here, we used rodent standard chow purified diet to mimic two common human patterns: grain plant-based refined-food-based diet, respectively explored their impacts liver. Gut experienced great shift with notable increase Desulfovibrio, bile acid (BA) levels elevated significantly, inflammation was observed mice fed diet. Liver BA also occurred after receiving Desulfovibrio desulfuricans ATCC 29,577 (DSV). Restriction sulfur-containing amino acids (SAAs) prevented injury mainly through higher hepatic antioxidant detoxifying ability reversed due excess Desulfovibrio. Ex vivo fermentation fecal primary BAs demonstrated that DSV enhanced production secondary BAs. Higher concentration both were found germ-free DSV. In conclusion, SAAs may become an effective intervention prevent associated gut.

Language: Английский

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Desulfovibrio vulgaris flagellin exacerbates colorectal cancer through activating LRRC19/TRAF6/TAK1 pathway

Gut Microbes, Journal Year: 2024, Volume and Issue: 17(1)

Published: Dec. 24, 2024

The initiation and progression of colorectal cancer (CRC) are intimately associated with genetic, environmental biological factors. Desulfovibrio vulgaris (DSV), a sulfate-reducing bacterium, has been found excessive growth in CRC patients, suggesting potential role carcinogenesis. However, the precise mechanisms underlying this association remain incompletely understood. We have was abundant high-fat diet-induced Apcmin/+ mice, DSV, member Desulfovibrio, triggered colonocyte proliferation germ-free mice. Furthermore, level DSV progressively rose from healthy individuals to patients. Flagella important accessory structures bacteria, which can help them colonize enhance their invasive ability. that D. flagellin (DVF) drove proliferation, migration, invasion cells fostered xenografts. DVF enriched epithelial-mesenchymal transition (EMT)-associated genes characterized facilitation on EMT. Mechanistically, induced EMT through functional transmembrane receptor called leucine-rich repeat containing 19 (LRRC19). interacted LRRC19 modulate ubiquitination tumor necrosis factor receptor-associated (TRAF)6, rather than TRAF2. This interaction pivotal molecule TAK1, further enhancing its autophosphorylation ultimately contributing Collectively, interacts activate TRAF6/TAK1 signaling pathway, thereby promoting CRC. These data shed new light gut microbiota establish clinical therapeutic target.

Language: Английский

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Gut Microbiome Profiles in Colorectal Cancer Patients in Iraq

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(1), P. 22 - 22

Published: Jan. 16, 2025

Colorectal cancer (CRC) is the third most commonly diagnosed globally, and a significant contributor to both morbidity mortality rates. Emerging research has promptly highlighted potential role of gut microbiome in development progression CRC. This study aims investigate differences microbiota between CRC patients healthy individuals Iraq, using 16S rRNA metagenomic sequencing on Illumina NovaSeq (PE250-Seq). A total 21 stool samples were analyzed: 12 from early-stage nine controls. Bacterial DNA was extracted, followed by amplicon profile microbial communities. The results indicated fecal two groups. Remarkably, exhibited marked reduction Bacteroidota an increase Verrucomicrobiota compared At genus level, Prevotella, Faecalibacterium, Roseburia, Barnesiella, Eubacterium Lachnospiraceae_UCG_004, Lachnospira significantly less abundant individuals, while Actinomyces, Monoglobus, Desulfovibrio, Akkermansia, Bacteroides highly enriched. In addition, diversity analyses further decreased α-diversity distinct β-diversity patterns patients, suggesting shifts composition. These findings underscore microbiome-based diagnostics therapeutic strategies, with alterations serving as biomarkers for diagnosis. Further needs focus elucidating causal relationships these changes management.

Language: Английский

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Intratumoral microbiota, fatty acid metabolism, and tumor microenvironment constitute an unresolved trinity in colon adenocarcinoma

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 20, 2025

The intratumoral microbiota, fatty acid metabolism (FAM), and tumor microenvironment (TME) all provide insights into the management of colon adenocarcinoma (COAD). But biological link among three remains unclear. Here, we analyzed microbiome samples matched host transcriptome from 420 patients with COAD in Cancer Genome Atlas (TCGA). All were divided two subtypes (FAM_high FAM_low) based on Gene set variation analysis (GSVA) score FAM pathway. Furthermore, found significant difference microbiota signatures between subtypes. In-depth suggested that specific microbes tumors may indirectly modify TME, particularly stromal cell populations, by modulating process. More importantly, crosstalk can have a impact prognosis, response to immunotherapy, drug sensitivity patients. Pathological image profiling showed changes TME originating disturbance could be reflected pathological features. In summary, our study provides novel links FAM, COAD, offer guidance for therapeutic opportunities target microbes.

Language: Английский

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De-coding the complex role of microbial metabolites in cancer

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115358 - 115358

Published: March 1, 2025

SummaryThe human microbiome, an intricate ecosystem of trillions microbes residing across various body sites, significantly influences cancer, a leading cause morbidity and mortality worldwide. Recent studies have illuminated the microbiome's pivotal role in cancer development, either through direct cellular interactions or by secreting bioactive compounds such as metabolites. Microbial metabolites contribute to initiation mechanisms DNA damage, epithelial barrier dysfunction, chronic inflammation. Furthermore, microbial exert dual roles on progression response therapy modulating metabolism, gene expression, signaling pathways. Understanding these complex is vital for devising new therapeutic strategies. This review highlights promising targets prevention treatment, emphasizing their impact responses underscoring need further research into metastasis resistance.

Language: Английский

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Fermented guava (Psidium guajava) by Lactiplantibacillus plantarum NCU0011129 attenuates azoxymethane/dextran sodium sulfate-induced colorectal cancer in mice: Restructuring gut microbiota and enhancing intestinal barrier function

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106672 - 106672

Published: April 1, 2025

Language: Английский

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Hand Osteoarthritis: Molecular Mechanisms, Randomized Controlled Trials, and the Future of Targeted Treatment

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4537 - 4537

Published: May 9, 2025

Hand osteoarthritis (OA) is a prevalent and disabling condition, yet its pathogenesis remains less studied than OA in large weight-bearing joints. Emerging genetic, epigenetic, microbiome research suggests that hand might be biologically distinct, involving joint-specific pathways not shared by knee or hip OA. This review integrates genome-wide association studies specific to OA, highlighting key molecular contributors such as inflammatory cytokines. These genetic insights, together with emerging data on epigenetic alterations gut microbial dysbiosis, point broader systemic regulatory influences onset progression. We also assess pharmacologic interventions tested randomized controlled trials have attempted target these pathways. While agents TNF IL-6 inhibitors, hydroxychloroquine, corticosteroids shown limited success, evidence supports the potential of methotrexate synovitis-positive general platelet-rich plasma thumb carpometacarpal (CMC) prolotherapy interphalangeal (IP) findings illustrate persistent gap between mechanistic understanding therapeutic success. Future work must prioritize multifactorial strategies for addressing pain translational frameworks link mechanisms treatment response. In summary, this offers an update identifies opportunities more targeted effective therapy.

Language: Английский

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Desulfovibrio vulgaris exacerbates sepsis by inducing inflammation and oxidative stress in multiple organs

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 30, 2025

Sepsis is a life-threatening condition that often leads to organ dysfunction and systemic inflammation, with gut microbiota dysbiosis playing crucial role in its pathogenesis. The of Desulfovibrio vulgaris (D. vulgaris), potentially pathogenic bacterium, sepsis remains unclear. We first assessed the abundance D. feces septic mice patients using qPCR. Mice were then orally gavaged (2 × 108 CFU/mouse/day) for 7 consecutive days followed by cecal ligation puncture (CLP) surgery. monitored survival, damage, measured inflammation. Peritoneal macrophages isolated analyze phosphorylation key MAPK NF-κB signaling pathways. Finally, oxidative stress levels liver, lungs, kidneys evaluated, measuring markers such as GSH, CAT, SOD. was significantly increased both patients. Supplementation exacerbated mice, resulting lower survival rates, more severe heightened Phosphorylation pathways peritoneal enhanced. Additionally, amplified across multiple organs, indicated ROS decreased antioxidant enzyme activity. Our findings suggest exacerbates progression enhancing activating immune pathways, increasing stress. These processes contribute mortality, highlighting potential sepsis.

Language: Английский

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