Analysis of molecular mechanisms of delafloxacin resistance in Escherichia coli DOI Creative Commons

András Kubicskó,

Katalin Kamotsay,

Dóra Szabó

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Abstract In this study delafloxacin resistance mechanisms in Escherichia coli strains were analyzed. Delafloxacin is a new fluoroquinolone, that approved for clinical application however, against agent scarcely reported. our 37 E. included and antimicrobial susceptibility testing was performed ciprofloxacin, delafloxacin, levofloxacin, moxifloxacin, ceftazidime, cefotaxime, imipenem. Six resistant selected whole-genome sequencing all of them exhibited to other fluoroquinonlones showed an extended-spectrum beta-lactamase phenotype. The six belonged different sequence types (STs) namely, ST131 (2 strains), ST57 ST162 ST15840. Each strain possessed multiple mutations quinolone resistance-determining regions (QRDRs). Notably, three gyrA Ser83Leu, Asp87Asn parC Ser80Ile ST162, However, the two carried five combined Asp87Asn, Ser80Ile, Glu84Val, parE Ile549Leu. Association production CTX-M-15 ST131, CMY-2 ST15840 detected. ST, clonal complex 69 identified. Our results demonstrate, at least QRDRs are required .

Language: Английский

High prevalence of fecal carriage of extended-spectrum beta-lactamase producing Enterobacterales among patients with urinary tract infections in rural Tanzania DOI Creative Commons
Magreth Erick Macha, Weihong Qi, Salomé N. Seiffert

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

The global rise of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-PE) challenges resource-limited countries with insufficient laboratory infrastructure. This study investigates fecal carriage and risk factors for ESBL-PE carbapenemase-producing organisms among patients urinary tract infection (UTI) in rural Tanzania. cross-sectional was conducted at St. Francis Regional Referral Hospital, Ifakara, Tanzania, from October 2021 to August 2023, involving 326 UTI patients. Demographic data resistance were collected via structured questionnaires. Stool samples pre-antibiotic treatment screened carbapenemase locally. Positive underwent further analysis Switzerland using MALDI-ToF, Vitek MS, whole-genome sequencing. Multivariable assessed predictors associated p < 0.05. We enrolled (median age: 35.5 years, range: 25-52) 189 (58.0%) females. Fecal colonization detected 70.9% patients, predominantly E. coli (62.8%) K. pneumoniae (33.0%). Whole-genome sequencing identified diverse phylogroups sequence types, CTX-M-15 being the most common ESBL gene. IncF plasmids primary carriers. Younger age (aOR: 0.98, 95% CI: 0.97-0.99; = 0.0239) inpatient status 1.77, 1.08-2.91; 0.0036) significant carriage. high prevalence Tanzania highlights need improved control research into community transmission dynamics.

Language: Английский

Citations

0
Molecular Characterization of Extended-Spectrum ß-Lactamases-Producing Escherichia coli Isolated from a Greek Food Testing Laboratory DOI Creative Commons

Aikaterini Dikaiou,

Nikolaos Tzimotoudis,

Daniel Sergelidis

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(4), P. 329 - 329

Published: March 21, 2025

Background/Objectives: Over the past decade, extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli have emerged as a significant public health concern due to their potential spread beyond clinical settings and healthy carriers, in various environments, including animal source foods. This study seeks investigate molecular characteristics of ESBL-producing E. strains isolated from food origin, with focus on chromosomal typing, plasmid description associated resistance genes’ genetic environment. Methods: Ninety-seven origin samples were tested for isolates resistant cefotaxime. The resulting then subjected antimicrobial susceptibility testing PCR analysis detect genes. Additional assays, encompassing mating-out procedures, typing utilizing Pulsed-Field Gel Electrophoresis, Multilocus Sequence Typing Analysis, Oxford Nanopore Technology Lite whole sequencing, also conducted. Results: was detected 26 raw specimens, generating percentage 27%. Fourteen current (14%) third generation cephalosporins, producing CTX-M-1, CTX-M-15, CTX-M-55, SHV-12 beta-lactamases. respective genes accompanied by Insertion Sequences ISEcp1 IS26, facilitating transfer. Among plasmids harboring ESBL genes, representatives belonging incI1 incompatibility group prevailed (5/8), followed IncY IncX3. Most proved conjugative. Diversity fingerprints revealed. Conclusions: To best our knowledge, this is first describe ESBLs sourced foods Greece. prevalence confined collection primarily very successful IncI1 plasmids, which not linked specific background. lack association confirms that horizontal transfer plays more role than clonal dissemination ESBL-mediated cephalosporin resistance.

Language: Английский

Citations

0
Unraveling the evolution and global transmission of high level tigecycline resistance gene tet(X) DOI Creative Commons
Chaoqun Yao, Longyang Jin, Qi Wang

et al.

Environment International, Journal Year: 2025, Volume and Issue: 199, P. 109499 - 109499

Published: April 25, 2025

Language: Английский

Citations

0
An Unusual ’Gift’ from Humans: Third-Generation Cephalosporin-Resistant Enterobacterales in migratory birds along the East Asian-Australasian Flyway harboring widespread resistant plasmids DOI Creative Commons

Zile Cheng,

Yiwen Chen, Min Li

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109320 - 109320

Published: Feb. 12, 2025

Migratory birds play a pivotal role in the global dissemination of antimicrobial resistance genes (ARGs), with shorebirds relying on coastal wetlands during their long-distance migrations, environments often contaminated and conducive to ARG transmission. However, systematic investigations into (AMR) remain scarce. During spring autumn 2023, we collected 893 throat cloacal swabs from 480 shorebirds, representing 28 species, at Chongming Dongtan, critical stopover along East Asian-Australasian Flyway. Our analysis identified six strains, including four extra-intestinal pathogenic E. coli (ExPEC) two K. pneumoniae, that exhibited third-generation cephalosporins, three ExPEC strains exhibiting significant virulence Galleria mellonella infection assays. We conjugative plasmids: E042113F_p1, carrying blaCMY-2 gene coli, M50_p2, blaKPC-2 hypervirulent pneumoniae plasmid harboring aerobactin system. Bioinformatic experimental analyses confirmed these plasmids could transfer without any fitness cost, remaining stable for least 30 passages. Surprisingly, genomic tracing revealed among similar E042113F_p1 (blaCMY-2), earliest was Chinese swallow 2015, subsequent detections wild Mongolia (2017), Russia (2018), Australia (2019). Notably, E04-CMY-like/M50-KPC-like predominantly originated human sources, underscoring activity cross-species transmission AMR. This human-mediated elements wildlife posed substantial risk amplifying disseminating AMR through long-range migratory bird movements, highlighting urgent need international collaboration under One Health framework. Integrated surveillance, environmental management, stringent antibiotic stewardship are mitigating risks by spreading across ecosystems.

Language: Английский

Citations

0
What’s up ducks? – antimicrobial resistance of Escherichia coli isolated from duck farm environment in Poland extended with genomic characteristics of cephalosporin-resistant strains DOI
Magdalena Skarżyńska, Magdalena Zając,

Renata Kwit

et al.

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 110492 - 110492

Published: March 1, 2025

Language: Английский

Citations

0
Environmental Risk Factors Contributing to the Spread of Antibiotic Resistance in West Africa DOI Creative Commons
Adenike Adenaya, Adedapo Adedayo Adeniran, Chidera Linus Ugwuoke

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 951 - 951

Published: April 21, 2025

Antibiotic resistance is a well-documented global health challenge that disproportionately impacts low- and middle-income countries. In 2019, the number of deaths attributed to associated with antibiotic in Western Sub-Saharan Africa was approximately 27 115 per 100,000, respectively, higher than other regions worldwide. Extensive research has consistently confirmed persistent presence spread hospitals, among livestock, within food supply chains, across various environmental contexts. This review documents risk factors contributing West Africa. We collected studies from multiple African countries using Web Science PubMed databases. screened them for antibiotic-resistant bacteria genes between 2018 2024. Our findings indicate remains significant concern Africa, pollution waste management identified as major proliferation Additional include poor hygiene, use antibiotics agriculture, aquaculture, animal farming, transmission hospital settings. Unfortunately, lack comprehensive genetic characterization hinders thorough understanding this critical issue region. Since transcends national borders can countries, it essential understand driving its dissemination Such will be instrumental developing recommending effective strategies nationally internationally combat resistance.

Language: Английский

Citations

0
Analysis of molecular mechanisms of delafloxacin resistance in Escherichia coli DOI Creative Commons

András Kubicskó,

Katalin Kamotsay,

Dóra Szabó

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 2, 2024

Abstract In this study delafloxacin resistance mechanisms in Escherichia coli strains were analyzed. Delafloxacin is a new fluoroquinolone, that approved for clinical application however, against agent scarcely reported. our 37 E. included and antimicrobial susceptibility testing was performed ciprofloxacin, delafloxacin, levofloxacin, moxifloxacin, ceftazidime, cefotaxime, imipenem. Six resistant selected whole-genome sequencing all of them exhibited to other fluoroquinonlones showed an extended-spectrum beta-lactamase phenotype. The six belonged different sequence types (STs) namely, ST131 (2 strains), ST57 ST162 ST15840. Each strain possessed multiple mutations quinolone resistance-determining regions (QRDRs). Notably, three gyrA Ser83Leu, Asp87Asn parC Ser80Ile ST162, However, the two carried five combined Asp87Asn, Ser80Ile, Glu84Val, parE Ile549Leu. Association production CTX-M-15 ST131, CMY-2 ST15840 detected. ST, clonal complex 69 identified. Our results demonstrate, at least QRDRs are required .

Language: Английский

Citations

1