Human milk microbiota and oligosaccharides in colostrum and mature milk: comparison and correlation DOI Creative Commons

Hongda Ge,

Wenxiu Zhu,

Jing Zhang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 12, 2024

Background The interaction between the human breast milk microbiota and oligosaccharides (HMOs) plays a crucial role in healthy growth development of infants. We aimed to clarify link HMOs at two stages lactation. Methods 20 colostrum samples (C group, 1–5 days postpartum) mature (S 42 collected from postpartum mothers were analyzed using 16S rRNA gene high-throughput sequencing high-performance liquid chromatography–tandem mass spectrometry. Result total average HMO content was significantly higher C group than S (6.76 ± 1.40 g/L vs. 10.27 2.00 g/L, p < 0.05). Among HMOs, values 2′-fucosyllactose (2′-FL, 1.64 1.54 3.03 1.79 g/L), 3′-sialyllactose (3′-SL, 0.10 0.02 0.21 0.06), 6′-SL (0.22 0.09 0.33 0.11 lacto-N-triaose 2 (LNT2, 0.03 0.01 0.16 0.08 g/L) lower ( 0.05), while that 3′-FL (1.35 1.00 0.41 0.43 diversity structure groups also different Comparative analysis microbial communities revealed Proteobacteria Firmicutes most abundant phyla, both groups, with keystone species Serratia , Streptococcus Staphylococcus ) closely interacting including 3′-SL, 6′-SL, LNT2. In PICRUSt2 functional prediction analysis, exhibited significant reduction expression genes involved several infectious disease pathways. Discussion Our findings support recognition as synbiotic comprising beneficial bacteria prebiotic HMOs.

Language: Английский

What defines a healthy gut microbiome? DOI
Matthias Van Hul, Patrice D. Cani, Camille Petitfils

et al.

Gut, Journal Year: 2024, Volume and Issue: 73(11), P. 1893 - 1908

Published: Sept. 25, 2024

The understanding that changes in microbiome composition can influence chronic human diseases and the efficiency of therapies has driven efforts to develop microbiota-centred such as first next generation probiotics, prebiotics postbiotics, microbiota editing faecal transplantation. Central research is how disease impacts vice versa, yet there a problematic issue with term 'dysbiosis', which broadly links microbial imbalances various illnesses without precision or definition. Another significant discussions defining 'healthy individuals' ascertain what characterises healthy microbiome. This involves questioning who represents healthiest segment our population-whether it those free from illnesses, athletes at peak performance, individuals living healthily through regular exercise good nutrition even elderly adults centenarians have been tested by time achieved remarkable longevity.This review advocates for delineating 'what defines microbiome?' considering broader range factors related health environmental influences on microbiota. A undoubtedly linked gut health. Nevertheless, very difficult pinpoint universally accepted definition 'gut health' due complexities measuring functionality besides composition. We must take into account individual variabilities, diet, lifestyle, host factors. Moreover, challenge distinguishing causation correlation between overall presented.The also highlights resource-heavy nature comprehensive assessments, hinders their practicality broad application. Finally, we call continued nuanced approach better understand intricate evolving concept health, emphasising need more precise inclusive definitions methodologies studying

Language: Английский

Citations

43
Bioactive compounds in human milk DOI
Anthony E. Castro, Maria Sanchez-Holgado, Miguel Sáenz de Pipaón

et al.

Current Opinion in Clinical Nutrition & Metabolic Care, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Purpose of review: Human milk is the optimal food choice for infants. Reviewing latest advances in research about human compounds and their effect on health helps understand benefits breastfeeding improves knowledge key bioactive nutrients that can be used to improve feeding during infancy, with short long-time effects health. Recent findings: In last years, it has been described how such as oligosaccharides, hormones, lipids, cellular components microbes play an important role infants’ health, reducing risk infectious, metabolic autoimmune diseases. The mechanisms transmission from mother infant these are not always well described, but there several lines biological beneficial effects. Summary: These findings may help maternal interventions, modifiable factors able modulate composition. They development formulas enhance nutritional plans. Also, identification isolation describe new ways supplementation.

Language: Английский

Citations

0
Human milk oligosaccharide secretion dynamics during breastfeeding and its antimicrobial role: A systematic review DOI
Mohammed Al‐Beltagi

World Journal of Clinical Pediatrics, Journal Year: 2025, Volume and Issue: 14(2)

Published: March 18, 2025

BACKGROUND Human milk oligosaccharides (HMOs) are bioactive components of breast with diverse health benefits, including shaping the gut microbiota, modulating immune system, and protecting against infections. HMOs exhibit dynamic secretion patterns during lactation, influenced by maternal genetics environmental factors. Their direct indirect antimicrobial properties have garnered significant research interest. However, a comprehensive understanding dynamics their correlation efficacy remains underexplored. AIM To synthesize current evidence on lactation evaluate roles bacterial, viral, protozoal pathogens. METHODS A systematic search PubMed, Scopus, Web Science, Cochrane Library focused studies investigating natural synthetic HMOs, dynamics, properties. Studies involving human, animal, in vitro models were included. Data HMO composition, temporal patterns, mechanisms action extracted. Quality assessment was performed using validated tools appropriate for study design. RESULTS total 44 included, encompassing research. exhibited 2′-fucosyllactose (2′-FL) lacto-N-tetraose peaking early declining over time, while 3-fucosyllactose (3-FL) increased later stages. demonstrated through pathogen adhesion inhibition, biofilm disruption, enzymatic activity impairment. Synthetic bioengineered 2′-FL 3-FL, structurally functionally comparable to effectively inhibiting pathogens such as Pseudomonas aeruginosa , Escherichia coli Campylobacter jejuni . Additionally, synergistic effects antibiotics, enhancing resistant CONCLUSION vital defense, supporting infant targeting various Both hold potential therapeutic applications, particularly nutrition adjuncts antibiotics. Further research, clinical trials, is essential address gaps knowledge, validate findings, explore broader applicability improving neonatal health.

Language: Английский

Citations

0
Comprehensive review on human Milk oligosaccharides: Biosynthesis, structure, intestinal health benefits, immune regulation, neuromodulation mechanisms, and applications DOI

Jieting Yu,

Le Li, Supaluck Kraithong

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 209, P. 116328 - 116328

Published: April 6, 2025

Language: Английский

Citations

0
Gut-mammary pathway: Breast milk microbiota as a mediator of maternal gut microbiota transfer to the infant gut DOI Creative Commons

Lulu Meng,

Hang Xie, Zhe Li

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 124, P. 106620 - 106620

Published: Dec. 13, 2024

Language: Английский

Citations

1
Human Milk Microbiota and Oligosaccharides: Origin, Structure, Impact Factors, and Benefits for Infant Health DOI

Xiaojing Wei,

Leilei Yu,

Jianxin Zhao

et al.

Food Reviews International, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Aug. 5, 2024

Early life is an essential period for the establishment of gut microbiota and considered a critical window intervention. Breast milk serves as natural food babies contains variety bioactive ingredients that promote healthy growth. The active in breast are closely related to infant development, such human (HMM), primary source microbiota. Human oligosaccharides (HMOs) can reach colon intact serve fermentable substrates specific microbiota, thereby helping shape absence these formula strongly associated with health problems abnormal infants. This review provides overview changes host at various stages summarizing recent research on early-life ingested food-breast milk, including HMM HMOs, their origin, structure, impact factors, contribution benefits. It also reviews major strategies utilizing HMOs probiotics next-generation potential probiotics, providing reference later development nutritional products.

Language: Английский

Citations

0
Human Milk Metals and Metalloids Shape Infant Microbiota DOI Creative Commons
Eduard Flores Ventura, Manuel Bernabeu, Belén Callejón‐Leblic

et al.

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Human milk metals and metalloids are variable significantly correlate with infant gut microbiota abundance alpha diversity indices.

Language: Английский

Citations

0
Human milk microbiota and oligosaccharides in colostrum and mature milk: comparison and correlation DOI Creative Commons

Hongda Ge,

Wenxiu Zhu,

Jing Zhang

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 12, 2024

Background The interaction between the human breast milk microbiota and oligosaccharides (HMOs) plays a crucial role in healthy growth development of infants. We aimed to clarify link HMOs at two stages lactation. Methods 20 colostrum samples (C group, 1–5 days postpartum) mature (S 42 collected from postpartum mothers were analyzed using 16S rRNA gene high-throughput sequencing high-performance liquid chromatography–tandem mass spectrometry. Result total average HMO content was significantly higher C group than S (6.76 ± 1.40 g/L vs. 10.27 2.00 g/L, p < 0.05). Among HMOs, values 2′-fucosyllactose (2′-FL, 1.64 1.54 3.03 1.79 g/L), 3′-sialyllactose (3′-SL, 0.10 0.02 0.21 0.06), 6′-SL (0.22 0.09 0.33 0.11 lacto-N-triaose 2 (LNT2, 0.03 0.01 0.16 0.08 g/L) lower ( 0.05), while that 3′-FL (1.35 1.00 0.41 0.43 diversity structure groups also different Comparative analysis microbial communities revealed Proteobacteria Firmicutes most abundant phyla, both groups, with keystone species Serratia , Streptococcus Staphylococcus ) closely interacting including 3′-SL, 6′-SL, LNT2. In PICRUSt2 functional prediction analysis, exhibited significant reduction expression genes involved several infectious disease pathways. Discussion Our findings support recognition as synbiotic comprising beneficial bacteria prebiotic HMOs.

Language: Английский

Citations

0