The characteristics of capillary remodeling in cerebellar neurodegenerative diseases revealed through layered imaging and stereoscopic analysis DOI Creative Commons
Yayun Wang, Hui Liu,

Ziwei Ni

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Neurodegenerative diseases refer to a group of clinical conditions characterized by progressive neuronal loss, resulting in impaired brain structural integrity and functional abnormalities. These can lead widespread cerebrovascular remodeling; however, the spatial remodeling features capillaries with diameters ≤ 10 μm remain poorly understood, particularly regard changes relationship between neurons capillaries. In this study, we first developed double-fluorescent transgenic mouse model cerebellar neurodegenerative disease (CBND), PCKOTomatoVesselGreen mouse, which Purkinje cells (PCs) cerebellum express red fluorescent protein, while system expresses green protein (with no differentiation arteries veins). Subsequently, employed whole-brain clearing combined Amira/Imaris conduct three-dimensional layered imaging computational analysis network both adult control mice. A total 181744 PCs vessels length 17.7363 meters, 266175 segments, volume 0.5314 mm³ were analyzed. Compared Control mice, mice exhibited 93% reduction count PCs, 97% 69% volume, 58% decrease vessel length, 52% vascular volume. Depth revealed 55%, 58%, capillary chord curved tortuosity, respectively, statistical differences node or φ θ directional values. Further fraction (VF) 59% increase capillary-cerebellum VF, PC-vessel PC-capillary PC-noncapillary VF decreased 95%, 96%, respectively. Additionally, shortest distance vessel-cerebellum noncapillary-cerebellum showed differences. Our results indicated that significantly lost, their topology remained stable, decreasing from 16 7 μm. This process is central pathogenesis CBND. Furthermore, may serve as biological markers for early diagnosis findings provide foundation development targeted therapies Short abstract Cerebrovascular caused be used diagnosis, but its characteristics are unclear. research constructed disease, then adopted whole transparency system. m, number segments mm3 analyzed stratified analysis. 3.15 TB data lost although stable. blood μm, identifying feature neurovascular disease. The cell-vessel diseases.

Language: Английский

Dual-Modal Super-Resolution Ultrasound and NIR-II Fluorescence Imaging of Ischemic Stroke with ICG-doped Porous PLGA Microspheres DOI Creative Commons
Ziyue Li, Qiang Yu,

Dongli Chen

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101513 - 101513

Published: Jan. 22, 2025

Language: Английский

Citations

0
The characteristics of capillary remodeling in cerebellar neurodegenerative diseases revealed through layered imaging and stereoscopic analysis DOI Creative Commons
Yayun Wang, Hui Liu,

Ziwei Ni

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Neurodegenerative diseases refer to a group of clinical conditions characterized by progressive neuronal loss, resulting in impaired brain structural integrity and functional abnormalities. These can lead widespread cerebrovascular remodeling; however, the spatial remodeling features capillaries with diameters ≤ 10 μm remain poorly understood, particularly regard changes relationship between neurons capillaries. In this study, we first developed double-fluorescent transgenic mouse model cerebellar neurodegenerative disease (CBND), PCKOTomatoVesselGreen mouse, which Purkinje cells (PCs) cerebellum express red fluorescent protein, while system expresses green protein (with no differentiation arteries veins). Subsequently, employed whole-brain clearing combined Amira/Imaris conduct three-dimensional layered imaging computational analysis network both adult control mice. A total 181744 PCs vessels length 17.7363 meters, 266175 segments, volume 0.5314 mm³ were analyzed. Compared Control mice, mice exhibited 93% reduction count PCs, 97% 69% volume, 58% decrease vessel length, 52% vascular volume. Depth revealed 55%, 58%, capillary chord curved tortuosity, respectively, statistical differences node or φ θ directional values. Further fraction (VF) 59% increase capillary-cerebellum VF, PC-vessel PC-capillary PC-noncapillary VF decreased 95%, 96%, respectively. Additionally, shortest distance vessel-cerebellum noncapillary-cerebellum showed differences. Our results indicated that significantly lost, their topology remained stable, decreasing from 16 7 μm. This process is central pathogenesis CBND. Furthermore, may serve as biological markers for early diagnosis findings provide foundation development targeted therapies Short abstract Cerebrovascular caused be used diagnosis, but its characteristics are unclear. research constructed disease, then adopted whole transparency system. m, number segments mm3 analyzed stratified analysis. 3.15 TB data lost although stable. blood μm, identifying feature neurovascular disease. The cell-vessel diseases.

Language: Английский

Citations

0