Enhanced and Prolonged Immunogenicity in Mice of Thermally Stabilized Fatty Acid-Conjugated Vaccine Antigen

Vaccines, Journal Year: 2025, Volume and Issue: 13(2), P. 168 - 168

Published: Feb. 10, 2025

Background/Objectives: Influenza vaccines require good thermal stability without the need for refrigerator storage. Although fatty acid-conjugated hemagglutinin (Heg) vaccine antigen provides in both solid and liquid states, its therapeutic effectiveness must be validated vivo. This study aimed to investigate immunogenicity of thermally stabilized Heg-oleic acid conjugate (HOC) compare it with native Heg as a reference. Method: To evaluate HOC immunogenicity, an enzyme-linked immunosorbent assay was used measure inhibition (HI) titers, serum IgG antibody titers (IgG1, IgG2a), cytokine secretion levels (IFN-γ, IL-4) BALB/c mice after intramuscular (IM) injection. Results: Thermally induced higher more sustained IgG1 IgG2a responses than antigen. is typically associated Th2 response, whereas Th1 response. appeared enhance responses, inducing balanced immune Moreover, antigens stimulate broader suggesting stronger longer-lasting memory. The IFN-γ (2.8-fold) IL-4 (6-fold) were significantly increased HOC-immunized group compared group. IFN-γ, that activates demonstrated enhanced ability induce IL-4, promotes indicated also strongly crucial maintaining their structural integrity, enabling continuous exposure stable denaturation. allows cells recognize efficiently form long-term Conclusions: processing efficiency antigen-presenting (APCs) stimulated responses. could provide improved storage but immunogenic efficacy antigen, supporting potential further applications.

Language: Английский

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An mRNA vaccine candidate encoding cholera toxin subunit B and conserved antigens of influenza viruses confers cross-protection against influenza a viruses in adult and aged mice

Human Vaccines & Immunotherapeutics, Journal Year: 2025, Volume and Issue: 21(1)

Published: Feb. 16, 2025

Currently, vaccination with influenza vaccines is still an effective strategy to prevent infection by seasonal virus. However, frequently fail induce immune protection against rapidly changing viruses and emerging zoonotic viruses. In addition, may not confer potent in elderly immunocompromised individuals. There urgent need develop broad-spectrum address this problem. Herein, we designed mRNA-based vaccine candidate encoding cholera toxin subunit B conserved antigens of both adult aged mice, universal mRNA stimulated robust T-cell humoral responses conferred mice.

Language: Английский

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A humanised ACE2, TMPRSS2, and FCGRT mouse model reveals the protective efficacy of anti-receptor binding domain antibodies elicited by SARS-CoV-2 hybrid immunity

EBioMedicine, Journal Year: 2025, Volume and Issue: 113, P. 105619 - 105619

Published: Feb. 27, 2025

Language: Английский

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Design, optimization, and evaluation of lyophilized lipid nanoparticles for mRNA-based pulmonary mucosal vaccination

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101813 - 101813

Published: May 1, 2025

Language: Английский

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Breaking down IgA: Tomasiella immunophila enlightens microbiome–immune interactions

Trends in Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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