The Mechanical Role of YAP/TAZ in the Development of Diabetic Cardiomyopathy DOI Creative Commons
Junxian Shen, Ling Zhang,

Huan‐Huan Liu

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 297 - 297

Published: April 23, 2025

Diabetic cardiomyopathy (DCM) begins with a subclinical stage featuring cardiac hypertrophy, fibrosis, and disrupted signaling. These changes, especially fibrosis stiffness, often lead to clinical heart failure. The mechanism involves metabolic dysregulation, oxidative stress, inflammation, leading damage dysfunction. During the progression of disease, myocardium senses surrounding mechanical cues, including extracellular matrix properties, tensile tension, shear pressure load, which significantly influence pathological remodeling through mechanotransduction. At molecular level, mechanisms by cues are sensed transduced mediate myocardial in DCM remain unclear. mechanosensitive transcription factors YAP TAZ fill this gap. This article reviews latest findings how perceive wide range from stress stiffness. We focus on these relayed cytoskeleton nucleus, where they trigger downstream gene expression. Here, we review recent progress crucial role mechanotransduction changes observed DCM, mitochondrial dysfunction, cell death.

Language: Английский

Heterogeneous focal adhesion cytoskeleton nanoarchitectures from microengineered interfacial curvature to oversee nuclear remodeling and mechanotransduction of mesenchymal stem cells DOI Creative Commons
Huayu Fan,

Hui Feng Zhao,

Yan Hou

et al.

Cellular & Molecular Biology Letters, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 24, 2025

Abstract Background Interfacial heterogeneity is widely explored to reveal molecular mechanisms of force-mediated pathways due biased tension. However, the influence cell density,, curvature, and interfacial on underlying mechanotransduction obscure. Methods Polydimethylsiloxane (PDMS)-based stencils were micropatterned prepare micropores for culture. The colonies human mesenchymal stem cells (hMSCs) formed by controlling seeding density investigate influences density, curvature mechanotransduction. Immunofluorescent staining integrin, vinculin, talin-1 was conducted evaluate adhesion-related expression levels. Then, immunofluorescent actin, actinin, myosin performed detect cytoskeleton distribution, especially at periphery. Nuclear force-sensing explained yes-associated protein (YAP) laminA/C analysis. Results colony hMSCs demonstrated coincident characters with engineered microstencils. obviously developed heterogeneous morphogenesis. Heterogeneous focal adhesion guided development together regulate cellular contractility movement talin-1. Cytoskeletal showed that fibers reorganized periphery YAP nuclear translocation remodeling enhanced regulation (FA) arrangement. Conclusions clustering similar results prepared microstencils, also well adjusted establish balance colony. mechanism spreading, elongation investigated disclose compliance FA along microarrays increased may provide helpful information understanding cells.

Language: Английский

Citations

2
Functional nanoplatform for modulating cellular forces to enhance antitumor immunity via mechanotransduction DOI
Wanheng Zhang, Shuqin Liu,

Yan Hou

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 850 - 865

Published: Jan. 29, 2025

Language: Английский

Citations

1
Advanced Microarrays as Heterogeneous Force‐Remodeling Coordinator to Orchestrate Nuclear Configuration and Force‐Sensing Mechanotransduction in Stem Cells DOI Creative Commons
Nana Wang,

Yan Hou,

Xin Lin

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Integrin and focal adhesion can regulate cytoskeleton distribution to govern actin-related force remodeling play an important role in nuclear configuration force-sensing mechanotransduction of stem cells. However, further exploration the interaction between actinin complex myosin, kinetics, molecular mechanism structures nucleate within engineered cells is vague. An extensive comprehension cell morphogenesis, remodeling, essential reveal basic physical principles polymerization force-related signaling delivery. Advanced microarrays are designed determine heterogeneous morphology behaviors The heterogeneity from transferred into nuclei by evaluation Lamins, YAP, BrdU expression. Tuning activation proteins nucleators adjust mechanics may be underlying change response its physiological microarrayed

Language: Английский

Citations

1
Injectable hydrogel with miR-222-engineered extracellular vesicles ameliorates myocardial ischemic reperfusion injury via mechanotransduction DOI Creative Commons
Yong-Tao Wang,

Danni Meng,

Xiaohui Shi

et al.

Cell Reports Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 101987 - 101987

Published: March 1, 2025

Cardiac ischemic reperfusion injury (IRI) significantly exacerbates cardiac dysfunction and heart failure, causing high mortality. Despite the severity of IRI, effective therapeutic strategies remain elusive. Acellular patches have shown considerable efficacy in delivering therapeutics directly to tissues. Herein, we develop injectable GelMA (GEL) hydrogels with controlled mechanical properties. Targeting miR-222-engineered extracellular vesicles (TeEVs), tailored cardiac-ischemia-targeting peptides (CTPs), are developed as TeEV therapeutics. These TeEVs encapsulated within create TeEV-loaded patches, enabling minimal invasiveness attenuate IRI. The facilitate precise targeting for efficient rescue damaged cells. Persistent delivery into infarcted region alleviates acute IRI mitigated remodeling post This is linked focal adhesion activation, cytoskeleton force enhancement, nuclear force-sensing preservation. findings may pave way approaches therapy using bioengineered patches.

Language: Английский

Citations

0
Multidimensional excavation of the current status and trends of mechanobiology in cardiovascular homeostasis and remodeling within 20 years DOI Creative Commons

Wei Liao,

Yu‐Xi Huang,

Xiangxiu Wang

et al.

Mechanobiology in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 100127 - 100127

Published: March 1, 2025

Language: Английский

Citations

0
The Mechanical Role of YAP/TAZ in the Development of Diabetic Cardiomyopathy DOI Creative Commons
Junxian Shen, Ling Zhang,

Huan‐Huan Liu

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 297 - 297

Published: April 23, 2025

Diabetic cardiomyopathy (DCM) begins with a subclinical stage featuring cardiac hypertrophy, fibrosis, and disrupted signaling. These changes, especially fibrosis stiffness, often lead to clinical heart failure. The mechanism involves metabolic dysregulation, oxidative stress, inflammation, leading damage dysfunction. During the progression of disease, myocardium senses surrounding mechanical cues, including extracellular matrix properties, tensile tension, shear pressure load, which significantly influence pathological remodeling through mechanotransduction. At molecular level, mechanisms by cues are sensed transduced mediate myocardial in DCM remain unclear. mechanosensitive transcription factors YAP TAZ fill this gap. This article reviews latest findings how perceive wide range from stress stiffness. We focus on these relayed cytoskeleton nucleus, where they trigger downstream gene expression. Here, we review recent progress crucial role mechanotransduction changes observed DCM, mitochondrial dysfunction, cell death.

Language: Английский

Citations

0