Cited by Molecular identification of TRAF4 gene in aquatic keystone species Daphnia magna and its response to toxic cyanobacteria challenge

Identification and verification of autophagy-related gene signatures and their association with immune infiltration and drug responsiveness in epilepsy DOI

H. He,

X Zhang,

Fenfang Chen

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 22, 2025

Background Epilepsy, a common neurological disorder, is characterized by susceptibility to recurrent seizures. Increasing evidence suggests that autophagy plays crucial role in the initiation and progression of epilepsy. However, precise mechanisms which deficiencies involved epileptogenesis are still not fully understood. Methods Two datasets epilepsy (GSE143272 GSE256068) were downloaded from Gene Expression Omnibus (GEO) database. Differential expression genes (DEGs) analysis weighted gene co-expression network (WGCNA) employed screen for related differential (ARDEGs) GSE143272 Subsequently, protein–protein interaction, transcription factors miRNAs networks constructed. Additionally, functional enrichment Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) applied. The hub ARDEGs identified through CytoHubba, followed LASSO analysis. Immune Cell Abundance Identifier (ImmuCellAI) was used estimate peripheral immune cells abundance Furthermore, level detected patients treated with different monotherapies explore responsiveness antiepileptic drug therapy. Finally, further validated hippocampus GSE256068 enhance reliability results. Results Twenty screened out integrating DEGs WGCNA KEGG showed only autophagy, but also apoptosis, NOD-like receptor signaling pathway, neurotrophin etc. Four (PIK3R1, TRIM21, TRIM22, ITPR3) CytoHubba plug infiltration there significantly increased macrophages decreased CD4 CD8 T cells, including Tr1, nTreg, Tfh, naïve, cytotoxic effector memory group. correlated cells. In validation anti-epileptic analysis, PIK3R1 ITPR3 had significant differences found be carbamazepine resistance. Conclusion This study elucidated autophagy-related signatures clarified their association responsiveness, providing novel target future therapeutic interventions disease markers

Language: Английский

Citations

Effects of Alexandrium pacificum Exposure on Exopalaemon carinicauda: Hepatopancreas Histology, Antioxidant Enzyme Activity, and Transcriptome Analysis DOI

Wanyu Han,

Weitao Cheng,

Menghao Fan

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1605 - 1605

Published: Feb. 13, 2025

Alexandrium pacificum, a dinoflagellate known for causing harmful algal blooms (HABs), has garnered significant attention due to its potential toxicity marine ecosystems, fisheries, and human health. However, the effects of this toxin-producing alga on shrimp are not yet comprehensively understood. This study aimed assess hepatopancreas damage induced by A. pacificum in economically important species E. carinicauda elucidate underlying molecular mechanisms through histology, antioxidant enzyme activity, transcriptome analysis. The were assigned either control group or an exposed group, with latter involving exposure at concentration 1.0 × 104 cells/mL 7 days. A histological analysis subsequently revealed pathological changes tissue including lumen expansion separation basement membrane from epithelial cells, while activity assays demonstrated that weakened defense system, as evidenced reduced activities catalase, superoxide dismutase, glutathione, along increased malondialdehyde levels. Transcriptome further identified 663 significantly upregulated genes 1735 downregulated ones these differentially expressed being primarily associated pathways such protein processing endoplasmic reticulum, mitophagy, glycolysis/gluconeogenesis, sphingolipid metabolism, glycerophospholipid metabolism. provides novel insights into toxicological aquatic organisms enhances current understanding ecotoxicological risks posed HABs.

Language: Английский

Citations

Screening, identification and functional validation of Microcystin-LR direct binding target proteins based on thermal proteomics profiling DOI

Minghao Yan,

C.P. Wang,

Huifang Wu

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 178047 - 178047

Published: Dec. 14, 2024

Language: Английский

Citations

Molecular identification of TRAF4 gene in aquatic keystone species Daphnia magna and its response to toxic cyanobacteria challenge DOI

Yuchen Fan, Jiaxing Huang, Shan Huang

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 369, P. 143856 - 143856

Published: Dec. 1, 2024

Language: Английский

Citations