Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from Ginkgo biloba L. Seeds DOI Creative Commons

Ruisheng Hu,

Zhuo Liu,

Yi Zhou

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 409 - 409

Published: Jan. 19, 2025

The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed utilized for countercurrent from Ginkgo biloba L. seeds. Five were effectively isolated using (2:2:0.8:3, v/v) two-step method. initial process, three successfully purified methanol extract: compound 1, 4, 5. Compounds 2 3, co-eluted column, further subjected to inner-recycling chromatographic procedures. At last, five identified, including 4′-O-methylpyridoxine (1); two indole alkaloid N-glucosides, ginkgoside B (2) A (3); 2-(4-hydroxybenzyl) malic acid (4); coniferyl alcohol (5). results demonstrated that serves feasible efficient components.

Language: Английский

Recent advancement in mass based plant metabolomics: techniques, tools, and analytical approaches DOI

Nitish Kumar,

Jare Shrikrushna Bharat,

Amit Kumar

et al.

Phytochemistry Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Language: Английский

Citations

3
Screening of Solvent Systems for Countercurrent Chromatography Separation of Polar Constituents from Ginkgo biloba L. Seeds DOI Creative Commons

Ruisheng Hu,

Zhuo Liu,

Yi Zhou

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 409 - 409

Published: Jan. 19, 2025

The separation of large polar constituents presents a substantial challenge in natural product research when employing column chromatography techniques, as the process is both complex and time-consuming. In this study, an acetonitrile/tetrahydrofuran/di-(2-ethylhexyl) phosphoric acid/aqueous saturated sodium chloride solvent system was developed utilized for countercurrent from Ginkgo biloba L. seeds. Five were effectively isolated using (2:2:0.8:3, v/v) two-step method. initial process, three successfully purified methanol extract: compound 1, 4, 5. Compounds 2 3, co-eluted column, further subjected to inner-recycling chromatographic procedures. At last, five identified, including 4′-O-methylpyridoxine (1); two indole alkaloid N-glucosides, ginkgoside B (2) A (3); 2-(4-hydroxybenzyl) malic acid (4); coniferyl alcohol (5). results demonstrated that serves feasible efficient components.

Language: Английский

Citations

0