Coix Seed Oil Alleviates DSS-Induced Ulcerative Colitis via Intestinal Barrier Repair and Ferroptosis Regulation

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2557 - 2581

Published: Feb. 1, 2025

Ulcerative colitis is a chronic intestinal disease linked to barrier damage, ferroptosis and dysbiosis. Coix lacryma-jobi natural food with food-medicine homology, whose seed-derived oil (Coix seed oil, CSO) has been shown anti-inflammatory activity in vitro. Here, the effects mechanisms of CSO on ulcerative (UC) vivo are systematically investigated. Firstly, UC mice was replicated by 3% DSS, assessed efficacy observing fecal occult blood, colon length, DAI score pathological histomorphological changes tissues. The barrier-protective were observed AB staining qRT-PCR. Secondly, biological targets obtained from TCMSP database Swiss Target Prediction database, downloaded FerrDb platform, UC-related GEO intersection above three taken obtain "CSO-UC-Ferroptosis" targets, which analysed GO KEGG enrichment, GSEA analysis, immune cell infiltration validation. Finally, core genes molecular docking potential active components CSO. In order further verify effect ferroptosis, GPX4 agonist RSL-3 used stimulate vivo, levels Iron, MDA SOD measured, immunohistochemistry detect tight junction proteins protein mice. Besides, evaluated intercellular junctions tissues each group under electron microscope. addition, 16sRNA sequencing performed contents observe flora improved physiological parameters, reduced inflammation response regulated restored gut microbiota balance Bioinformatics results showed that G6PD, ABCC1 at CSO, also demonstrated similar expression DSS-induced models vivo. Our findings demonstrate for first time ameliorated regulating mice, suggesting as promising candidate treatment warranting investigation.

Language: Английский

Natural Phenolic Compounds against Trichothecenes: From Protective Mechanisms to Future Perspectives

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Trichothecenes (TCNs), Fusarium-derived mycotoxins exemplified by deoxynivalenol and T-2 toxin, threaten global health through multisystem toxicity widespread contamination. Natural phenolic compounds (NPCs), leveraging their intrinsic safety natural abundance, demonstrate multimechanistic efficacy in counteracting TCN toxicity. This article reviews both domestic international research on the protective mechanisms of NPCs against TCN-induced exert effects multitiered mechanisms: (1) molecular regulation via Nrf2-centric antioxidant activation MAPK/NF-κB inflammatory axis suppression, coupled with coordinated inhibition programmed cell death pathways (apoptosis/ferroptosis/pyroptosis) autophagy modulation, where GPX4 emerges as a critical ferroptosis regulator; (2) restoring microbiome balance, enhancing intestinal barrier function, optimizing nutrient transport. Gut microflora may also serve an additional target for mitigating TCNs. further inhibit Fusarium proliferation mycotoxin biosynthesis. While there is demonstrated potential food sustainable feed development, challenges persist bioavailability optimization, pharmacokinetic profiling, microbiota-metabolite crosstalk. analysis advances NPC-based strategies detoxification agriculture.

Language: Английский

Quercetin Simultaneously Treats Skeletal Fluorosis and Kashin-Beck Disease by Modulating HIF-1 and Ferroptosis Signaling Pathways

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский