Microbial community structure and metabolic characteristics in the five different hadal trenches DOI Creative Commons
Hongmei Jing, Hao Liu, Yao Xiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

Abstract Microbial community growth efficiency, the ratio of production to substrate assimilated, could provide insights into carbon flow among microbes and regulation marine biogeochemical cycles. However, by far microbial metabolic characters were largely undetermined in deep hadal trench. Here, structural metabolism characteristics communities five different trenches investigated using Illumina high-throughput sequencing quantitative PCR, as well incubation with 3H-leucine incorporation method electron transport system. The structure diversity located hemispheres significantly different, higher gene abundance appear northern southern hemispheres, respectively. Depth, TOC TP identified key factors. Cooperative relationship existed groups demonstrated co-occurrence network Pearson correlation analysis. respiration rates hemisphere than those under atmospheric pressure. prokaryotic efficiencies (PGE) pressure high hydrostatic pressure, this negative effect possibly because was more inclined maintain This study represented first comprehensive investigation sediments trenches, providing a preliminary insight processes efficiency microbial-driven cycles biosphere.

Language: Английский

Metagenome sequencing and 107 microbial genomes from seamount sediments along the Yap and Mariana trenches DOI Creative Commons
Yue Zhang, Hongmei Jing

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Aug. 15, 2024

Microbes in the sediments across a series of seamounts along island arc Yap and Mariana trenches were investigated by metagenome. In this study, we reconstructed 107 metagenome-assembled genomes (MAGs), including 100 bacteria 7 archaea. All MAGs exhibited >75% completeness <10% contamination, with 26 being classified as 'nearly complete' (completeness >90%), while 50 falling within 80–90% range 31 between 75–80% complete. Phylogenomic analysis revealed that 86% (n = 92) these represented new taxa at different taxonomical levels. The species composition was most consistent previous reports, abundant phyla Proteobacteria 39), Methylomirabilota 27), Nitrospirota 7). These draft provided novel data on diversity function seamount microbial community, which will provide reference for extensive comparative genomic studies crucial phylogenetic groups worldwide.

Language: Английский

Citations

1
Geographical distribution and driving force of microbial communities in the sediments of Diamantina and Kermadec trenches DOI Creative Commons
Yue Zhang, Hongmei Jing, Hao Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 18, 2024

The distinctive geological characteristics of hadal trenches are recognized to affect the construction and ecological role microbial communities; however, information on their population dynamics assembly processes remains limited. In this study, bacteria micro-eukaryotes in sediments Diamantina Kermadec were explored utilizing high-throughput sequencing. Compared Trench, significantly lower levels bacterial micro-eukaryotic biodiversity ( p &lt; 0.01), gene copy number 0.05), heterotrophic/parasitic proportions 0.05) detected which also exhibited a low community complexity based network analysis. Within each trench, no obvious shifts observed along trench axis. Microbial communities both showed clear distance–decay distributions, mainly driven by stochastic processes. This study provided fresh perspectives mechanism deep-sea trenches. Studies diversified trophic states microbes would contribute an improved understanding functions diversification extreme biosphere.

Language: Английский

Citations

1
Microbial community structure and metabolic characteristics in the five different hadal trenches DOI Creative Commons
Hongmei Jing, Hao Liu, Yao Xiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

Abstract Microbial community growth efficiency, the ratio of production to substrate assimilated, could provide insights into carbon flow among microbes and regulation marine biogeochemical cycles. However, by far microbial metabolic characters were largely undetermined in deep hadal trench. Here, structural metabolism characteristics communities five different trenches investigated using Illumina high-throughput sequencing quantitative PCR, as well incubation with 3H-leucine incorporation method electron transport system. The structure diversity located hemispheres significantly different, higher gene abundance appear northern southern hemispheres, respectively. Depth, TOC TP identified key factors. Cooperative relationship existed groups demonstrated co-occurrence network Pearson correlation analysis. respiration rates hemisphere than those under atmospheric pressure. prokaryotic efficiencies (PGE) pressure high hydrostatic pressure, this negative effect possibly because was more inclined maintain This study represented first comprehensive investigation sediments trenches, providing a preliminary insight processes efficiency microbial-driven cycles biosphere.

Language: Английский

Citations

0