Understanding the Molecular Mechanism of PGPR Strain Priestia megaterium from Tea Rhizosphere for Stress Alleviation and Crop Growth Enhancement
Plant Stress,
Journal Year:
2024,
Volume and Issue:
12, P. 100494 - 100494
Published: May 19, 2024
The
study
aimed
to
assess
the
plant
growth-promoting
(PGP)
capabilities
and
abiotic
stress
tolerance
of
strain
IHB
B
7164,
with
a
focus
on
identifying
associated
genes
through
genomic
analysis.
Isolated
from
tea
rhizosphere,
underwent
screening
for
PGP
traits
against
various
factors.
Taxonogenomic
analysis
complete
genome
identified
bacterium
as
Priestia
megaterium,
exhibiting
high
similarity
(97.04%
ANI,
74.3%
in
silico
DDH)
P.
megaterium
ATCC
14581.
Functional
annotations
highlighted
gene
clusters
tolerance,
substantiating
strain's
potential
promoting
growth
under
conditions.
Under
stressed
culture
conditions
such
acidity/alkalinity,
temperature
fluctuations,
desiccation,
salinity,
exposure
calcium,
aluminum,
iron
salts,
demonstrated
robust
activities,
affirming
its
status
broad-spectrum
rhizobacterium.
Screening
acidic
revealed
phosphate
solubilization,
ACC-deaminase
activity
production
IAA-like
auxins.
Notably,
gluconic
oxalic
acids
were
consistently
detected
during
solubilization
tri-calcium
(TCP),
aluminum
(Al-P),
(Fe-P).
Inoculation
led
significant
enhancements
nursery
plants,
including
9%
increase
height,
10%
leaf
number,
substantial
improvements
fresh
dry
weights.
Similarly,
pea
wheat
yields
recorded
notable
increases
16.9%
18.3%,
respectively,
field
These
findings
underscore
efficacy
7164
an
stress-tolerant
rhizobacteria
(PGPR)
capable
enhancing
productivity
across
diverse
agricultural
settings.
Language: Английский
Effect of intra- and inter-specific plant interactions on the rhizosphere microbiome of a single target plant at different densities
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(1), P. e0316676 - e0316676
Published: Jan. 27, 2025
Root
and
rhizosphere
studies
often
focus
on
analyzing
single-plant
microbiomes,
with
the
literature
containing
minimum
empirical
information
about
shared
microbiome
of
multiple
plants.
Here,
individual
plants
was
analyzed
in
a
microcosm
study
different
combinations
densities
(1–3
plants,
24
48
plants)
cover
crops:
Medicago
sativa
,
Brassica
sp.,
Fescue
sp.
Rhizobacterial
beta
diversity
reduced
by
increasing
plant
density
for
all
mixtures.
Interestingly,
had
significant
influence
over
while
found
to
be
less
important
factor
since
it
did
not
have
change.
Regardless
neighbor
identity
or
density,
low
number
rhizobacteria
were
strongly
associated
each
target
species.
Nonetheless,
few
bacterial
taxa
shown
conditional
associations
such
as
being
enriched
within
only
high
densities,
which
may
alleviate
competition
between
these
Also,
we
evidence
sharing
nitrogen
fixers
from
alfalfa
fescue.
Although
networks
overlapping
modules,
modules
showing
largest
percentage
network
changed
depending
neighbor.
In
summary,
this
that
most
part
maintained
their
despite
escalating
plant-plant
competition.
Language: Английский
Genome Mining of Pseudarthrobacter sp. So.54, a Rhizospheric Bacteria from Colobanthus quitensis Antarctic Plant
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(4), P. 534 - 534
Published: April 5, 2025
Antarctic
microorganisms
have
genomic
characteristics
and
biological
functions
to
ensure
survival
in
complex
habitats,
potentially
representing
bioactive
compounds
of
biotechnological
interest.
Pseudarthrobacter
sp.
So.54
is
an
bacteria
strain
isolated
from
the
rhizospheric
soil
Colobanthus
quitensis.
Our
work
aimed
study
its
metabolic
potential,
linked
environmental
adaptation
production
secondary
metabolites
with
possible
applications.
Whole-genome
sequencing,
assembly,
phylogenetic
analysis,
functional
annotation,
islands
prediction
were
performed
determine
taxonomic
affiliation
differential
So.54.
Additionally,
Biosynthetic
Gene
Clusters
(BGCs)
responsible
for
identified.
The
assembled
genome
has
3,871,805
bp
66.0%
G
+
C
content.
Phylogenetic
analysis
confirmed
that
belongs
genus;
nevertheless,
nucleotide
amino
acid
identity
values
below
species
threshold.
main
pathways
64
associated
stress
defense
adaptation,
such
as
heavy
metal
resistance
genes,
AntiSMASH
predicted
six
BGCs
low
or
no
similarity
known
clusters,
suggesting
potential
novel
natural
products.
These
findings
indicate
could
be
a
significant
potential.
Language: Английский
Isolation and characterization of plant growth promoting rhizobacteria (PGPR) from rhizosphere of Helianthus annuus L.
International Journal of Agriculture Environment and Food Sciences,
Journal Year:
2024,
Volume and Issue:
8(2), P. 412 - 429
Published: June 27, 2024
Plant
growth-promoting
rhizobacteria
(PGPR)
support
plant
growth
through
direct
and
indirect
mechanisms.
To
investigate
PGPR
strains
that
growth,
21
bacterial
isolates,
mostly
Bacillus
ssp.
Pseudomonas
ssp.,
were
isolated
from
different
rhizospheric
soils
of
sunflowers
in
Kırşehir
districts
2020.
All
isolates
characterized
morphologically,
biochemically
by
screening
under
vitro
conditions
for
properties
such
as
nitrogen
fixation,
IAA
(indoleacetic
acid)
production,
siderophore
HCN
(hydrogen
cyanide)
inorganic
phosphate
solubility.
It
was
also
screened
extracellular
enzyme
production
antifungal
activity
against
Fusarium
oxysporum.
Among
the
3
(MH-35-4,
MH-49-4,
MH-64-3)
fixed
nitrogen,
2
(MH-59-6,
MH-64-3),
produced
siderophores,
8
MH-35-6,
MH-54-3,
MH-54-4,
MH-59-1,
MH-59-2,
MH-59-4,
MH-59-8)
HCN,
6
(MH-35-6,
IAA,
7
MH-59-8,
solubilized
phosphate.
Additionally,
only
(MH-54-3,
MH-54-4)
positive
amylase
tests,
MH-59-6,
MH-59-7,
citrate
(MH-35-1,
MH-35-4,
MH-35-7,
protease
MH-35-3,
MH-59-7)
gelatin
hydrolysis
tests.
38%
determined
hydrogen
cyanide
producers,
10%
29%
33%
solubilizers
14%
fixers.
Isolate
MH-35-6
showed
highest
oxysporum
with
an
inhibition
rate
53.57%.
This
followed
MH-54-1
(51.19%),
MH-54-3
(47.61%)
MH-59-2
(38.09%),
respectively.
Therefore,
our
study
reveals
bacteria
promote
can
be
used
to
increase
crop
yield
a
biocontrol
agent.
Language: Английский