Berberine-inspired ionizable lipid for self-structure stabilization and potent brain targeting delivery of nucleic acid therapeutics DOI
Zhi‐Hong Jiang, Chong Li,

Xufei Bian

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Abstract Despite advancements in targeting organs such as the liver, spleen, and lungs with lipid nanoparticles (LNPs), challenge of traversing blood-brain barrier (BBB) significantly impedes progress gene therapies for neurological disorders. Motivated by structural functional characteristics alkaloids, we developed a novel library ionizable molecules based on tetrahydroisoquinoline structure characteristic protoberberine family. Our findings reveal that: (i) LNPs incorporating berberine-derived lipids notably enhance ability to cross BBB, increasing in vitro endocytosis efficiency up 65-fold achieving an vivo brain-to-liver distribution ratio approaching 20%; (ii) these form stable self-assemblies polyA, enhancing nucleic acid stability through mechanisms beyond conventional electrostatic interactions, thus providing effective RNA protection without need additional modifications; (iii) inherit diverse brain-protective properties protoberberine-type including anti-inflammatory antioxidant effects, thereby synergistically therapeutic management brain diseases while exhibiting minimal immunogenicity.

Language: Английский

Covalent conjugation of pumpkin seed protein induction by epigallocatechin-3-gallate: Structural formation, functional and in vitro digestion properties DOI Creative Commons

Yongqiang Xu,

Menglin Guo,

Chenyu Zhang

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117636 - 117636

Published: March 1, 2025

Language: Английский

Citations

2
Neurodegenerative diseases and catechins: (−)-epigallocatechin-3-gallate is a modulator of chronic neuroinflammation and oxidative stress DOI Creative Commons
Siying Li,

Zaoyi Wang,

Gang Liu

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Aug. 1, 2024

Catechins, a class of phytochemicals found in various fruits and tea leaves, have garnered attention for their diverse health-promoting properties, including potential combating neurodegenerative diseases. Among these catechins, (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol green tea, has emerged as promising therapeutic agent due to its potent antioxidant anti-inflammatory effects. Chronic neuroinflammation oxidative stress are key pathological mechanisms diseases such Alzheimer's disease (AD) Parkinson's (PD). EGCG neuroprotective efficacy scavenging free radicals, reducing attenuating neuroinflammatory processes. This review discusses molecular EGCG's anti-oxidative chronic neuroinflammation, emphasizing effects on autoimmune responses, neuroimmune system interactions, focusing related AD PD. By elucidating action impact processes, this underscores intervention AD, PD, possibly other Overall, emerges natural compound stress, offering novel avenues strategies treatment disorders.

Language: Английский

Citations

10
Metal-phenolic nanoparticles enhance low temperature photothermal therapy for bacterial biofilm in superficial infections DOI Creative Commons

Yang Ye,

Qinqin Zheng,

Ziqi Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 15, 2024

Bacterial infections, especially induced by multidrug-resistant pathogens, have become a significant global health concern. In the infected tissues, biofilms not only serve as source of nutrients but also act protective barriers that impede antibiotic penetration. Herein, we developed tea polyphenols epigallocatechin gallate (EGCG) Au nanoparticles (E-Au NPs) through direct one-step self-assembly methods EGCG chelating with ions to eradicate antibiotic-resistant bacteria methicillin-resistant Staphylococcus aureus (MRSA) and prevent formation biofilm under near-infrared (NIR) irradiation. The outstanding antibacterial effect involved in mild photothermal therapy, reactive oxygen species production, pathogenicity-related genes regulation, quinoprotein were specific polyphenol-based NPs. excellent anti-inflammatory therapeutic efficacy E-Au NPs was validated topically applied murine MRSA-infected skin wounds keratitis model vivo kill bacteria, reduce inflammation response promote wound healing. Furthermore, ophthalmic systemic biosafety profiles thoroughly evaluated while no side effects revealed achieving balance between high-efficiency properties biocompatibility. This study provides an effective agent metal-phenolic materials for superficial tissue infection favorable prognosis potential clinical translation.

Language: Английский

Citations

7
Extraction of phenolic compounds from lucuma (Pouteria lucuma) seeds with natural deep eutectic solvents: modelling using response surface methodology and artificial neural networks DOI Creative Commons
Gustavo Puma-Isuiza, Juliana María García-Chacón, Coralia Osorio

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 8

Published: June 5, 2024

The present study aimed to extract polyphenolic compounds from lucuma ( Pouteria ) seeds using natural deep eutectic solvents (NADES) as a green, efficient, and environmentally friendly extraction. This was optimized by the Response Surface Method (RSM) comparing its predictive capacity with Artificial Neural Networks (ANN) Convolutional (CNN). Four NADES were prepared mixing lactic acid (LA) each of following reagents: sodium acetate (SA), urea (U), glucose (G), ammonium (AA), separately. yield total phenolic (TPC) obtained measured an optimization criterion Box-Benhken design. factors evaluated: time, temperature, seed flour (LSF): ratio. response variables TPC antioxidant activity. LA-AA selected because it exhibited highest value analyzed UHPLC–MS (Ultra-performance Liquid Chromatography-Mass Spectrometry). From RSM, optimal extraction parameters 80 min, 52°C, LSF: ratio 8:100 (w/v), obtaining 3601.51 ± 0.51 mg GAE/100 g LFS. analysis evidenced formation epigallocatechin isomers gallate. ability ANNs compared RSM demonstrated.

Language: Английский

Citations

5
Effects of Green Tea Extract Epigallocatechin-3-Gallate on Oral Diseases: A Narrative Review DOI Creative Commons
Yizhen Li, Lei Cheng, Mingyun Li

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(8), P. 634 - 634

Published: July 29, 2024

Oral diseases are among the most prevalent globally. Accumulating new evidence suggests considerable benefits of epigallocatechin-3-gallate (EGCG) for oral health. This review aims to explore role and application EGCG in main diseases.

Language: Английский

Citations

5
Production of protein-epigallocatechin gallate conjugates using free radicals induced by ultrasound and their gelation behavior DOI
Chen Li,

Yalu Zheng,

Xiaohui Xiong

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141300 - 141300

Published: Sept. 19, 2024

Language: Английский

Citations

5
Epigallocatechin-3-gallate therapeutic potential in human diseases: molecular mechanisms and clinical studies DOI Creative Commons
Manzar Alam,

Mehak Gulzar,

Salman Akhtar

et al.

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Dec. 27, 2024

Abstract Green tea has garnered increasing attention across age groups due to its numerous health benefits, largely attributed Epigallocatechin 3-gallate (EGCG), key polyphenol. EGCG exhibits a wide spectrum of biological activities, including antioxidant, anti-inflammatory, antibacterial, anticancer, and neuroprotective properties, as well benefits for cardiovascular oral health. This review provides comprehensive overview recent findings on the therapeutic potential in various human diseases. Neuroprotective effects include safeguarding neurons from damage enhancing cognitive function, primarily through antioxidant capacity reduce reactive oxygen species (ROS) generated during physiological stress. Additionally, modulates signaling pathways such JAK/STAT, Delta-Notch, TNF, all which play critical roles neuronal survival, growth, function. Furthermore, is involved regulating apoptosis cell cycle progression, making it promising candidate treatment metabolic diseases, cancer diabetes. Despite potential, further clinical trials are essential validate efficacy safety optimize delivery target tissues. While many reviews have addressed anticancer properties EGCG, this focuses molecular mechanisms by used specific particularly cancer, neurodegenerative It serves valuable resource researchers, clinicians, healthcare professionals, revealing managing disorders, diseases highlighting broader values.

Language: Английский

Citations

5
Green Tea Polyphenol Epigallocatechin Gallate Interactions with Copper-Serum Albumin DOI Creative Commons
Meiling Fu, Liangliang Zhang,

Rick Killeen

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 320 - 320

Published: Jan. 15, 2025

Epigallocatechin gallate (EGCg), an abundant phytochemical in green tea, is antioxidant that also binds proteins and complex metals. After gastrointestinal absorption, EGCg to serum albumin the hydrophobic pocket between domains IIA IIIA overlaps with Sudlow I site. Serum has two metal binding sites, a high-affinity N-terminal site (NTS) selectively Cu(II), low-affinity, less selective multi-metal (MBS). We proposed determine whether or reacts Cu(II)-serum using fluorescence, UV–Visible electron paramagnetic resonance (EPR) spectroscopy. Our results suggest when loaded Cu(II) both MBS-Cu(II) reduces copper Cu(I). does not bind react NTS Potential consequences include changes homeostasis damage from pro-oxidative Fenton reactions.

Language: Английский

Citations

0
Exploring the impact of cationic polysaccharide concentration on the formation of high internal phase emulsion based on egg white proteins: A study on emulsion interfacial structure and co-delivery of epigallocatechin gallate/β-carotene DOI

Jingbo Liu,

Longxiang Li, Shanglin Li

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111250 - 111250

Published: Feb. 1, 2025

Language: Английский

Citations

0
Neuroprotective insights into epigallocatechin gallate (EGCG) for neurodegenerative disorders DOI Creative Commons
Neha Kamboj,

S.D. Sharma,

Rahul Kumar

et al.

Exploration of neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

Neurodegenerative disorders, including Alzheimer’s, Parkinson’s, Huntington’s, and amyotrophic lateral sclerosis, are among the most significant health concerns worldwide, characterized by neuronal dysfunction, oxidative stress, neuroinflammation, protein misfolding. Epigallocatechin gallate, a green tea polyphenol, has been reported to possess multifaceted neuroprotective properties. It reduces stress through free radical scavenging, activation of antioxidant enzymes, stabilization mitochondrial function. also inhibits neuroinflammation modulation key signaling pathways. suppresses amyloid-beta aggregation in Alzheimer’s alpha-synuclein fibrillation thus attenuating toxic accumulation. Its activity induction autophagy promotion synaptic plasticity supports survival However, low bioavailability metabolic instability hinder its translation into clinic. Strategies nanoparticle encapsulation, structural modifications, combination therapies being explored overcome these challenges. Future research could establish epigallocatechin gallate as viable candidate for managing neurodegenerative disorders.

Language: Английский

Citations

0