Baicalein suppresses inflammation and attenuates acute lung injury by inhibiting glycolysis via HIF‑1α signaling DOI Creative Commons
Zixiang Liu,

Xiaona Zheng,

Ning Li

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 5, 2024

Baicalein, a flavonoid monomer compound isolated from the dried root of traditional Chinese herb Scutellaria baicalensis, has several pharmacological activities, such as anti‑inflammatory, anti‑angiogenic, antitumor, antimicrobial and antiviral properties. Acute lung injury (ALI) is characterized by alveolar epithelium capillary endothelium, which results in decreased volume, compliance, ventilation/perfusion mismatch, intrapulmonary edema, edema even acute hypoxemic respiratory failure. The present study aimed to investigate effects baicalein on inflammation. Bioinformatics analysis using network pharmacology predicted that hypoxia inducible factor‑1α (HIF‑1α) glycolysis signaling pathways were involved mechanism underlying therapeutic baicalein. Further in vitro vivo experiments, immunohistochemistry, immunofluorescence PCR, verified could inhibit HIF‑1α signaling, thus suppressing glycolysis, improving inflammatory responses ALI. Taken together, suggested anti‑inflammatory treating ALI associated with its ability suppress via pathway.

Language: Английский

Exploring treatment options in cancer: Tumor treatment strategies DOI Creative Commons
Beilei Liu, Hongyu Zhou, Licheng Tan

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 17, 2024

Traditional therapeutic approaches such as chemotherapy and radiation therapy have burdened cancer patients with onerous physical psychological challenges. Encouragingly, the landscape of tumor treatment has undergone a comprehensive remarkable transformation. Emerging fervently pursued modalities are small molecule targeted agents, antibody-drug conjugates (ADCs), cell-based therapies, gene therapy. These cutting-edge not only afford personalized precise targeting, but also provide enhanced comfort potential to impede disease progression. Nonetheless, it is acknowledged that these strategies still harbour untapped for further advancement. Gaining understanding merits limitations holds promise offering novel perspectives clinical practice foundational research endeavours. In this review, we discussed different modalities, including drugs, peptide antibody cell therapy, It will detailed explanation each method, addressing their status development, challenges, solutions. The aim assist clinicians researchers in gaining deeper diverse options, enabling them carry out effective advance more efficiently.

Language: Английский

Citations

219
Aspalathin-Rich Unfermented Aspalathus linearsis Extract Mitigates Vascular Endothelial Inflammation by Inhibiting the TNF-α/Hexokinase 2/NF-κB Signaling Pathway DOI Creative Commons

Ya‐Pei Wang,

Bi-Juan Wang,

Hsin-Ying Lu

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101882 - 101882

Published: April 1, 2025

Language: Английский

Citations

1
Immunometabolic reprogramming of macrophages with inhalable CRISPR/Cas9 nanotherapeutics for acute lung injury intervention DOI

Wanling Huang,

Gaohong Fu,

Yangeng Wang

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 181, P. 308 - 316

Published: April 1, 2024

Language: Английский

Citations

5
High-intensity interval training and medium-intensity continuous training may affect cognitive function through regulation of intestinal microbial composition and its metabolite LPS by the gut-brain axis DOI

Mei Peng,

Ruihan Zou,

Sisi Yao

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 352, P. 122871 - 122871

Published: June 25, 2024

Language: Английский

Citations

5
The impact of glycolysis on ischemic stroke: from molecular mechanisms to clinical applications DOI Creative Commons
Yingquan Liu,

Peijia Hu,

Hongliang Cheng

et al.

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Ischemic stroke (IS), a leading cause of disability and mortality worldwide, remains significant challenge due to its complex pathogenesis. Glycolysis, central metabolic pathway, plays critical role in bridging the gap between dysfunction neurological impairment. During ischemic conditions, glycolysis replaces oxidative phosphorylation as primary energy source for brain tissue. However, ischemia–reperfusion state, neuronal cells show particular reliance on aerobic glycolysis. Immune cells, such monocytes, also contribute atheromatous plaque formation thrombi through increased Given glycolysis’s involvement various pathological stages IS, it offers potential improved diagnosis, treatment, prevention. This review comprehensively explores different phases addresses existing controversies, discusses diagnostic therapeutic applications. By elucidating intricate relationship this aims provide novel insights future research clinical advancements.

Language: Английский

Citations

0
Antibiotic-derived approaches in cancer therapy: effectiveness of ikarugamycin in hexokinase-2 inhibition, tissue factor modulation, and metabolic regulation in breast cancer DOI

Serra Vildan Akgul Obeidin,

Mustafa Şenol,

Zikret Köseoğlu

et al.

Anti-Cancer Drugs, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

We aimed to explore the role of ikarugamycin (IKA) in breast cancer, its connection with hexokinase-2 (HK-2) repression, and tissue factor (TF). This study sought extend HK-2 as a TF activator comprehensive analysis these interactions from enzyme, gene, protein levels. The investigation was performed MDA-MB-231 MCF-7 cancer lines. oxidative stress index (OSI), lactate production, HK activity were assessed using colorimetric assays. Western blot quantitative PCR analyses determine expressions. Prothrombin time Tests additionally effect IKA therapy on activation. Three over four significantly downregulated genes identified after specific IKA's cell In treatment group, production markedly reduced (P < 0.05) hexokinase found be all groups 0.05, <0.01). Paclitaxel cytotoxicity independently causes lower OSI IKA-treated compared controls even though is elevated control. Molecular results demonstrated expressions at level P 0.01). Partial thromboplastin also showed that cells had longer activation duration. A potential indirect association inhibition regulation put forward this by presenting bioactivation protein, enzyme

Language: Английский

Citations

0
Cirsium japonicum leaf extract attenuated lipopolysaccharide-induced acute respiratory distress syndrome in mice via suppression of the NLRP3 and HIF1α pathways DOI
Eun Yeong Lim,

Gun‐Dong Kim,

Ha‐Jung Kim

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156601 - 156601

Published: March 2, 2025

Language: Английский

Citations

0
Transcriptional and neuroprotective effects of hexokinase-2 inhibitors administered after stroke DOI
Seok Joon Won, Gӧkhan Uruk, Nguyen Mai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT The inflammatory response induced by stroke can exacerbate injury to peri-infarct tissue. Microglia and other immune cells that mediate this require increased glycolytic flux during pro-inflammatory activation. These cells, unlike neurons most cell types, utilize hexokinase-2 (HK2) rather than hexokinase-1 for glycolysis, such HK2 inhibitors may selectively target them suppress post-ischemic inflammation. Here we compared the effects of non-selective hexokinase inhibitor 2-deoxyglucose HK2-selective lonidamine 3-bromopyruvate on secondary after stroke. A spatial transcriptomic assessment was performed in parallel compare microglial gene expression microglia - neuron interactions screen off-target effects. Each suppressed upregulation attenuated stress functional pathways neighboring neurons, but had minimal effect neuronal uninjured cortex. were more effective suppressing morphological changes, oxidative stress, neurite loss. administered produced long-term improvements outcome. Selective thus provide a clinically applicable means activation thereby improve outcomes without endangering energy metabolism.

Language: Английский

Citations

0
Methylglyoxal is an antibacterial effector produced by macrophages during infection DOI Open Access
Andrea Anaya-Sanchez, Samuel B. Berry,

Scott Espich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Infected macrophages transition into aerobic glycolysis, a metabolic program crucial for control of bacterial infection. However, antimicrobial mechanisms supported by glycolysis are unclear. Methylglyoxal is highly toxic aldehyde that modifies proteins and DNA produced as side-product glycolysis. Here we show despite the toxicity this aldehyde, infected generate high levels methylglyoxal during while downregulating detoxification system. We use targeted mutations in mice to modulate generation reducing production host promotes survival Listeria monocytogenes Mycobacterium tuberculosis , whereas increasing improves Furthermore, bacteria unable detoxify avirulent experience up 1000-fold greater genomic mutation frequency Taken together, these results suggest an innate immune effector defends against pathogens.

Language: Английский

Citations

0
Baicalein suppresses inflammation and attenuates acute lung injury by inhibiting glycolysis via HIF‑1α signaling DOI Creative Commons
Zixiang Liu,

Xiaona Zheng,

Ning Li

et al.

Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 5, 2024

Baicalein, a flavonoid monomer compound isolated from the dried root of traditional Chinese herb Scutellaria baicalensis, has several pharmacological activities, such as anti‑inflammatory, anti‑angiogenic, antitumor, antimicrobial and antiviral properties. Acute lung injury (ALI) is characterized by alveolar epithelium capillary endothelium, which results in decreased volume, compliance, ventilation/perfusion mismatch, intrapulmonary edema, edema even acute hypoxemic respiratory failure. The present study aimed to investigate effects baicalein on inflammation. Bioinformatics analysis using network pharmacology predicted that hypoxia inducible factor‑1α (HIF‑1α) glycolysis signaling pathways were involved mechanism underlying therapeutic baicalein. Further in vitro vivo experiments, immunohistochemistry, immunofluorescence PCR, verified could inhibit HIF‑1α signaling, thus suppressing glycolysis, improving inflammatory responses ALI. Taken together, suggested anti‑inflammatory treating ALI associated with its ability suppress via pathway.

Language: Английский

Citations

0