Kaempferol-3-O-rutinoside protects myocardial cell injury by inhibiting the TXNIP/NLRP3 pathway DOI Creative Commons
Lingli Shi,

Xiao-ni Zhao,

Juan Bai

et al.

Italian Journal of Food Science, Journal Year: 2024, Volume and Issue: 36(3), P. 291 - 300

Published: Aug. 13, 2024

Kaempferol-3-O-rutinoside (KR), a compound commonly found in green tea, has demonstrated significant myocardial protective effects. The aim of this study was to reveal the cardioprotective mechanism KR. In study, molecular docking employed predict binding affinity KR thioredoxin-interacting protein (TXNIP). An injury model H9c2 cells established using lipopolysaccharide (LPS) and adenosine triphosphate (ATP). Lactate dehydrogenase (LDH) levels were measured specific kits, while total superoxide dismutase (T-SOD), malondialdehyde (MDA), glutathione (GSH), catalase (CAT) activities assessed with colorimetric assays. reactive oxygen species (ROS) level determined DCFH-DA fluorescent probe assay. addition, expression TXNIP, NLR-family pyrin domain-containing 3 (NLRP3), cysteinyl aspartate proteinase-1 (Caspase-1), thioredoxin (TRX) quantified by reverse transcription polymerase chain reaction (RT-PCR) Western blot (WB) Levels interleukin-1β (IL-1β) IL-18 ELISA. results indicated that for TXNIP. reduce LDH MDA activities, increase CAT, GSH, T-SOD, inhibit ROS production. Mechanistically, decreased gene expressions Caspase-1, NLRP3, increasing TRX. Also, IL-1β IL-18. conclusion, against cardiomyocyte involves inhibition TXNIP/NLRP3 pathway, providing experimental evidence its potential clinical application.

Language: Английский

Exogenous hydrogen sulfide improves non-alcoholic fatty liver disease by inhibiting endoplasmic reticulum stress/NLRP3 inflammasome pathway DOI

Xiaodi Fu,

Qi Zhang, Yuhang Chen

et al.

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Language: Английский

Citations

1
Emerging insights into the role of NLRP3 inflammasome and endoplasmic reticulum stress in renal diseases DOI

Yanting Zhang,

Shiyun Guo,

Xiaodi Fu

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 136, P. 112342 - 112342

Published: May 30, 2024

Language: Английский

Citations

8
The Role of Hydrogen Sulfide in the Regulation of the Pulmonary Vasculature in Health and Disease DOI Creative Commons
Philip I. Aaronson

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 341 - 341

Published: March 14, 2025

The gasotransmitter hydrogen sulfide (H2S; also termed sulfide) generally acts as a vasodilator in the systemic vasculature but causes paradoxical constriction of pulmonary arteries (PAs). In light evidence that fall partial pressure oxygen (pO2) increases cellular levels, it was proposed rise artery smooth muscle cells (PASMCs) is responsible for hypoxic vasoconstriction, contraction PAs which develops rapidly lung regions undergoing alveolar hypoxia. contrast, hypertension (PH), sustained elevation (PAP) can develop presence diverse array pathological stimuli, including chronic hypoxia, associated with decrease expression -producing enzymes PASMCs and corresponding production by lung. Evidence PAP animal models PH be lowered administration exogenous has led to an interest using sulfide-donating agents treating this condition humans. Notably, intracellular H2S exists equilibrium other sulfur-containing species such polysulfides persulfides, these reactive sulfur are thought mediate most its effects on through persulfidation cysteine thiols proteins, leading changes function manner similar thiol oxidation species. This review sets out what currently known about mechanisms related exert their actions vascular tone, both acutely chronically, discusses potential sulfide-releasing drugs treatments different types arise

Language: Английский

Citations

0
Oral microbiota and respiratory diseases: advances and perspectives DOI
X Y Liu,

Fengxiang Shi,

Jiawei Zeng

et al.

Clinical Microbiology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

SUMMARYThe oral microbiota, characterized by its complexity and density, is increasingly recognized for significant association with respiratory diseases their pathogenesis. Changes in the microbiome, including shifts relative abundance of certain harmful microbes, byproducts, virulence elements, have been linked to disease development progression. The use microbiome indicators treatments essential detection, prognosis, management illnesses, providing practical benefits. Hence, a thorough understanding correlation between microbiota illnesses imperative generating novel therapeutic approaches rooted address these ailments. This review summarizes how are connected diseases, explores mechanisms influence, discusses treatment approaches.

Language: Английский

Citations

0
Cr(VI) induced hepatocyte apoptosis through the CTH/H2S/Drp1 signaling pathway DOI
Jie Zhou, Xin Zheng, Xi Chen

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175332 - 175332

Published: Aug. 6, 2024

Language: Английский

Citations

2
Advancements in increasing efficiency of Hydrogen Sulfide in therapeutics: Strategies for targeted delivery as prodrugs DOI
Tejasvi Pandey, Vivek Pandey

Nitric Oxide, Journal Year: 2024, Volume and Issue: 152, P. 1 - 10

Published: Sept. 6, 2024

Language: Английский

Citations

1
Hydrogen sulfide plays an important role by regulating microRNA in different ischemia–reperfusion injury DOI
Qi Zhang,

Yanting Zhang,

Shiyun Guo

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 229, P. 116503 - 116503

Published: Aug. 23, 2024

Language: Английский

Citations

1
Kaempferol-3-O-rutinoside protects myocardial cell injury by inhibiting the TXNIP/NLRP3 pathway DOI Creative Commons
Lingli Shi,

Xiao-ni Zhao,

Juan Bai

et al.

Italian Journal of Food Science, Journal Year: 2024, Volume and Issue: 36(3), P. 291 - 300

Published: Aug. 13, 2024

Kaempferol-3-O-rutinoside (KR), a compound commonly found in green tea, has demonstrated significant myocardial protective effects. The aim of this study was to reveal the cardioprotective mechanism KR. In study, molecular docking employed predict binding affinity KR thioredoxin-interacting protein (TXNIP). An injury model H9c2 cells established using lipopolysaccharide (LPS) and adenosine triphosphate (ATP). Lactate dehydrogenase (LDH) levels were measured specific kits, while total superoxide dismutase (T-SOD), malondialdehyde (MDA), glutathione (GSH), catalase (CAT) activities assessed with colorimetric assays. reactive oxygen species (ROS) level determined DCFH-DA fluorescent probe assay. addition, expression TXNIP, NLR-family pyrin domain-containing 3 (NLRP3), cysteinyl aspartate proteinase-1 (Caspase-1), thioredoxin (TRX) quantified by reverse transcription polymerase chain reaction (RT-PCR) Western blot (WB) Levels interleukin-1β (IL-1β) IL-18 ELISA. results indicated that for TXNIP. reduce LDH MDA activities, increase CAT, GSH, T-SOD, inhibit ROS production. Mechanistically, decreased gene expressions Caspase-1, NLRP3, increasing TRX. Also, IL-1β IL-18. conclusion, against cardiomyocyte involves inhibition TXNIP/NLRP3 pathway, providing experimental evidence its potential clinical application.

Language: Английский

Citations

0