D-Serine Can Modify the Wall Teichoic Acid of MRSA via the dlt Pathway DOI Open Access
Lei Wang, Jing Xie, Qing Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4110 - 4110

Published: April 25, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) infection is a serious clinical threat, and D-Serine (D-Ser) showed significant sensitization effects on β-lactams against MRSA in our previous study. Quantitative PCR analysis found the elevated expression of dlt operon with D-Ser combination, which responsible for wall teichoic acid (WTA) modification involving D-Alanine (D-Ala). This study aims to verify effect WTA through pathway explore related bacteria. The DltA DltC were recombined, enzyme kinetic evaluations different D-amino acids then conducted; it was that second-best substrate (just after D-Ala), no matter whether present or not. treatment also lowered generation as demonstrated by phosphate quantification native-PAGE electrophoresis, increased susceptibility S. polymyxins, mouse survival rate intraperitoneal model without affecting bacterial loads main organs, indicating possible virulence modification. In conclusion, current provided evidence via pathway, its involvement deserves further investigation.

Language: Английский

Discovery, total synthesis, and biological evaluation of tyrcinnamins as antibacterial agents and tyrosinase activators DOI
Di Mao, B. Wei, Chao Liu

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 117665 - 117665

Published: April 1, 2025

Language: Английский

Citations

0
D-Serine Can Modify the Wall Teichoic Acid of MRSA via the dlt Pathway DOI Open Access
Lei Wang, Jing Xie, Qing Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4110 - 4110

Published: April 25, 2025

Methicillin-resistant Staphylococcus aureus (MRSA) infection is a serious clinical threat, and D-Serine (D-Ser) showed significant sensitization effects on β-lactams against MRSA in our previous study. Quantitative PCR analysis found the elevated expression of dlt operon with D-Ser combination, which responsible for wall teichoic acid (WTA) modification involving D-Alanine (D-Ala). This study aims to verify effect WTA through pathway explore related bacteria. The DltA DltC were recombined, enzyme kinetic evaluations different D-amino acids then conducted; it was that second-best substrate (just after D-Ala), no matter whether present or not. treatment also lowered generation as demonstrated by phosphate quantification native-PAGE electrophoresis, increased susceptibility S. polymyxins, mouse survival rate intraperitoneal model without affecting bacterial loads main organs, indicating possible virulence modification. In conclusion, current provided evidence via pathway, its involvement deserves further investigation.

Language: Английский

Citations

0