Down-regulated circ_0001853 inhibits lipopolysaccharide-induced endometritis progression via sponging miR-34c-5p DOI Creative Commons
Qian Xu, Peng An, Liyun Zhao

et al.

Molecular and Cellular Probes, Journal Year: 2025, Volume and Issue: unknown, P. 102023 - 102023

Published: March 1, 2025

To investigate the diagnostic value and mechanism of action circular RNA (circ_) circ_0001853 microRNA (miR) miR-34c-5p in patients with endometritis. 189 endometritis 176 healthy individuals were included this study. Venous blood serum was collected from study subjects stored temporarily at -80°C. Real-time quantitative chain polymerase reaction (RT-qPCR) used to detect expression, receiver operating characteristic (ROC) curves assessed both predicting Cell counting kit (CCK8) observed cell proliferation, flow cytometry recorded apoptosis, enzyme linked immunosorbent assay (ELISA) monitored inflammatory factor dual luciferase reporter immunoprecipitation (RIP) verified relationship between targeting interactions. High levels low present patients, they negatively correlated. Both alone or combination had progression Transfection si-circ_0001853 promoted proliferation reduced apoptosis cellular inflammation induced by lipopolysaccharide (LPS) stimulation. There a direct reciprocal circ_0001853, use inhibitor resisted silencing increased number apoptotic cells levels. is involved through miR-34c-5p, i.e., promotes miR-34c-5p-induced epithelial cells, reduces suppresses levels, preventing disease progression.

Language: Английский

Down-regulated circ_0001853 inhibits lipopolysaccharide-induced endometritis progression via sponging miR-34c-5p DOI Creative Commons
Qian Xu, Peng An, Liyun Zhao

et al.

Molecular and Cellular Probes, Journal Year: 2025, Volume and Issue: unknown, P. 102023 - 102023

Published: March 1, 2025

To investigate the diagnostic value and mechanism of action circular RNA (circ_) circ_0001853 microRNA (miR) miR-34c-5p in patients with endometritis. 189 endometritis 176 healthy individuals were included this study. Venous blood serum was collected from study subjects stored temporarily at -80°C. Real-time quantitative chain polymerase reaction (RT-qPCR) used to detect expression, receiver operating characteristic (ROC) curves assessed both predicting Cell counting kit (CCK8) observed cell proliferation, flow cytometry recorded apoptosis, enzyme linked immunosorbent assay (ELISA) monitored inflammatory factor dual luciferase reporter immunoprecipitation (RIP) verified relationship between targeting interactions. High levels low present patients, they negatively correlated. Both alone or combination had progression Transfection si-circ_0001853 promoted proliferation reduced apoptosis cellular inflammation induced by lipopolysaccharide (LPS) stimulation. There a direct reciprocal circ_0001853, use inhibitor resisted silencing increased number apoptotic cells levels. is involved through miR-34c-5p, i.e., promotes miR-34c-5p-induced epithelial cells, reduces suppresses levels, preventing disease progression.

Language: Английский

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