Published: Jan. 1, 2024
Language: Английский
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: March 18, 2024
The global challenge posed by cancer, marked rising incidence and mortality rates, underscores the urgency for innovative therapeutic approaches. PI3K/Akt signaling pathway, frequently amplified in various cancers, is central regulating essential cellular processes. Its dysregulation, often stemming from genetic mutations, significantly contributes to cancer initiation, progression, resistance therapy. Concurrently, ferroptosis, a recently discovered form of regulated cell death characterized iron-dependent processes lipid reactive oxygen species buildup, holds implications diseases, including cancer. Exploring interplay between dysregulated pathway ferroptosis unveils potential insights into molecular mechanisms driving or inhibiting ferroptotic cells. Evidence suggests that may sensitize cells induction, offering promising strategy overcome drug resistance. This review aims provide comprehensive exploration this interplay, shedding light on disrupting enhance as an alternative route inducing improving treatment outcomes.
Language: Английский
Citations
28
Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)
Published: May 29, 2024
Abstract Background Sodium-glucose transporter 2 (SGLT2) inhibitors (iSGLT2) are approved medications for type diabetes. Recent studies indicate that iSGLT2 inhibit the growth of some cancer cells. However, mechanism(s) remains to be fully elucidated. Methods The SGLT2 levels were determined in normal colon CCD 841 CoN and, HCT 116, HT-29, SW480 and LoVo colorectal (CRC) cell lines by quantitative real-time PCR western blot. effect canagliflozin on proliferation was examined using CCK-8, as its role CRC cells metabolism tumorigenesis has been evaluated XF HS Seahorse Bioanalyzer flow cytometric analyses. Transient gene silencing experiments analysis protein–protein interaction network conducted evaluate molecular targets Results Data showed treatment with (50 µM) 72 h induced cycle arrest ( p < 0.001), impaired glucose energetic promoted apoptotic death ER stress flowing into autophagy 0.001) 116 HT-29 These cellular events accompanied sirtuin 3 (SIRT3) upregulation 0.01), also supported SIRT3 transient resulting attenuation effects metabolic/energetic alterations induction programmed death. identification validation dipeptidyl peptidase 4 (DPP4) potential common target assessed. Conclusions results deepened knowledge contribution limiting unveiling SGLT2/SIRT3 axis cytotoxic mechanisms. Graphical
Language: Английский
Citations
9
Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1420 - 1420
Published: Aug. 25, 2024
Aging and comorbidities like type 2 diabetes obesity contribute to the development of chronic systemic inflammation, which impacts heart failure vascular disease. Increasing evidence suggests a role pro-inflammatory M1 macrophages in inflammation. A shift metabolism from mitochondrial oxidation glycolysis is essential for activation phenotype. Thus, reprogramming macrophage may alleviate phenotype protect against cardiovascular diseases. In present study, we hypothesized that estrogen receptors leads elevation deacetylase Sirt3, supports function mitigates macrophages.
Language: Английский
Citations
3
Prostaglandins Leukotrienes and Essential Fatty Acids, Journal Year: 2024, Volume and Issue: 203, P. 102652 - 102652
Published: April 1, 2024
Language: Английский
Citations
2
Chemical Biology & Drug Design, Journal Year: 2024, Volume and Issue: 103(2)
Published: Jan. 29, 2024
Abstract Since the discovery of sirtuin family founding member (i.e., yeast silent information regulator 2 (sir2) protein) in 2000, more and proteins have been identified are currently known to be present organisms from all three kingdoms life bacteria, archaea, eukarya). Seven mammals including humans, that is, SIRT1/2/3/4/5/6/7. Sirtuin a class enzymes with primary catalytic activity being β‐nicotinamide adenine dinucleotide (β‐NAD + or NAD )‐dependent deacylation N ε ‐acyl‐lysine residues on cellular proteins. Many sirtuins (e.g., human SIRT1/2/3/4/5/6/7) found each possess multiple individual deacylase activities acting substrates different acyl groups ranging simple formyl acetyl complex like succinyl myristoyl; however, our current knowledge (patho)physiological roles these is still limited, which could due thin research toolbox for investigation deacylase‐selective mutant inhibitor/activator). In this article, an updated account subject matter will presented biochemical medicinal chemistry perspectives.
Language: Английский
Citations
1
Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(6), P. 810 - 810
Published: June 20, 2024
Cancer cells modulate their metabolism, creating an acidic microenvironment that, in turn, can favor tumor progression and chemotherapy resistance. Tumor adopt strategies to survive a drop extracellular pH (pHe). In the present manuscript, we investigated contribution of mitochondrial sirtuin 3 (SIRT3) adaptation survival cancer low pHe. SIRT3-overexpressing silenced breast MDA-MB-231 human embryonic kidney HEK293 were grown buffered unbuffered media at 7.4 6.8 for different times. mRNA expression SIRT3 CAVB, was measured by RT-PCR. Protein SIRT3, CAVB autophagy proteins estimated western blot. SIRT3-CAVB interaction determined immunoprecipitation proximity ligation assays (PLA). Induction studied blot TEM. overexpression increases both cell lines. Moreover, demonstrated that controls intracellular (pHi) through regulation carbonic anhydrase VB (CAVB). Interestingly, obtained similar results using MC2791, new activator. Our point possibility modulating decrease response resistance ameliorate effectiveness anticancer therapy.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0