Differential Effects of Hearing Loss Mutations in Homomeric P2X2 and Heteromeric P2X2/3 Receptors DOI Creative Commons

P. Wand,

Xenia Brünings, Debanjan Tewari

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 510 - 510

Published: March 29, 2025

P2X receptors are unspecific cation channels activated by ATP. They expressed in various tissues and found neuronal immune cells. In mammals, seven subunits described, which can assemble into homomeric heteromeric trimers. P2X2 play important roles cochlear adaptation to elevated sound levels. Three mutations causing inherited progressive hearing loss have been identified. These localize the transmembrane domain 1 (V60L), 2 (G353R) a β-sheet linking ATP binding site pore (D273Y). Herein, were studied human as well P2X2/3 receptors. We measured their of fluorescently labeled derivative (fATP) characterized constructs using patch-clamp technique. The conclusions from our results follows: 1. V60L G353R show robust localization on plasma membrane fATP, whereas mutant D273Y has no fATP. 2. mutation an increased affinity fATP compared with wildtype. 3. expression hP2X2 reduces cell viability, may support its role pathogenesis loss. 4. All distinct phenotypes.

Language: Английский

The Research Progress on the Role of Adenosine A1 Receptors in Acupuncture Analgesia Mechanism DOI Creative Commons

Hong Xu,

Ani Zheng,

Chunyan Zhang

et al.

Journal of Contemporary Medical Practice, Journal Year: 2025, Volume and Issue: 7(2), P. 173 - 175

Published: Feb. 28, 2025

Acupuncture analgesia has become a key focus of acupuncture research in recent years, and the role adenosine receptors acupuncture-induced requires further investigation. Adenosine (ADO) is an important neurotransmitter central nervous system, which exerts different physiological effects by binding to various receptor subtypes (A1, A2A, A2B, A3), playing particularly significant pain regulation analgesia. Among these, highest affinity for A1 (A1R), A1R plays crucial transmission modulation signals. Previous studies have shown that associated with receptor. This study briefly introduces distribution functions receptors, on involvement at both peripheral levels. It aims provide insights into exploration mechanisms systems their

Language: Английский

Citations

0
Differential Effects of Hearing Loss Mutations in Homomeric P2X2 and Heteromeric P2X2/3 Receptors DOI Creative Commons

P. Wand,

Xenia Brünings, Debanjan Tewari

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 510 - 510

Published: March 29, 2025

P2X receptors are unspecific cation channels activated by ATP. They expressed in various tissues and found neuronal immune cells. In mammals, seven subunits described, which can assemble into homomeric heteromeric trimers. P2X2 play important roles cochlear adaptation to elevated sound levels. Three mutations causing inherited progressive hearing loss have been identified. These localize the transmembrane domain 1 (V60L), 2 (G353R) a β-sheet linking ATP binding site pore (D273Y). Herein, were studied human as well P2X2/3 receptors. We measured their of fluorescently labeled derivative (fATP) characterized constructs using patch-clamp technique. The conclusions from our results follows: 1. V60L G353R show robust localization on plasma membrane fATP, whereas mutant D273Y has no fATP. 2. mutation an increased affinity fATP compared with wildtype. 3. expression hP2X2 reduces cell viability, may support its role pathogenesis loss. 4. All distinct phenotypes.

Language: Английский

Citations

0