Markerless mutagenesis enables isoleucine biosynthesis solely from threonine in Methanothermobacter marburgensis DOI Creative Commons

M. Klein,

Angus S. Hilts, Ross T. Fennessy

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT The archaeal model microorganism Methanothermobacter marburgensis has been studied for methane production decades. However, genetic modifications are required to harness M. the generation of novel cell factories industrial-scale commodity and high-value chemicals. Only development tools engineering opens up this possibility. Here, we present establishment first markerless mutagenesis system modification . This allows recycling positive selection markers enables multiple sequential gene deletions or integrations. As a demonstration, clarified postulated isoleucine biosynthesis pathway directly from pyruvate via citramalate synthase (CimA). In doing so, identified putative CimA in deleted coding gene, resulting auxotrophy isoleucine. complementation cimA initiated through constitutive expression led prototrophic growth similar wild type, demonstrating that is essential pyruvate-derived it shown vice versa Escherichia coli before, were able complement with integration synthetic threonine time methanogenic archaeon. was achieved genome characterized thermostable deaminase Geobacillus stearothermophilus successful an alternative paves road future application multi-gene biosynthetic pathways overproduce industrially relevant IMPORTANCE autotrophic, hydrogenotrophic methanogen one best-studied organisms field thermophilic archaea. fact shows robust scalability bioreactor systems makes highly suitable candidate bioprocesses. Additionally, reported study provides enable Scalable cultivation, ability genetically engineer, recent discovery natural amino acid secretion set cornerstone biotechnology economically produce carbon dioxide-derived chemicals at industrial scale.

Language: Английский

Oxygen traces impact on biological methanation from hydrogen and CO2 DOI
Pierre Buffière,

Diana Amaya Ramirez,

Rùben Teixeira Franco

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132080 - 132080

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing the Influence of Pressure and Nutrients on Biological CO2 Methanation Using Response Surface Methodology and Artificial Neural Network—Genetic Algorithm Approaches DOI Creative Commons
Alexandros Chatzis, Konstantinos N. Kontogiannopoulos,

Nikolaos Dimitrakakis

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(1), P. 43 - 43

Published: Jan. 18, 2025

The biological methanation process has emerged as a promising alternative to thermo-catalytic methods due its ability operate under milder conditions. However, challenges such low hydrogen solubility and the need for precise trace element supplementation (Fe(II), Ni(II), Co(II)) constrain methane production yield. This study investigates combined effects of concentrations applied pressure on methanation, addressing their synergistic interactions. Using face-centered composite design, batch mode experiments were conducted optimize production. Response Surface Methodology (RSM) Artificial Neural Network (ANN)—Genetic Algorithm (GA) approaches employed model process. RSM identified optimal ranges elements pressure, while ANN-GA demonstrated superior predictive accuracy, capturing nonlinear relationships with high R² (>0.99) minimal prediction errors. optimization indicated 97.9% efficiency reduced conversion time 15.9 h conditions 1.5 bar metal 25.0 mg/L Fe(II), 0.20 0.02 Co(II). Validation confirmed these predictions deviations below 5%, underscoring robustness models. results highlight metals in enhancing gas–liquid mass transfer enzymatic pathways, demonstrating potential computational modeling experimental validation systems, contributing sustainable

Language: Английский

Citations

0
Analysis and Modelling of the Biological Methanation Process for Power to Gas Applications DOI

Valentina Segneri,

Leonardo Colelli,

Nicola Verdone

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biomethane valorization of POME biogas to fuel grade standards using pressure swing adsorption mediated by alumina formate under non-isothermal conditions DOI

Bahiya Abdullah Jabbar,

Ammar Ali, Thach N. Tu

et al.

Emergent Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Citations

0
Microbial adaptation to H2 improves the conversion of volatile fatty acids to methane during in situ biomethanation even in CO2-depleted conditions DOI

Lucia Braga-Nan,

Éric Trably,

Gaëlle Santa‐Catalina

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132494 - 132494

Published: April 1, 2025

Language: Английский

Citations

0
Markerless mutagenesis enables isoleucine biosynthesis solely from threonine in Methanothermobacter marburgensis DOI Creative Commons

M. Klein,

Angus S. Hilts, Ross T. Fennessy

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT The archaeal model microorganism Methanothermobacter marburgensis has been studied for methane production decades. However, genetic modifications are required to harness M. the generation of novel cell factories industrial-scale commodity and high-value chemicals. Only development tools engineering opens up this possibility. Here, we present establishment first markerless mutagenesis system modification . This allows recycling positive selection markers enables multiple sequential gene deletions or integrations. As a demonstration, clarified postulated isoleucine biosynthesis pathway directly from pyruvate via citramalate synthase (CimA). In doing so, identified putative CimA in deleted coding gene, resulting auxotrophy isoleucine. complementation cimA initiated through constitutive expression led prototrophic growth similar wild type, demonstrating that is essential pyruvate-derived it shown vice versa Escherichia coli before, were able complement with integration synthetic threonine time methanogenic archaeon. was achieved genome characterized thermostable deaminase Geobacillus stearothermophilus successful an alternative paves road future application multi-gene biosynthetic pathways overproduce industrially relevant IMPORTANCE autotrophic, hydrogenotrophic methanogen one best-studied organisms field thermophilic archaea. fact shows robust scalability bioreactor systems makes highly suitable candidate bioprocesses. Additionally, reported study provides enable Scalable cultivation, ability genetically engineer, recent discovery natural amino acid secretion set cornerstone biotechnology economically produce carbon dioxide-derived chemicals at industrial scale.

Language: Английский

Citations

0