Harnessing Microbial Signal Transduction Systems in Natural and Synthetic Consortia for Biotechnological Applications DOI Open Access
Adnan Zahir,

Peter Anyigor Okorie,

Veronica N. Nwobasi

et al.

Biotechnology and Applied Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

ABSTRACT Signal transduction is crucial for communication and cellular response in microbial communities. Consortia rely on it effective communication, responding to changing environmental conditions, establishing community structures, performing collective behaviors. Microbial signal can be through quorum sensing (QS), two‐component systems, biofilm formation, nutrient sensing, chemotaxis, horizontal gene transfer stress response, so forth. The consortium uses small signaling molecules QS regulate expression coordinate intercellular Biofilm formation allows cells adhere aggregate, promoting species interactions resistance. Chemotaxis enables directional movement toward or away from chemical gradients, efficient resource utilization organization within the consortium. In recent years, synthetic consortia have gained attention their potential applications biotechnology bioremediation. Understanding natural important gaining insights into dynamics, evolution, ecological function. It provide strategies biotechnological innovation enhancing biosensors, biodegradation, bioenergy efficiency, waste reduction. This review provides compelling insight that will advance our understanding of dynamics its role orchestrating interactions, which facilitate coordination, cooperation, expression, allocation, trigger specific responses determine success.

Language: Английский

Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms DOI Creative Commons
Jiwei Mao, Hongyu Zhang, Yu Chen

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 74, P. 108401 - 108401

Published: June 27, 2024

Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on distribution cellular resources. The rewiring microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed adverse physiological effects, such as impaired cell growth low product yields. Alleviating imposed undesirable changes has become an increasingly attractive approach constructing robust factories. In this review, we provide brief overview engineering, focusing specifically recent developments strategies diminishing while improving robustness yield. A variety examples are presented showcase promise engineering in facilitating design construction Finally, challenges limitations encountered discussed.

Language: Английский

Citations

24
Bioengineering in Solid-State Fermentation for next sustainable food bioprocessing DOI Creative Commons
Muyideen Olaitan Bamidele, Micheal Bola Bamikale, Eliseo Cárdenas-Hernández

et al.

Next Sustainability, Journal Year: 2025, Volume and Issue: 6, P. 100105 - 100105

Published: Jan. 1, 2025

Language: Английский

Citations

6
Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast DOI Creative Commons
Yuanwei Gou, Dongfang Li, Minghui Zhao

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 19, 2024

Abstract While sanguinarine has gained recognition for antimicrobial and antineoplastic activities, its complex conjugated structure low abundance in plants impede broad applications. Here, we demonstrate the complete biosynthesis of halogenated derivatives using highly engineered yeast strains. To overcome cytotoxicity, establish a splicing intein-mediated temperature-responsive gene expression system (SIMTeGES), simple strategy that decouples cell growth from product synthesis without sacrificing protein activity. debottleneck biosynthesis, identify two reticuline oxidases facilitated functional flavoproteins cytochrome P450 enzymes via molecular engineering. After comprehensive metabolic engineering, report production at titer 448.64 mg L −1 . Additionally, our strain enables fluorinated sanguinarine, showcasing biotransformation through more than 15 biocatalytic steps. This work serves as blueprint utilizing scalable platform biomanufacturing diverse benzylisoquinoline alkaloids derivatives.

Language: Английский

Citations

9
Metabolic Engineering for Squalene Production: Advances and Perspectives DOI
Liang Chai, Jing Che,

Qingsheng Qi

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Squalene is a linear polyunsaturated triterpene which has multiple physiological functions including anticancer, antioxidant, and skin-care. It been widely used in the food, medicine, cosmetics sectors also serves as precursor of triterpenes steroids. Recently, production squalene by microbial cell hosts drawn much attention due to its sustainability, environmental friendliness, great efficiency. In this review, we first introduce recent developments employing factories, especially yeasts. Next, underscore primary metabolic strategies, biosynthetic pathway engineering, manipulation, cofactor organelle engineering. addition traditional engineering discuss some prospective regulation approaches, lipid synthesis, identifying manipulating related transcription factors, dynamic pathway, secretion membrane-impermeable terpenoids. These approaches provide insights for further

Language: Английский

Citations

5
Use of Nicotinamide Mononucleotide as Non-Natural Cofactor DOI Open Access
Tahseena Naaz, Beom Soo Kim

Catalysts, Journal Year: 2025, Volume and Issue: 15(1), P. 37 - 37

Published: Jan. 3, 2025

Nicotinamide mononucleotide (NMN) has emerged as a promising non-natural cofactor with significant potential to transform biocatalysis, synthetic biology, and therapeutic applications. By modulating NAD⁺ metabolism, NMN offers unique advantages in enzymatic reactions, metabolic engineering, regenerative medicine. This review provides comprehensive analysis of NMN’s biochemical properties, mechanisms action, diverse Emphasis is placed on its role addressing challenges multi-enzyme cascades, biofuel production, the synthesis high-value chemicals. The paper also highlights critical research gaps, including need for scalable methods, improved integration into systems, toxicity studies use. Emerging technologies such AI-driven enzyme design CRISPR-based genome engineering are discussed transformative tools optimizing NMN-dependent pathways. Furthermore, synergistic biology innovations, cell-free systems dynamic regulatory networks, explored, paving way precise modular biotechnological solutions. Looking forward, versatility positions it pivotal tool advancing sustainable bioprocessing precision Addressing current limitations through interdisciplinary approaches will enable redefine boundaries innovation. serves roadmap leveraging across scientific industrial domains.

Language: Английский

Citations

0
Artificial intelligence and microbial cellular intelligence for bioprocess and biofuel DOI
Tawaf Ali Shah, Zhihe Li,

Zhiyu Li

et al.

Methods in microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Citations

0
Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts DOI Creative Commons

Wenhui Niu,

Jingxian Zhang,

Lingbo Qu

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 29, 2025

Isoflavones such as daidzein and genistein are naturally occurring compounds found in plants legumes. They have diverse pharmacological activities, making them valuable the food, pharmaceutical, cosmetic industries. Currently, isoflavones mainly obtained through extraction of plant biomass. Chemical synthesis is challenging for most due to complexity their structures. The limited supply cannot meet market demands. Advances synthetic biology provided a sustainable efficient solution production isoflavones, with yeasts often serving microbial chassis biosynthesis. This review summarizes properties specific biosynthetic pathways, technical strategies used engineered isoflavone production. In addition, development state-of-the-art biotechnological environmentally friendly bioactive discussed.

Language: Английский

Citations

0
Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway DOI Creative Commons

Yali Qiu,

Wei Liu, Meiling Wu

et al.

Synthetic and Systems Biotechnology, Journal Year: 2024, Volume and Issue: 9(2), P. 269 - 276

Published: Feb. 28, 2024

Full conversion of glucose and xylose from lignocellulosic hydrolysates is required for obtaining a high ethanol yield. However, share flux in the pentose phosphate pathway (PPP) glycolysis (EMP), with having competitive advantage shared metabolic pathways. In this work, we knocked down ZWF1 to preclude entering PPP. This reduced [NADPH] level disturbed growth on both or xylose, confirming that oxidative PPP, which begins Zwf1p ultimately leads CO2 production, primary source NADPH xylose. Upon depletion, gluconeogenesis necessary generate glucose-6-phosphate, substrate Zwf1p. We re-established regeneration by replacing endogenous NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene TDH3 heterogenous NADP + -GAPDH genes GDH, gapB, GDP1. Among resulting strains, strain BZP1 (zwf1Δ, tdh3:GDP1) exhibited similar consumption rate before but 1.6-fold increased following depletion compared original BSGX001, yield total consumed sugars was 13.5% higher than BSGX001. suggested using EMP instead PPP reduces wasteful cycle excess release Furthermore, used copper-repressing promoter modulate expression optimize timing turning off ZWF1, therefore, determine equilibrium between glucose-xylose co-metabolism. strategy allowed fast early stage fermentation low waste stages fermentation.

Language: Английский

Citations

3
Combined metabolic engineering and lipid droplets degradation to increase vitamin A production in Saccharomyces cerevisiae DOI Creative Commons

J.-B. Lin,

Xiao Bu,

Yibin Lan

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 25, 2024

In microbial cell factories, substrate accessibility to enzyme is a key factor affecting the biosynthesis of natural products. As robust chassis cells for biofuels and bioproducts, Saccharomyces cerevisiae also encounters challenge since different enzymes precursors are typically compartmentalized in organelles. Such spatial separation could largely limit efficiency enzymatic reactions. this study, production hydrophobic product (vitamin A) was highly improved by metabolic engineering combined with degrading lipid droplets (the primary organelle storing β-carotene) achieve efficient contact between β-carotene 15, 15'-β-carotene monooxygenases cerevisiae. To efficiently produce vitamin A cerevisiae, ten (BCMOs) were firstly evaluated. The strain carrying marine bacterium 66A03 (Mb. BCMO) achieved highest titer. Co-adding 10% dodecane 1% dibutylhydroxytoluene increased titer 19.03 mg/L two-phase fermentation. Since most stored LDs while BCMO located cytosol, we developed strategy release from better BCMO. By overexpressing TGL3 TGL4 using an ion-responsive promoter after high accumulation LDs, sequentially degraded, which dramatically production. Finally, tHMG1, ERG20, CrtI introducing Vitreoscilla hemoglobin, reached 219.27 mg/L, 10.52-folds increase over original shake flasks, finally 1100.83 fed-batch effectiveness degradation on promoting formation cleaved has been verified β-ionone synthesis 44.07% yield. Overall, our results highlighted significance sequential overproduction recombinant yeast, that combining improve This integrated can be applied other compounds similar characteristics.

Language: Английский

Citations

2
Building Synthetic Yeast Factories to Produce Fat-soluble Antioxidants DOI
Yuxin Zhao, Zhanyi Yao, V. S. Desai

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103129 - 103129

Published: May 3, 2024

Language: Английский

Citations

1