Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle DOI Creative Commons
Sefli Sri Wahyu Effendi, I‐Son Ng

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 2038 - 2044

Published: July 2, 2024

Carbon dioxide emission and acidification during chemical biosynthesis are critical challenges toward microbial cell factories' sustainability efficiency. Due to its acidophilic traits among workhorse lineages, the probiotic Escherichia coli Nissle (EcN) has emerged as a promising bioproducer. However, EcN lacks CO2-fixing system. Herein, was equipped with simultaneous CO2 fixation system subsequently utilized produce low-emission 5-aminolevulinic acid (5-ALA). Two different artificial CO2-assimilating pathways were reconstructed: novel ribose-1,5-bisphosphate (R15P) route conventional ribulose-5-phosphate (Ru5P) route. CRISPRi employed target pfkAB zwf genes in order redirect carbon flux. As expected, design successfully strengthened fixation. The via R15P resulted high biomass, while engineered Ru5P acquired highest 5-ALA suppressed release by 77%. production synchronized through fine-tuning non-native CRISPRi.

Language: Английский

Harnessing the CRISPR/Cas9 in filamentous fungi for the production of secondary metabolites DOI

Md Dilshad Karim,

Md. Abuhena,

Lutfur Rahman

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

0
Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle DOI Creative Commons
Sefli Sri Wahyu Effendi, I‐Son Ng

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 2038 - 2044

Published: July 2, 2024

Carbon dioxide emission and acidification during chemical biosynthesis are critical challenges toward microbial cell factories' sustainability efficiency. Due to its acidophilic traits among workhorse lineages, the probiotic Escherichia coli Nissle (EcN) has emerged as a promising bioproducer. However, EcN lacks CO2-fixing system. Herein, was equipped with simultaneous CO2 fixation system subsequently utilized produce low-emission 5-aminolevulinic acid (5-ALA). Two different artificial CO2-assimilating pathways were reconstructed: novel ribose-1,5-bisphosphate (R15P) route conventional ribulose-5-phosphate (Ru5P) route. CRISPRi employed target pfkAB zwf genes in order redirect carbon flux. As expected, design successfully strengthened fixation. The via R15P resulted high biomass, while engineered Ru5P acquired highest 5-ALA suppressed release by 77%. production synchronized through fine-tuning non-native CRISPRi.

Language: Английский

Citations

1