In vitro development of a muscle-tendon junction construct using decellularised extracellular matrix: Effect of cyclic tensile loading DOI Creative Commons
Nodoka Iwasaki, Marta Roldo, Aikaterina Karali

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 161, P. 213873 - 213873

Published: April 28, 2024

The muscle tendon junction (MTJ) plays a crucial role in transmitting the force generated by muscles to and then bone. Injuries such as tears strains frequently happen at MTJ, where regenerative process is limited due poor vascularization complex structure of tissue. Current solutions for complete tear MTJ have not been successful therefore, development tissue-engineered may provide more effective treatment. In this study, decellularised extracellular matrix (DECM) derived from sheep was used scaffold order with relevant mechanical properties differentiation cues growth factors. Human mesenchymal stem cells (MSCs) were seeded on DECM 10 % cyclic strain applied using bioreactor. MSCs cultured showed significantly higher gene protein expression markers collagen 22, paxillin talin, than 2D culture. Although 22 without strain, reduced other observed when applied. combined enhanced myogenic differentiation, while tenogenic compared static cultures DECM. For first time, these results that can induce marker MSCs, however, effect culture needs further investigation.

Language: Английский

In vitro development of a muscle-tendon junction construct using decellularised extracellular matrix: Effect of cyclic tensile loading DOI Creative Commons
Nodoka Iwasaki, Marta Roldo, Aikaterina Karali

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 161, P. 213873 - 213873

Published: April 28, 2024

The muscle tendon junction (MTJ) plays a crucial role in transmitting the force generated by muscles to and then bone. Injuries such as tears strains frequently happen at MTJ, where regenerative process is limited due poor vascularization complex structure of tissue. Current solutions for complete tear MTJ have not been successful therefore, development tissue-engineered may provide more effective treatment. In this study, decellularised extracellular matrix (DECM) derived from sheep was used scaffold order with relevant mechanical properties differentiation cues growth factors. Human mesenchymal stem cells (MSCs) were seeded on DECM 10 % cyclic strain applied using bioreactor. MSCs cultured showed significantly higher gene protein expression markers collagen 22, paxillin talin, than 2D culture. Although 22 without strain, reduced other observed when applied. combined enhanced myogenic differentiation, while tenogenic compared static cultures DECM. For first time, these results that can induce marker MSCs, however, effect culture needs further investigation.

Language: Английский

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