Environmental DNA (eDNA): an eco-friendly approach for conservation of Indian freshwater diversity—a review DOI
Ashish Sahu, Mahender Singh, Rishikesh Krishan Laxmi

et al.

Environmental Science and Pollution Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский

Exploitation of environmental DNA (eDNA) for ecotoxicological research: A critical review on eDNA metabarcoding in assessing marine pollution DOI
Sakib Tahmid Rishan, Richard J. Kline, Md Saydur Rahman

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 351, P. 141238 - 141238

Published: Jan. 21, 2024

Language: Английский

Citations

8

An overlooked source of false positives in eDNA-based biodiversity assessment and management DOI
Wei Xiong, Hugh J. MacIsaac, Aibin Zhan

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 358, P. 120949 - 120949

Published: April 23, 2024

Language: Английский

Citations

6

Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems DOI

Suxiang Lu,

Honghui Zeng, Fan Xiong

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(7), P. 1368 - 1384

Published: March 18, 2024

Language: Английский

Citations

4

Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems DOI Creative Commons
Gert‐Jan Jeunen,

Sadie Mills,

Stefano Mariani

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174354 - 174354

Published: June 30, 2024

Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing time-consuming process water filtration. Designing a novel passive sampler that does not require extensive sample handling time and can be connected to ocean-going vessels without impeding normal underway activities has potential rapidly upscale global efforts onboard world's oceanic fleet. Here, we demonstrate utility an artificial sponge continuous pump seawater system as means enable biomonitoring. We compared performance this sampling protocol with standard filtration at six locations during research voyage from New Zealand Antarctica early 2023. Eukaryote metabarcoding mitochondrial COI gene revealed no significant difference phylogenetic α-diversity between methods both delineated progressive reduction number Zero-Radius Operational Taxonomic Units (ZOTUs) increased latitudes. While comparable trends geographical community compositions, distinct clusters were identified for each location. Additionally, greater variability replicates was observed samplers, resulting estimated level replication needed recover 90 % biodiversity. Furthermore, traditional failed detect three phyla extrapolation analysis larger ZOTUs all locations. Our results eDNA highlight areas where emerging technology could improved, thereby large-scale offshore marine leveraging fleet interfering activities.

Language: Английский

Citations

4

A new flow path: eDNA connecting hydrology and biology DOI Creative Commons
Dawn URycki, Anish Kirtane, Rachel Aronoff

et al.

Wiley Interdisciplinary Reviews Water, Journal Year: 2024, Volume and Issue: 11(6)

Published: July 29, 2024

Abstract Environmental DNA (eDNA) has revolutionized ecological research, particularly for biodiversity assessment in various environments, most notably aquatic media. analysis allows non‐invasive and rapid species detection across multiple taxonomic groups within a single sample, making it especially useful identifying rare or invasive species. Due to dynamic hydrological processes, eDNA samples from running waters may represent broad contributing areas, which is convenient biomonitoring perspective but also challenging, as knowledge required meaningful biological interpretation. Hydrologists could benefit address unsolved questions, concerning water movement through catchments. While naturally occurring abiotic tracers have advanced our understanding of age distribution catchments, example, current geochemical cannot fully elucidate the timing flow paths landscapes. Conversely, tracers, owing their immense diversity interactions with environment, offer more detailed information on sources stream. The informational capacity tracer, however, determined by ability interpret complex heterogeneity at study site, arguably requires both expertise. As data become increasingly available part campaigns, we argue that accompanying surveys observations enhance processes; identify opportunities, challenges, needs further interdisciplinary collaboration; highlight eDNA's potential bridge between hydrology biology, foster domains. This article categorized under: Science Water > Hydrological Processes Methods Life Nature Freshwater Ecosystems

Language: Английский

Citations

4

The One Health Approach in Urban Ecosystem Rehabilitation: An Evidence-Based Framework for Designing Sustainable Cities DOI Creative Commons
Antonia Bruno, Irene Arnoldi, Benedetta Barzaghi

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(10), P. 110959 - 110959

Published: Sept. 14, 2024

Rapid urbanization has led to negative, and sometimes unintended, consequences on biodiversity human health. While cities offer numerous advantages in meeting the basic needs of a growing population, they also pose less apparent longer-term health costs. To address multifaceted impacts urbanization, an evidence-based design framework for establishing mitigation regeneration actions is essential. Via "One Health" approach, this perspective provides recommendations strategies urban ecosystem rehabilitation future cities, placing services at core designing healthy sustainable spaces. The we propose based Hub Spoke model integrate diverse perspectives from public private sectors declined six-building-blocks structure. This will ensure that efforts are sustainable, health-centered, socially inclusive, grounded high-quality data, reinforcing essential connection between environments thriving communities.

Language: Английский

Citations

4

Monitoring terrestrial rewilding with environmental DNA metabarcoding: a systematic review of current trends and recommendations DOI Creative Commons

Clare Cowgill,

James Gilbert, Ian Convery

et al.

Frontiers in Conservation Science, Journal Year: 2025, Volume and Issue: 5

Published: Jan. 2, 2025

Introduction Rewilding, the facilitation of self-sustaining and resilient ecosystems by restoring natural processes, is an increasingly popular conservation approach potential solution to biodiversity climate crises. Outcomes rewilding can be unpredictable, monitoring essential determine whether are recovering. Metabarcoding, particularly environmental DNA (eDNA), revolutionizing could play important role in understanding impacts but has mostly been applied within aquatic systems. Methods This systematic review focuses on applications eDNA metabarcoding terrestrial monitoring, with additional insights from bulk ingested DNA. We examine publication trends, choice sampling substrate focal taxa, investigate how well performs compared other methods (e.g. camera trapping). Results Terrestrial represented a small proportion total papers, forests most studied system, soil water substrates, vertebrates targeted taxa. Most studies focused measuring species richness, few included analyzes functional diversity. Greater richness was found when using multiple took this approach. Metabarcoding did not consistently outperform terms number vertebrate taxa detected, likely influenced marker, habitat. Discussion Our findings indicate that metabarcoding, eDNA, key rewilding, further ground- truthing needed establish appropriate experimental pipelines for target system interest. Systematic Review Registration https://osf.io/38w9q/?view_only=47fdab224a7a43d298eccbe578f1fcf0 , identifier 38w9q.

Language: Английский

Citations

0

Environmental DNA and its role in identifying yeast biodiversity in mangrove ecosystem: A literature review DOI Creative Commons

Lila Kusuma Rahayu,

Tri Retnaningsih Soeprobowati, Budi Warsito

et al.

E3S Web of Conferences, Journal Year: 2025, Volume and Issue: 605, P. 03005 - 03005

Published: Jan. 1, 2025

Yeast biodiversity in mangrove ecosystems is crucial for their health and ecological functions. However, traditional methods often fail to detect yeast species that are difficult culture or slow growing, leaving a significant portion of this undetected. This study explores the use environmental DNA (eDNA) as an alternative method identifying ecosystems, addressing data gaps left by conventional methods. Data collected from various relevant literature sources, including scientific journals, books, research reports. The review reveals eDNA analysis can improve conservation management providing robust assessments. Future should focus on understanding persistence, implications, improving bioinformatics better interpretation. In-depth long-term studies recommended temporal spatial dynamics communities ecosystems. Further validation conditions needed ensure reliability. Research interactions with other ecosystem components impact human activities like pollution habitat change also important.

Language: Английский

Citations

0

International aquatic invasive species early detection efforts in the St. Clair-Detroit River System: A decadal review DOI Creative Commons
Kristen Towne,

Matthew Cowley,

Mark Jonathan D'Aguiar

et al.

Journal of Great Lakes Research, Journal Year: 2025, Volume and Issue: unknown, P. 102531 - 102531

Published: Feb. 1, 2025

Language: Английский

Citations

0

Optimizing eDNA Metabarcoding Techniques for Assessing Arthropod Communities in Tree‐Related Microhabitats DOI Creative Commons
Mohammad Jamil Shuvo, Tasmina Tabassum, Gernot Segelbacher

et al.

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

ABSTRACT Understanding the diversity and ecological roles of arthropods within tree‐related microhabitats (TreMs) is crucial for forest ecosystem conservation management. In our study, we aimed to identify most effective environmental DNA (eDNA) metabarcoding approach capturing ecologically important arthropod species primarily inhabiting near‐ground‐level TreMs. We evaluated use COI 16S primers eDNA compared direct indirect sampling methods, including lying deadwood sediment (LDS), standing (SDS), soil (SS), tree surface roller (TSRS). Our results indicated significant biases challenges, particularly in primer selection, with outperforming taxonomic resolution taxa. TSRS method effectively captured 408 OTUs at level, highest number associated TreMs other approaches. Direct from sediments revealed a higher abundance fungi than arthropods, impacting estimates. also observed habitat‐specific preferences among certain methods distinct findings underscore importance carefully selecting validating studies provide insights into complexity communities Optimized will advance monitoring techniques ecosystems inform efforts preserve

Language: Английский

Citations

0