Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Feb. 25, 2020

Impacts of climate change like global warming, drought, flooding, and other extreme events are posing severe challenges to crop production. Contribution Brassica napus towards the oilseed industry makes it an essential component international trade agroeconomics. Consequences from increasing occurrences multiple abiotic stresses on this leading agroeconomic losses making vital endow B. with ability survive maintain yield when faced simultaneous exposure stresses. For improved understanding stress sensing machinery, there is a need for analyzing regulatory pathways stress-responsive genes elements such as non-coding RNAs. However, our these their interactions in far complete. This review outlines current knowledge role imparting tolerance napus. Analysis network crosstalk through omics data mining now possible unravel underlying complexity required signalling plants. Novel biotechnological approaches transgene-free genome editing utilization nanoparticles gene delivery tools also discussed. These can contribute providing solutions developing resilient varieties reduced limitations. The potential synthetic biology engineer modify networks fine-tuning plant responses adaption highlighted.

Language: Английский

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Current perspectives of lncRNAs in abiotic and biotic stress tolerance in plants

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Abiotic/biotic stresses pose a major threat to agriculture and food security by impacting plant growth, productivity quality. The discovery of extensive transcription large RNA transcripts that do not code for proteins, termed long non-coding RNAs (lncRNAs) with sizes larger than 200 nucleotides in length, provides an important new perspective on the centrality gene regulation. In plants, lncRNAs are widespread fulfill multiple biological functions stress response. this paper, research advances function lncRNA response were summarized, like as Natural Antisense Transcripts (NATs), Competing Endogenous (ceRNAs) Chromatin Modification etc. And act key regulatory hub several phytohormone pathways, integrating abscisic acid (ABA), jasmonate (JA), salicylic (SA) redox signaling many abiotic/biotic stresses. Moreover, conserved sequence motifs structural enriched within stress-responsive may also be responsible lncRNAs, it will provide focus strategy research. Taken together, we highlight unique role adverse environmental conditions different aspects growth development. We envisage improved understanding mechanisms which regulate further promote development unconventional approaches breeding stress-resistant crops.

Language: Английский

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Genome-wide investigation of regulatory roles of lncRNAs in response to heat and drought stress in Brassica juncea (Indian mustard)

Environmental and Experimental Botany, Journal Year: 2019, Volume and Issue: 171, P. 103922 - 103922

Published: Nov. 5, 2019

Language: Английский

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MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops

Life, Journal Year: 2021, Volume and Issue: 11(4), P. 289 - 289

Published: March 28, 2021

Crop yield is challenged every year worldwide by changing climatic conditions. The forecasted scenario urgently demands stress-tolerant crop varieties to feed the ever-increasing global population. Molecular breeding and genetic engineering approaches have been frequently exploited for developing crops with desired agronomic traits. Recently, microRNAs (miRNAs) emerged as powerful molecules, which potentially serve expression markers during stress miRNAs are small non-coding endogenous RNAs, usually 20–24 nucleotides long, mediate post-transcriptional gene silencing fine-tune regulation of many abiotic- biotic-stress responsive genes in plants. function specifically pairing target mRNAs, inducing their cleavage or repressing translation. This review focuses on exploration functional role regulating plant responses abiotic biotic stresses. Moreover, a methodology also discussed mine stress-responsive from enormous amount transcriptome data available public domain generated using next-generation sequencing (NGS). Considering mediating responses, these molecules may be explored novel targets varieties.

Language: Английский

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Long non-coding RNA transcriptome landscape of anthers at different developmental stages in response to drought stress in tomato

Genomics, Journal Year: 2022, Volume and Issue: 114(4), P. 110383 - 110383

Published: May 10, 2022

Pollen development is particularly susceptible to drought stress. Long non-coding RNAs (lncRNAs) are known play a role in plant and responses However, the expression profile putative function of lncRNAs drought-induced male sterility remain largely unknown. In this study, we investigated lncRNA transcriptome landscape tomato anthers at early late stages-tetrad-vacuolated microspore (TED-VUM) binucleate-mature pollen (BIN-MP) anthers, respectively-in response stress using RNA-sequencing. total, identified 67,770 lncRNAs, which 3053 were responsive. Interestingly, there more differentially expressed (DE) TED-VUM (2879) than BIN-MP (174) was consistent with being sensitive. Functional enrichment analysis revealed that target genes DE significantly enriched diverse metabolic processes, including carbohydrate metabolism hormone synthesis. Co-expression also 1407 strongly co-expressed 8 involved (abscisic acid jasmonic acid) (sucrose starch) metabolisms tapetum development, highlighting potential lncRNA-target-gene modulation anther under Our results serve as baseline for future investigations reproductive

Language: Английский

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Genome-wide analysis of long non-coding RNAs in sugar beet (Beta vulgaris L.) under drought stress

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 14, 2023

Drought stress is one of the most severe abiotic stresses that restrict global crop production. Long non-coding RNAs (lncRNAs) have been proved to play a key role in response drought stress. However, genome-wide identification and characterization drought-responsive lncRNAs sugar beet still lacking. Thus, present study focused on analyzing under We identified 32017 reliable by strand-specific high-throughput sequencing. A total 386 differentially expressed (DElncRNAs) were found The significantly upregulated downregulated TCONS_00055787 (upregulated more than 6000 fold) TCONS_00038334 (downregulated 18000 fold), respectively. Quantitative real-time PCR results exhibited high concordance with RNA sequencing data, which conformed expression patterns based highly reliable. In addition, we predicted 2353 9041 transcripts estimated be cis- trans-target genes lncRNAs. As revealed Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analysis, target DElncRNAs enriched organelle subcompartment, thylakoid, endopeptidase activity, catalytic developmental process, lipid metabolic polymerase transferase flavonoid biosynthesis several other terms associated tolerance. Moreover, 42 as potential miRNA mimics. LncRNAs important effects plant adaptation conditions through interaction protein-encoding genes. leads greater insights into lncRNA biology offers candidate regulators for improving tolerance cultivars at genetic level.

Language: Английский

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Melatonin enhances cadmium tolerance in rice via long non-coding RNA-mediated modulation of cell wall and photosynthesis

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133251 - 133251

Published: Dec. 14, 2023

Language: Английский

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Genome-Wide Identification and Characterization of Drought Stress Responsive microRNAs in Tibetan Wild Barley

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2795 - 2795

Published: April 17, 2020

Drought stress is a major obstacle to agricultural production. Tibetan wild barley with rich genetic diversity useful for drought-tolerant improvement of cereals. MicroRNAs (miRNAs) play critical roles in controlling gene expression response various environment perturbations plants. However, the genome-wide profiles miRNAs and their targets drought are largely unknown barley. In this study, polyethylene glycol (PEG) induced hydroponic experiment was performed, from roots two contrasting genotypes XZ5 (drought-tolerant) XZ54 (drought-sensitive), one cultivated Tadmor generated by high-throughput sequencing were compared. There 69 conserved 1574 novel dataset three under control conditions. Among them, seven 36 showed significantly genotype-specific patterns stress. And 12 further regarded as tolerant associated XZ5, which mostly participate expression, metabolism, signaling transportation, suggesting that they target genes important plant tolerance. This first comparation study on miRNA transcriptome differing tolerance cultivar PEG treatment. Further results revealed candidate regulation mechanism Our findings provide valuable understandings functional characterization

Language: Английский

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A deep learning model for plant lncRNA-protein interaction prediction with graph attention

Molecular Genetics and Genomics, Journal Year: 2020, Volume and Issue: 295(5), P. 1091 - 1102

Published: May 15, 2020

Language: Английский

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Genome-wide characterization of drought-responsive long non-coding RNAs in sorghum (Sorghum bicolor)

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 214, P. 108908 - 108908

Published: July 4, 2024

Language: Английский

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