Identification of methyltransferase and demethylase genes and their expression profiling under biotic and abiotic stress in pigeon pea (Cajanus cajan [L.] Millspaugh)

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 16, 2025

The methylation- demethylation dynamics of RNA plays major roles in different biological functions, including stress responses, plants. m6A methylation is orchestrated by a coordinated function methyl transferases (writers) and demethylases (Erasers). Genome-wide analysis genes involved was performed pigeon pea. Blast search, using Arabidopsis gene sequences, resulted the identification two (CcMTA70, CcMTB70), encoding adaptor proteins for (CcFIPA CcFIPB) 10 demethylase (ALKBH) (CcALKBH1A, CcALKBH1B, CcALKBH1C, CcALKBH2, CcALKBH8, CcALKBH8A, CcALKBH8B, CcALKBH9, CcALKBH10A CcALKBH10B) pea genome. identified were analyzed through phylogenetic relationship, chromosomal position, structure, conserved motif, domain subcellular location prediction etc. These structural analyses categorization MTs FIPs into one group, i.e., CcMTA/B CcFIPA/B, respectively; ALKBHs four groups, viz. CcALKBH1/2, CcALKBH9 CcALKBH10. Relative expression various tissues at developmental stages revealed highest level leaf least root. CcMTs CcFIPs had similar patterns expression, CcALKBH10B demonstrated CcALKBH2 lowest all analyzed. CcALKBH8 showed induction upon exposure to heat stress, during drought, salt biotic (Helicoverpa armigera infestation) stresses. present study would pave way detailed molecular characterization its involvement regulation.

Language: Английский

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Functional identification of JrMYBs gene in Walnut (Juglans regia) demonstrates it enhances drought stress tolerance

Physiology and Molecular Biology of Plants, Journal Year: 2025, Volume and Issue: 31(3), P. 375 - 387

Published: March 1, 2025

Language: Английский

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Genome-wide identification and characterization of ALKB homolog gene family in wheat (Triticum aestivum L.)

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

N6-methyladenosine (m6A) is the most prevalent posttranscriptional modification in eukaryotic mRNAs. AlkB homologs (ALKBHs) are involved plant responses to stress by modulating m6A methylation. However, homologous genes wheat remain largely uncharacterized. In this study, 30 ALKBH were identified wheat, and analyzed their physicochemical properties. The phylogenetic analysis allowed classification of these into seven distinct subfamilies. Additionally, conserved domains, motif compositions, gene structures, chromosomal localization, synteny, predicted cis-acting elements within promoters examined. Expression revealed that TaALKBH9B-5 exhibited highest expression its demethylase activity was investigated. Furthermore, significantly upregulated response abscisic acid treatment cold stress, indicating a positive regulatory trend. conclusion, study provides comprehensive genomic assessment TaALKBH family offers theoretical framework for understanding role TaALKBH9B abiotic wheat.

Language: Английский

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Genome-wide analysis of the cotton COBRA-like gene family and functional characterization of GhCOBL22 in relation to drought tolerance

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 23, 2024

Abstract Background The COBRA-like (COBL) gene family is a crucial glycosylphosphatidylinositol (GPI)-anchored proteins that participate in various biological processes plants by regulating the arrangement of cell wall microfibrils. While functions COBL genes have been analyzed several plant species, their roles cotton’s response to abiotic stress remain unexplored. Results This study identified and characterized Gossypium hirsutum , revealing total 39 members classified into five subgroups. Transcriptome analysis indicated transcription levels GhCOBL were upregulated following PEG treatment, with GhCOBL22 being significantly induced. Further silencing through virus-induced (VIGS) technology demonstrated this gene’s reduced drought tolerance. Under conditions, activities superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) enzymes, along proline (PRO) content, lower -silenced compared control plants, while accumulation malondialdehyde (MDA) was higher. Moreover, also led reductions cellulose, hemicellulose, lignin content cotton leaves. Conclusion A systematic survey structure, motif composition, evolutionary relationships conducted . Subsequent expression functional studies plays significant role These findings enhance our understanding highlight critical stress.

Language: Английский

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The Roles of RNA Modifications in Regulating Chloroplast Performance and Photosynthesis Efficiency

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11912 - 11912

Published: Nov. 6, 2024

The regulation of gene expression is crucial for maintaining cellular activities and responding to environmental stimuli. RNA molecules are central this regulatory network, influencing transcription, post-transcriptional processing, translation. Recent advancements have expanded our understanding modifications beyond the nucleus, highlighting their impact on chloroplast function photosynthesis efficiency. Chloroplasts, essential photosynthesis, rely precise genetic adapt changes. modifications, such as methylation pseudouridylation, critical in regulating stability, This review summarizes current knowledge how affect photosynthesis. It discusses roles specific occurring RNA, including N6-methyladenosine (m

Language: Английский

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