Physiological, anatomical, and transcriptomic analyses reveal the potential mechanism of resistance of Akebia trifoliata to acid rain stress and mitigation effects of curcumin DOI Creative Commons

Xingmei Tao,

Kai Wang,

Xiaoxu Bi

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Acid rain is a global ecological issue severely threatening crop growth. Curcumin (CUR), natural antioxidant, can enhance the tolerance of plants to abiotic stresses via physiological and molecular modes. As both medicine food, Akebia trifoliata exhibits high economic value. The resistance mechanism A. acid mitigation effects CUR remain unclear. Therefore, in this study, we investigated plant growth, characteristics leaves, anatomical structure, gene expression under stress before after treatment with exogenous CUR. results indicated that stress, contents chlorophyll a, b, total chlorophyll, starch thicknesses upper lower epidermis leaves decreased by 58.16%, 77.88%, 64.77%, 63.85%, 58.93%, 35.57%, respectively. Moreover, MDA, soluble sugar, protein, proline production rate oxygen free radicals increased 82.55%, 43.20%, 44.55%, 64.40% 345.77%, This suggested affected growth development trifoliata. resisted increasing SOD CAT activities; thickness leaf, palisade tissue, spongy tissue; ratio palisade/spongy tissue. However, could effectively facilitate maintain integrity structure relieve damages caused 50 µmol/L (CUR50) was most optimal concentration. Transcriptomic analysis revealed CUR0 vs Control, CUR50 had 2978, 1760, 323 DEGs, KEGG pathway enrichment these DEGs were involved eight pathways, among which protein processing endoplasmic reticulum, hormone signal transduction, phenylpropanoid biosynthesis, sucrose metabolism key metabolic pathways mitigated stress. study potential response physiological, anatomical, transcriptomic analyses, thereby providing theoretical references for phytoremediation zone.

Language: Английский

Adaptive Responses of Hormones to Nitrogen Deficiency in Citrus sinensis Leaves and Roots DOI Creative Commons

Dan Hua,

Rong-Yu Rao,

Wenshu Chen

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(14), P. 1925 - 1925

Published: July 12, 2024

Some citrus orchards in China often experience nitrogen (N) deficiency. For the first time, targeted metabolomics was used to examine N-deficient effects on hormones sweet orange (Citrus sinensis (L.) Osbeck cv. Xuegan) leaves and roots. The purpose validate hypothesis that play a role N deficiency tolerance by regulating root/shoot dry weight ratio (R/S), root system architecture (RSA), leaf senescence. deficiency-induced decreases gibberellins indole-3-acetic acid (IAA) levels increases cis(+)-12-oxophytodienoic (OPDA) levels, ethylene production, salicylic (SA) biosynthesis might contribute reduced growth accelerated senescence leaves. increased formation be caused 1-aminocyclopropanecarboxylic OPDA decreased abscisic (ABA). R/S, altered RSA, delayed lowering cytokinins, jasmonic acid, OPDA, ABA SA biosynthesis, increasing 5-deoxystrigol maintaining IAA gibberellin homeostasis. unchanged concentration roots involved leaf-to-root transport. different responses of regulation senescence, thus improving use efficiency, remobilization ability acquire N, hence conferring tolerance.

Language: Английский

Citations

4
Physiological, anatomical, and transcriptomic analyses reveal the potential mechanism of resistance of Akebia trifoliata to acid rain stress and mitigation effects of curcumin DOI Creative Commons

Xingmei Tao,

Kai Wang,

Xiaoxu Bi

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Acid rain is a global ecological issue severely threatening crop growth. Curcumin (CUR), natural antioxidant, can enhance the tolerance of plants to abiotic stresses via physiological and molecular modes. As both medicine food, Akebia trifoliata exhibits high economic value. The resistance mechanism A. acid mitigation effects CUR remain unclear. Therefore, in this study, we investigated plant growth, characteristics leaves, anatomical structure, gene expression under stress before after treatment with exogenous CUR. results indicated that stress, contents chlorophyll a, b, total chlorophyll, starch thicknesses upper lower epidermis leaves decreased by 58.16%, 77.88%, 64.77%, 63.85%, 58.93%, 35.57%, respectively. Moreover, MDA, soluble sugar, protein, proline production rate oxygen free radicals increased 82.55%, 43.20%, 44.55%, 64.40% 345.77%, This suggested affected growth development trifoliata. resisted increasing SOD CAT activities; thickness leaf, palisade tissue, spongy tissue; ratio palisade/spongy tissue. However, could effectively facilitate maintain integrity structure relieve damages caused 50 µmol/L (CUR50) was most optimal concentration. Transcriptomic analysis revealed CUR0 vs Control, CUR50 had 2978, 1760, 323 DEGs, KEGG pathway enrichment these DEGs were involved eight pathways, among which protein processing endoplasmic reticulum, hormone signal transduction, phenylpropanoid biosynthesis, sucrose metabolism key metabolic pathways mitigated stress. study potential response physiological, anatomical, transcriptomic analyses, thereby providing theoretical references for phytoremediation zone.

Language: Английский

Citations

0