Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered

Critical Reviews in Environmental Science and Technology, Journal Year: 2019, Volume and Issue: 50(19), P. 2016 - 2059

Published: Dec. 3, 2019

Aquatic environments are identified as an ideal setting for acquisition and dissemination of antibiotic resistance, human exposure to resistant bacteria (ARB) resistance genes (ARGs) in aquatic may pose additional health risk. Quantitative microbial risk assessment (QMRA) has been suggested a suitable method evaluate quantify this However, information about the ARB ARGs is lacking many scenarios dose-response models regarding infections not developed yet. This review summarizes current knowledge highlights challenging questions remaining be answered better forecast risks caused by water environments. The include what missing needed exposing environments? markers ARB/ARGs contamination how frequently do ARG selection propagation occur there any unknown hot spots? Studies on above topics will contribute management its health.Abbreviations3GC3rd generation cephalosporinsARBAntibiotic bacteriaARGAntibiotic geneCFUColony forming unitDBPDisinfection by-productseDNAExtracellular DNAEPSExtracellular polymeric substancesHGTHorizontal gene transferISCRInsertion sequence common regionMARMultiple resistantMICMinimum inhibitory concentrationMGEMobile genetic elementsMSWMunicipal solid wasteQMRAQuantitative assessmentVBNCViable but non-culturableWWTPWastewater treatment plant

Language: Английский

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Both silver ions and silver nanoparticles facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes

Water Research, Journal Year: 2019, Volume and Issue: 169, P. 115229 - 115229

Published: Oct. 25, 2019

Language: Английский

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Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera

Environment International, Journal Year: 2019, Volume and Issue: 129, P. 478 - 487

Published: May 30, 2019

The spread of antibiotic resistance has become a major concern for public health. As emerging contaminants, various metallic nanoparticles (NPs) and ionic heavy metals have been ubiquitously detected in environments. Although previous studies indicated NPs could exhibit co-selection effects resistance, little is known about whether how they promote via horizontal gene transfer across bacterial genera. This study, we report both CuO copper ions (Cu2+) stimulate the conjugative multiple-drug genes. When exposing bacteria to or Cu2+ at environmental-relevant sub-inhibitory concentrations (e.g., 1-100 μmol/L), conjugation frequencies plasmid-encoded genes genera (i.e., from Escherichia coli Pseudomonas putida) were significantly enhanced (p < 0.05). over-production reactive oxygen species played crucial role promoting transfer. Genome-wide RNA protein sequencing suggested expressional levels proteins related oxidative stress, cell membrane permeability, pilus generation up-regulated under exposure study provides insights contributions on resistance.

Language: Английский

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Monitoring antibiotic resistance genes in wastewater treatment: Current strategies and future challenges

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 783, P. 146964 - 146964

Published: April 7, 2021

Language: Английский

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Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation

The ISME Journal, Journal Year: 2020, Volume and Issue: 14(8), P. 2179 - 2196

Published: May 18, 2020

Abstract Antibiotic resistance is a serious global threat for public health. Considering the high abundance of cell-free DNA encoding antibiotic genes (ARGs) in both clinical and environmental settings, natural transformation an important horizontal gene transfer pathway to transmit resistance. It acknowledged that antibiotics are key drivers disseminating resistance, yet contributions non-antibiotic pharmaceuticals on ARGs overlooked. In this study, we report some commonly consumed pharmaceuticals, at clinically environmentally relevant concentrations, significantly facilitated spread through uptake exogenous ARGs. This included nonsteroidal anti-inflammatories, ibuprofen, naproxen, diclofenac, lipid-lowering drug, gemfibrozil, β-blocker propranolol. Based results flow cytometry, whole-genome RNA sequencing proteomic analysis, enhanced was affiliated with promoted bacterial competence, stress levels, over-produced reactive oxygen species increased cell membrane permeability. addition, mathematical model proposed calibrated predict dynamics during exposure pharmaceuticals. Given consumption these findings reveal new concerns regarding dissemination exacerbated by

Language: Английский

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Nonnutritive sweeteners can promote the dissemination of antibiotic resistance through conjugative gene transfer

The ISME Journal, Journal Year: 2021, Volume and Issue: 15(7), P. 2117 - 2130

Published: Feb. 15, 2021

Abstract Antimicrobial resistance (AMR) poses a worldwide threat to human health and biosecurity. The spread of antibiotic genes (ARGs) via conjugative plasmid transfer is major contributor the evolution this resistance. Although permitted as safe food additives, compounds such saccharine, sucralose, aspartame, acesulfame potassium that are commonly used nonnutritive sweeteners have recently been associated with shifts in gut microbiota similar those caused by antibiotics. As antibiotics can promote (ARGs), we hypothesize these could effect. Here, demonstrate for first time plasmid-mediated three established conjugation models between same different phylogenetic strains. real-time dynamic process was visualized at single-cell level. Bacteria exposed tested exhibited increased reactive oxygen species (ROS) production, SOS response, gene transfer. In addition, cell membrane permeability both parental bacteria under exposure compounds. expression involved ROS detoxification, significantly upregulated sweetener treatment. conclusion, enhances bacteria. Our findings provide insight into AMR indicate potential risk presence sweeteners.

Language: Английский

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Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress

The ISME Journal, Journal Year: 2021, Volume and Issue: 15(10), P. 2969 - 2985

Published: May 3, 2021

The bacterial infection that involves antimicrobial resistance is a rising global threat to public health. Chlorine-based water disinfection processes can inactivate antibiotic resistant bacteria. However, at the same time, these may cause release of genes into as free DNA, and consequently increase risk disseminate via natural transformation. Presently, little known about contribution residual chlorine affecting transformation extracellular (ARGs). This study investigates whether chloramine promote ARGs how this occur. We reveal both chlorine, practically relevant concentrations, significantly stimulated plasmid-encoded by recipient Acinetobacter baylyi ADP1, up 10-fold increase. underlying mechanisms underpinning increased transformations were revealed. Disinfectant exposure induced series cell responses, including levels reactive oxygen species (ROS), membrane damage, ROS-mediated DNA stress response. These effects thus culminated in enhanced ARGs. promoted was observed when exposing disinfectant-pretreated A. plasmid. In contrast, after pretreating plasmid with disinfectants, decreased due damage integrity. findings provide important insight on roles disinfectants horizontal transfer ARGs, which could be crucial management our systems.

Language: Английский

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Antibiotic perturbations to the gut microbiome

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 21(12), P. 772 - 788

Published: July 25, 2023

Language: Английский

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Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(9), P. 5479 - 5596

Published: April 28, 2021

Tripartite efflux pumps and the related type 1 secretion systems (T1SSs) in Gram-negative organisms are diverse function, energization, structural organization. They form continuous conduits spanning both inner outer membrane composed of three principal components—the energized transporters (belonging to ABC, RND, MFS families), factor channel-like proteins, linking two, periplasmic adaptor proteins (PAPs), also known as fusion (MFPs). In this review we summarize recent advances understanding biology, regulation these systems, highlighting previously undescribed role PAPs providing a common architectural scaffold across families transporters. Despite being built from limited number basic domains, complexes present staggering variety architectures. While key insights have been derived RND transporter closer inspection operation organization different tripartite reveals unexpected analogies between them, including those formed around MFS- ATP-driven transporters, suggesting that they operate principles. Based on proposing new integrated model PAP-mediated communication within conformational cycling which could be expanded other types assemblies.

Language: Английский

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Effects of emerging pollutants on the occurrence and transfer of antibiotic resistance genes: A review

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 420, P. 126602 - 126602

Published: July 7, 2021

Language: Английский

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