Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(26), P. 11542 - 11553
Published: June 13, 2024
Nanoplastics
(NPs)
are
emerging
pollutants
and
have
been
reported
to
cause
the
disintegration
of
anaerobic
granular
sludge
(AnGS).
However,
mechanism
involved
in
AnGS
was
not
clear.
In
this
study,
polyvinyl
chloride
nanoplastics
(PVC-NPs)
were
chosen
as
target
NPs
their
long-term
impact
on
structure
investigated.
Results
showed
that
increasing
PVC-NPs
concentration
resulted
inhibition
acetoclastic
methanogens,
syntrophic
propionate,
butyrate
degradation,
well
disintegration.
At
presence
50
μg·L
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 31, 2024
Determining
the
taxonomy
and
relative
abundance
of
microorganisms
in
metagenomic
data
is
a
foundational
problem
microbial
ecology.
To
address
limitations
existing
approaches,
we
developed
‘SingleM’,
which
estimates
community
composition
using
conserved
regions
within
universal
marker
genes.
SingleM
accurately
profiles
complex
communities
known
species,
only
tool
that
detects
species
without
genomic
representation,
even
those
representing
novel
phyla.
Given
SingleM’s
computational
efficiency,
applied
it
to
248,559
publicly
available
metagenomes
show
vast
majority
samples
from
marine,
freshwater,
sediment
soil
environments
are
dominated
by
lacking
representation
(median
75.0%).
also
provides
way
identify
for
recovery
metagenome-assembled
genomes
lineages
interest,
can
incorporate
user-recovered
into
its
reference
database
improve
profiling
resolution.
Quantifying
full
diversity
Bacteria
Archaea
shows
genome
databases
far
saturated.
FEMS Microbiology Reviews,
Journal Year:
2021,
Volume and Issue:
46(2)
Published: Dec. 3, 2021
The
mutual
nutritional
cooperation
underpinning
syntrophic
propionate
degradation
provides
a
scant
amount
of
energy
for
the
microorganisms
involved,
so
often
acts
as
bottleneck
in
methanogenic
systems.
Understanding
ecology,
physiology
and
metabolic
capacities
propionate-oxidizing
bacteria
(SPOB)
is
interest
both
engineered
natural
ecosystems,
it
offers
prospects
to
guide
further
development
technologies
biogas
production
biomass-derived
chemicals,
important
forecasting
contributions
by
biogenic
methane
emissions
climate
change.
SPOB
are
distributed
across
different
phyla.
They
can
exhibit
broad
capabilities
addition
syntrophy
(e.g.
fermentative,
sulfidogenic
acetogenic
metabolism)
demonstrate
variations
interplay
with
cooperating
partners,
indicating
nuances
their
lifestyle.
In
this
review,
we
discuss
distinctions
gene
repertoire
organization
methylmalonyl-CoA
pathway,
hydrogenases
formate
dehydrogenases,
emerging
facets
(formate/hydrogen/direct)
electron
transfer
mechanisms.
We
also
use
information
from
cultivations,
thermodynamic
calculations
omic
analyses
basis
identifying
environmental
conditions
governing
oxidation
various
ecosystems.
Overall,
review
improves
basic
applied
understanding
highlights
knowledge
gaps,
hopefully
encouraging
future
research
engineering
on
metabolism
biotechnological
processes.
Bioengineered,
Journal Year:
2022,
Volume and Issue:
13(3), P. 6420 - 6440
Published: Feb. 28, 2022
Biofilm
formation
on
biotic
or
abiotic
surfaces
is
caused
by
microbial
cells
of
a
single
heterogeneous
species.
protects
microbes
from
stressful
environmental
conditions,
toxic
action
chemicals,
and
antimicrobial
substances.
Quorum
sensing
(QS)
the
generation
autoinducers
(AIs)
bacteria
in
biofilm
to
communicate
with
one
other.
QS
responsible
for
growth
biofilm,
synthesis
exopolysaccharides
(EPS),
bioremediation
pollutants.
EPS
used
wastewater
treatment
due
its
three-dimensional
matrix
which
composed
proteins,
polysaccharides,
humic-like
substances,
nucleic
acids.
Autoinducers
mediate
significantly
degradation
Acyl-homoserine
lactone
(AHL)
producing
as
well
quorum
quenching
enzyme
can
effectively
improve
performance
treatment.
Biofilms-based
reactors
their
economic
ecofriendly
nature
are
industrial
wastewaters.
Electrodes
coated
electro-active
(EAB)
obtained
sewage
sludge,
activated
domestic
effluents
getting
popularity
bioremediation.
Microbial
fuel
involved
production
energy
wastewater.
Synthetic
biological
systems
such
genome
editing
CRISPR-Cas
be
advanced
process
through
modification
metabolic
pathways
within
communities.
This
narrative
review
discusses
impacts
regulatory
approaches
formation,
extracellular
polymeric
substance
synthesis,
role
community
pollutants
effluents.
Bioresource Technology,
Journal Year:
2022,
Volume and Issue:
352, P. 127113 - 127113
Published: April 2, 2022
The
addition
of
granular
activated
carbon
(GAC)
enhanced
the
performance
up-flow
anaerobic
sludge
blanket
(UASB)
reactor
treating
blackwater
at
35
°C.
DNA
were
extracted
from
and
biofilms
attached
to
GAC
submitted
for
shotgun
sequencing.
In
addition,
acyl-homoserine
lactones
(AHLs)
quantified.
Diverse
partners
direct
interspecies
electron
transfer
(DIET)
enriched
in
or
biofilm
(GAC-biofilm)
amended
UASB.
Pedosphaera
parvula,
Syntrophus
aciditrophicus
Syntrophorhabus
aromaticivorans
dominant
syntrophs.
analysis
type
IV
pilus
assembly
genes
further
suggested
DIET
may
be
functioned
through
GAC-biofilm,
while
conductive
pili
aggregates.
AHLs
quantification
quorum
sensing
(QS)
related
indicated
higher
QS
activity
population
level
was
induced
by
GAC.
Overall,
work
illustrated
different
patterns,
that
played
an
important
role
controlling
USAB.
The ISME Journal,
Journal Year:
2023,
Volume and Issue:
17(11), P. 1966 - 1978
Published: Sept. 7, 2023
Abstract
Propionate
is
a
key
intermediate
in
anaerobic
digestion
processes
and
often
accumulates
association
with
perturbations,
such
as
elevated
levels
of
ammonia.
Under
conditions,
syntrophic
ammonia-tolerant
microorganisms
play
role
propionate
degradation.
Despite
their
importance,
little
known
about
these
cross-species
interactions.
Here,
we
present
metagenomes
metatranscriptomic
data
for
novel
thermophilic
bacteria
the
partner
methanogens
enriched
propionate-fed
reactors.
A
metagenome
bacterium
which
propose
provisional
name
‘Candidatus
Thermosyntrophopropionicum
ammoniitolerans’
was
recovered,
together
mapping
its
highly
expressed
methylmalonyl-CoA
pathway
Acetate
degraded
by
acetate-oxidising
candidate
bacterium.
Electron
removal
associated
acetate
oxidation
mediated
hydrogen/formate-utilising
Methanoculleus
sp.
Methanothermobacter
sp.,
latter
observed
to
be
critical
efficient
Similar
dependence
on
not
seen
Expression-based
analyses
indicated
use
both
H2
formate
electron
transfer,
including
reciprocation
sulphuric
compounds
microbial
nanotube-mediated
interspecies
Batch
cultivation
demonstrated
degradation
rates
up
0.16
g
L−1
day−1
at
hydrogen
partial
pressure
4–30
Pa
available
energy
around
−20
mol−1
propionate.
These
observations
outline
multiple
interactions
required
represent
first
step
increasing
knowledge
acid
accumulation
high-ammonia
biogas
production
systems.
