Toxicity enhancement of microplastics released from food containers through thermal aging: Absorbing more serum proteins thus activating the innate immune response via actin polymerization

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109358 - 109358

Published: March 1, 2025

This study examined the effects of hot high-fat simulants on physicochemical properties microplastics (MPs) from polypropylene (PP)-, low-density polyethylene (LDPE)-, and polylactic acid (PLA)-based single-use food container (SUFC) leachates those aging their immunomodulatory effectors. Scenario studies have demonstrated that MPs were released these three types SUFCs. LDPE- PLA-based SUFCs also cellulose. Among SUFCs, only PP particles exhibited a new absorption peak at 1725 cm-1, which phenomenon may be attributed to presence unstable tertiary carbon atoms. Subsequently, we investigated removing additive both thermal-aged with polystyrene (PS) carboxyl-modified PS (PS-COOH) polymer backbones as reference materials. The findings indicated PS-COOH induced comparable innate immune responses, exhibiting similar size SUFC percolates. Consequently, selected original MPs, respectively, evaluate immunity. results revealed thata protein corona formed particle types, more adsorption observed particles. complex enhanced phagocytosis RAW264.7 macrophages increased expression pro-inflammatory genes NOS2 TNF-α through an actin polymerization cross-linking mechanism. In this study, how affect responses using model system, emphasizing importance comprehensive safety evaluations MPs.

Language: Английский

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Unraveling the Plastic Pollution in the Aquatic Environment of the Croatian Krk Island

Water, Journal Year: 2025, Volume and Issue: 17(6), P. 785 - 785

Published: March 8, 2025

The assessment of plastic pollution due to microplastics (MPs) and nanoparticles (NPs) was performed for the first time on water samples from four natural sites located near beaches Krk Island, Croatia, namely Melska, Črnika, rt Šilo, Zala. MP NP occurrence investigated collected during December 2022 June 2023, using bulk method followed by filtration stainless-steel sieves digestion process. Factor analysis Spearman’s correlation revealed that percentage fragments correlated well with salinity (ρ = 0.76, p < 0.05) filaments brown MPs 0.80, 0.05). monitoring throughout sampling periods generally showed a decrease in abundance as size increased. presence indicated potential contributions wastewater outlets, particularly household washing machines, either directly residents or tourists abandoned fishing nets. increased concentration NPs over could signify continuous fragmentation degradation biofilm formation their surface. These findings potentially be explained implemented waste measures along coast which 30 October 2024 officially declared zero-waste island.

Language: Английский

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Advancing Tumor Microenvironment Analysis: A Fluorescence Nanosystem for Caspase-1 Monitoring and Synergistic Therapy

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

The lack of precise, real-time analytical tools for monitoring tumor microenvironment changes during treatment hinders advancements in integrated diagnostic and therapeutic platforms. Traditional caspase-3 strategies are limited by their inability to address drug resistance newly discovered apoptotic pathways, leading reduced accuracy practicality. To overcome these limitations, we developed a fluorescence-based "Trojan horse" nanosystem, PFpR@CM, featuring high-sensitivity Caspase-1 detection, tumor-targeted delivery, photothermal therapy. was selected as biomarker due its ability provide accurate feedback on reactive oxygen species (ROS) generation. system employs Fe-doped polydopamine nanoparticles red fluorescent carbon quantum dots (RCQDs) the core, achieving detection limit 0.024 U/mL with linear range 0.05-1.0 U/mL. By integrating MG-63 cell membrane camouflage, PFpR@CM ensures specificity immune evasion, allowing precise situ ROS production ferroptosis. Experimental results demonstrate that enables simultaneous fluorescence tracking localized interventions, over 80% volume reduction vivo minimal systemic toxicity. This work establishes novel chemistry approach multifunctional treatment, providing an innovative solution challenges precision oncology.

Language: Английский

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Oil-Encapsulated Nanoplastics from Plastic Disposable Food Containers Induce Rapid Cell Death through Cell Membrane Disruption

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

The rise of food delivery culture has resulted in millions tons food-contact plastic containers being used each year, yet the risks micro(nano)plastics (MNPs) released during storage oil-rich foods are still not well understood. Here, we investigated MNP release from polypropylene (PP) and polyethylene (PE)-coated under simulated takeout scenarios. When exposed to cooking oil microwave heating, up 125-fold more MNPs 471-fold heavy metals compared water exposure, with significantly altered physicochemical properties. Oil-derived PP NPs, uniquely encapsulated films positive surface charge (+7.37 mV). oil-derived NPs may enhance their interaction negatively charged cell membranes, leading rapid death within 5 minutes at a concentration 100 μg/mL through membrane disruption. Transcriptomic analysis revealed that oil-PP triggered substantially extensive gene expression changes than water-derived particularly pathways related acute cellular stress mitochondrial energy metabolism. Global exposure assessments highlight annual human intake 3.35 g food. Based on integrated molecular endpoints, established benchmark dose lower limit (BMDL) 1.18 for NPs. abundance biological samples exceeds BMDL thresholds, suggesting significant health risks. Our findings reveal previously unknown mechanisms toxicity underscore urgent need stricter regulation packaging foods.

Language: Английский

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Robust Controlled Degradation of Enzyme Loaded PCL‐Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis enzyme-loaded (Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning healing is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation-control technique. The obtained exhibit tunable rates, achieving complete within 12-72-h cycles. acidic products further elucidated reveal potential mechanism. create optimal microenvironment during hemostasis inflammation stages healing. Notably, vivo experiments demonstrate effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, regulate fibroblast differentiation. This promotes rapid a remarkable scarless rate over 99% by day 21. New guidelines for scar-free proposed, which carry out faster without microplastics (MPs) toxic byproducts before principles might provide valuable insights promise developing more effective dressings.

Language: Английский

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Importance of Selenoprotein O in Regulating Hmgb1: A New Direction for Modulating ROS-Dependent NETs Formation to Aggravate the Progression of Acute Liver Inflammation

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 6, 2025

Selenoproteins (Sels) are a class of essential biomolecules that play critical roles in cellular homeostasis. SelO was identified as the preferential source selenium liver, implying its potential key regulatory factor hepatic pathophysiology. Bioinformatics analysis data from GEO sets revealed marked downregulation liver injury. However, function and mechanisms remain unclear. To address this, we investigated effect ablation on acute inflammation, focusing association with inflammation neutrophil extracellular traps (NETs) formation. Wild-type (WT) SelO-knockout mice were used to establish lipopolysaccharide (LPS) exposure model coculture (AML12 cells neutrophils) vitro. Our findings LPS stimulation significantly reduced expression WT mouse liver. deletion promoted Hmgb1 marker cytokines for chemokines, NETs generation, pyroptosis induced an imbalance redox Immunofluorescence, SYTOX staining, scanning electron microscopy confirmed silencing reactive oxygen species (ROS)-dependent Moreover, demonstrated excessive formation exacerbated SelO-ablation-induced inflammation. Importantly, significant involvement Hmgb1/ROS axis development absence SelO. results recruitment enhanced ROS-dependent by increasing levels, thereby aggravating LPS-induced This study not only uncovered crucial biological functions SelO, but also shed light implications inflammatory process.

Language: Английский

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The Overview of the Possible Exposure of Infants to Microplastics

Published: Feb. 1, 2024

Microplastics are small plastic pieces with sizes less than 5 mm. They becoming a global concern due to the potential risk human health. The risks of microplastics may be greater for infants because they do not have sufficiently developed metabolizing enzymes, lower ability remove them, high sensitivity target organs. Infants should breastfed first six months life. Breast milk is considered most complete and suitable source nutrition. However, if breastfeeding during this period possible, it necessary use formulas designed infant initial feeding. exposed higher levels MPs through foods or plastics products. aim study describe possible sources exposure such as placenta, feeding bottles, toys well presence released in feces, breast formulas. There still enough data available from studied area. Therefore, pay increased attention minimizing negative effects on

Language: Английский

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Overview of the environmental risks of microplastics and their controlled degradation from the perspective of free radicals

Environmental Pollution, Journal Year: 2024, Volume and Issue: 355, P. 124227 - 124227

Published: May 24, 2024

Language: Английский

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Microplastics as a Threat to Aquatic Ecosystems and Human Health

Toxics, Journal Year: 2024, Volume and Issue: 12(8), P. 571 - 571

Published: Aug. 5, 2024

The threat posed by microplastics has become one of the world's most serious problems. Recent reports indicate that presence been documented not only in coastal areas and beaches, but also water reservoirs, from which they enter bodies aquatic animals humans. Microplastics can bioaccumulate contaminants lead to damage ecosystems. lack comprehensive data makes it challenging ascertain potential consequences acute chronic exposure, particularly for future generations. It is crucial acknowledge there still a substantial need rapid effective techniques identify microplastic particles precise evaluation. Additionally, implementing legal regulations, limiting plastic production, developing biodegradation methods are promising solutions, implementation could limit spread toxic microplastics.

Language: Английский

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α-amanitin induce inflammatory response by activating ROS/NF-κB-NLRP3 signaling pathway in human hepatoma HepG2 cells

Chemosphere, Journal Year: 2024, Volume and Issue: 364, P. 143157 - 143157

Published: Aug. 23, 2024

Language: Английский

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