Host genetic variation and specialized metabolites from wheat leaves enriches for phyllosphere Pseudomonas spp. with enriched antibiotic resistomes

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Antibiotic resistance in plant-associated microbiomes poses significant risks for agricultural ecosystems and human health. Although accumulating evidence suggests a role plant genotypes shaping their microbiome, almost nothing is known about how the changes of genetic information affect co-evolved microbiome carrying antibiotic genes (ARGs). Here, we selected 16 wheat cultivars experimentally explored impact host variation on phyllosphere ARGs, metabolites. Our results demonstrated that significantly influenced resistomes. Wheat exhibiting high ARGs were linked to elevated Pseudomonas populations, along with increased abundances aeruginosa biofilm formation genes. Further analysis 350 spp. genomes from diverse habitats at global scale revealed nearly all strains possess multiple virulence factor (VFGs), mobile elements (MGEs) genomes, albeit lower nucleotide diversity compared other species. These findings suggested proliferation contributed resistance. We further observed direct links between upregulated leaf metabolite DIMBOA-Glc, spp., enrichment which corroborated by microcosm experiments demonstrating DIMBOA-Glc enhanced relative abundance Overall, alterations metabolites resulting throughout evolution may drive development highly specialized microbial communities capable enriching ARGs. This study enhances our understanding plants actively shape clarifies

Language: Английский

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Co-Exposure to Cyazofamid and Polymyxin E: Variations in Microbial Community and Antibiotic Resistance in the Soil‐Animal-Plant System

Published: Jan. 1, 2025

Human activity is accelerating the emergence of fungal pathogens, prompting substantial efforts to discover novel fungicides. The runoff and spray drift from agricultural fields adversely affect aquatic terrestrial nontarget organisms. However, few studies have examined effects co-contamination by agrochemical fungicides pharmaceutical antibiotics on microorganisms antibiotic resistance genes (ARGs) in soil-animal-plant system. To further explore mechanisms, we investigated individual combined a widely used fungicide (cyazofamid, CZF) last-resort (colistin, polymyxin E, PME) soil-earthworm-tomato Our study revealed that CZF PME exerted synergistic toxicity, significantly reducing earthworm survival inhibiting tomato growth. microbial community structure was perturbed, specific bacteria were enriched. Fungicides had distinct bacterial functional pathways: CZF+PME treatments enhanced compound degradation, whereas promoted biological nitrogen cycling. Moreover, increased abundance insertional plasmid-associated number totalARGs bulk rhizosphere soil. We also linkages between communities resistome. findings provide new insights into potential impacts complex real-life environments, such as soil–animal–plant systems.

Language: Английский

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Molecular sorption binding energy and spatial site occupancy determine the competitive sorption of antibiotic clusters on graphene

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161161 - 161161

Published: March 1, 2025

Language: Английский

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Role of Biochar in Remediation of Soil Pollutants

Published: Jan. 1, 2025

Language: Английский

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Stable isotopes and nanoSIMS single-cell imaging reveals soil plastisphere colonizers able to assimilate sulfamethoxazole

Environmental Pollution, Journal Year: 2024, Volume and Issue: 355, P. 124197 - 124197

Published: May 21, 2024

Language: Английский

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Vertical migration and leaching behavior of antibiotic resistance genes in soil during rainfall: Impact by long-term fertilization

Water Research, Journal Year: 2024, Volume and Issue: 267, P. 122508 - 122508

Published: Sept. 24, 2024

Language: Английский

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Co-exposure to cyazofamid and polymyxin E: variations in microbial community and antibiotic resistance in the soil-animal-plant system

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121160 - 121160

Published: Feb. 1, 2025

Language: Английский

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Agricultural crop rotations control dissemination of antibiotics in soil-earthworm-crop continuums

Geoderma, Journal Year: 2025, Volume and Issue: 455, P. 117234 - 117234

Published: Feb. 23, 2025

Language: Английский

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Fighting Antibiotic Resistance: Insights Into Human Barriers and New Opportunities

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

The public health issue of bacterial multi-resistance to antibiotics has gained awareness among the public, researchers, and pharmaceutical sector. Nevertheless, spread antimicrobial resistance been considerably aggravated by human activities, climate change, subsequent increased release antibiotics, drug-resistant bacteria, antibiotic genes in environment. extensive use for medical veterinary purposes not only induced increasing but also other problems, including negative effects on patient's microbiome. Preventive strategies, new treatment modalities, surveillance are progressively set up. A comprehensive approach is, however, lacking urgently tackling this adverse situation. To address challenge, we discussed here main causes driving pollution environment factors favorable emergence drug resistance. We next propose some key priorities research, prevention, surveillance, education supervise an effective clinical sustainable response.

Language: Английский

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Warming exacerbates the effects of pesticides on the soil collembolan gut microbiome and antibiotic resistome

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 492, P. 138294 - 138294

Published: April 14, 2025

Language: Английский

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