An Unusual ’Gift’ from Humans: Third-Generation Cephalosporin-Resistant Enterobacterales in migratory birds along the East Asian-Australasian Flyway harboring widespread resistant plasmids DOI Creative Commons

Zile Cheng,

Yiwen Chen, Min Li

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109320 - 109320

Published: Feb. 12, 2025

Migratory birds play a pivotal role in the global dissemination of antimicrobial resistance genes (ARGs), with shorebirds relying on coastal wetlands during their long-distance migrations, environments often contaminated and conducive to ARG transmission. However, systematic investigations into (AMR) remain scarce. During spring autumn 2023, we collected 893 throat cloacal swabs from 480 shorebirds, representing 28 species, at Chongming Dongtan, critical stopover along East Asian-Australasian Flyway. Our analysis identified six strains, including four extra-intestinal pathogenic E. coli (ExPEC) two K. pneumoniae, that exhibited third-generation cephalosporins, three ExPEC strains exhibiting significant virulence Galleria mellonella infection assays. We conjugative plasmids: E042113F_p1, carrying blaCMY-2 gene coli, M50_p2, blaKPC-2 hypervirulent pneumoniae plasmid harboring aerobactin system. Bioinformatic experimental analyses confirmed these plasmids could transfer without any fitness cost, remaining stable for least 30 passages. Surprisingly, genomic tracing revealed among similar E042113F_p1 (blaCMY-2), earliest was Chinese swallow 2015, subsequent detections wild Mongolia (2017), Russia (2018), Australia (2019). Notably, E04-CMY-like/M50-KPC-like predominantly originated human sources, underscoring activity cross-species transmission AMR. This human-mediated elements wildlife posed substantial risk amplifying disseminating AMR through long-range migratory bird movements, highlighting urgent need international collaboration under One Health framework. Integrated surveillance, environmental management, stringent antibiotic stewardship are mitigating risks by spreading across ecosystems.

Language: Английский

Comprehensive Analysis and Risk Assessment of Antibiotic Contaminants, Antibiotic-Resistant Bacteria, and Resistance Genes: Patterns, Drivers, and Implications in the Songliao Basin DOI

Anjie Chen,

Tingting Zhang, Fangyuan Cheng

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 361, P. 124852 - 124852

Published: Aug. 30, 2024

Language: Английский

Citations

4
An Unusual ’Gift’ from Humans: Third-Generation Cephalosporin-Resistant Enterobacterales in migratory birds along the East Asian-Australasian Flyway harboring widespread resistant plasmids DOI Creative Commons

Zile Cheng,

Yiwen Chen, Min Li

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109320 - 109320

Published: Feb. 12, 2025

Migratory birds play a pivotal role in the global dissemination of antimicrobial resistance genes (ARGs), with shorebirds relying on coastal wetlands during their long-distance migrations, environments often contaminated and conducive to ARG transmission. However, systematic investigations into (AMR) remain scarce. During spring autumn 2023, we collected 893 throat cloacal swabs from 480 shorebirds, representing 28 species, at Chongming Dongtan, critical stopover along East Asian-Australasian Flyway. Our analysis identified six strains, including four extra-intestinal pathogenic E. coli (ExPEC) two K. pneumoniae, that exhibited third-generation cephalosporins, three ExPEC strains exhibiting significant virulence Galleria mellonella infection assays. We conjugative plasmids: E042113F_p1, carrying blaCMY-2 gene coli, M50_p2, blaKPC-2 hypervirulent pneumoniae plasmid harboring aerobactin system. Bioinformatic experimental analyses confirmed these plasmids could transfer without any fitness cost, remaining stable for least 30 passages. Surprisingly, genomic tracing revealed among similar E042113F_p1 (blaCMY-2), earliest was Chinese swallow 2015, subsequent detections wild Mongolia (2017), Russia (2018), Australia (2019). Notably, E04-CMY-like/M50-KPC-like predominantly originated human sources, underscoring activity cross-species transmission AMR. This human-mediated elements wildlife posed substantial risk amplifying disseminating AMR through long-range migratory bird movements, highlighting urgent need international collaboration under One Health framework. Integrated surveillance, environmental management, stringent antibiotic stewardship are mitigating risks by spreading across ecosystems.

Language: Английский

Citations

0