Polyethylene microplastics promote nucleus pulposus cell senescence by inducing oxidative stress via TLR4/NOX2 axis

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 292, P. 117950 - 117950

Published: Feb. 27, 2025

This study aimed to detect and characterize microplastics in intervertebral disc investigate their effects molecular mechanism on degeneration. We collected tissues from cervical, lumbar, thoracolumbar segments used Raman spectroscopy identify microplastics. Among 80 samples, 47 contained microplastics, with polyethylene being the most prevalent type. To explore of (PE-MPs), we established a mouse model nucleus pulposus cell model. Reactive oxygen species (ROS) levels were assessed via immunofluorescence staining, viability was measured using CCK-8 assay, protein expression related Toll-like receptor 4 (TLR4)/NADPH oxidase 2 (NOX2) axis, oxidative stress, degeneration evaluated through western blotting staining. Results showed that PE-MPs exposure led by inducing stress activating TLR4 / NOX2 which increased senescence cells. These mitigated inhibitors. research highlights existence human tissue unveils novel induced PE-MPs, offering new avenues for clinical treatment microplastic-related

Language: Английский

Acute Effects of Size Dependent Low-Density Polyethylene Microplastics on Zebrafish

Water Air & Soil Pollution, Journal Year: 2025, Volume and Issue: 236(6)

Published: April 12, 2025

Language: Английский

Occurrence, analysis, and toxicity of polyethylene terephthalate microplastics: a review

Environmental Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Language: Английский