Antibiotic resistome in a large urban-lake drinking water source in middle China: Dissemination mechanisms and risk assessment

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 424, P. 127745 - 127745

Published: Nov. 10, 2021

Language: Английский

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Distribution of the microbial community and antibiotic resistance genes in farmland surrounding gold tailings: A metagenomics approach

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 779, P. 146502 - 146502

Published: March 18, 2021

Language: Английский

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A framework for standardized qPCR-targets and protocols for quantifying antibiotic resistance in surface water, recycled water and wastewater

Critical Reviews in Environmental Science and Technology, Journal Year: 2022, Volume and Issue: 52(24), P. 4395 - 4419

Published: Jan. 16, 2022

Water environments are increasingly recognized as a conduit for the spread of antibiotic resistance, but there is need to standardize resistance monitoring protocols ensure comparability across studies. Quantitative polymerase chain reaction (qPCR) attractive sensitive means quantifying genes (ARGs) and has been applied broadly over past two decades various water matrices. QPCR avoids challenges biases associated with culture-based methods, providing reproducible highly measure ARGs carried bacterial community. However, numerous quality assurance other aspects that be addressed measurements representative comparable Here we conducted critical review identify gene targets most commonly measured by qPCR quantify in surface water, recycled wastewater assess corresponding protocols. Identified monitored samples included sul1, tetA, intI1, given their abundance tendency correlate anthropogenic inputs, vanA blaCTX-M, more rarely detected, clinically-relevant targets. We identified 117 peer-reviewed studies meeting search criteria application these assays matrices interest systematically assessed protocols, including sample collection concentration, DNA extraction, primer/probe specificity, amplification conditions, amplicon length, PCR inhibition evaluation, limit detection/quantification. Gene copy numbers reported were further compared assay matrix. Based on this comprehensive recommend assays, standardized workflows, reporting five target genes.

Language: Английский

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Antibiotic Resistance in the Finfish Aquaculture Industry: A Review

Antibiotics, Journal Year: 2022, Volume and Issue: 11(11), P. 1574 - 1574

Published: Nov. 8, 2022

Significant challenges to worldwide sustainable food production continue arise from environmental change and consistent population growth. In order meet increasing demand, fish industries are encouraged maintain high growth densities rely on antibiotic intervention throughout all stages of development. The inappropriate administering antibiotics over time introduces selective pressure, allowing the survival resistant bacterial strains through adaptive pathways involving transferable nucleotide sequences (i.e., plasmids). This is one essential mechanisms resistance development in systems. review article focuses main international regulations governing finfish husbandry summarizes recent data regarding distribution aquaculture chain. second part this examines promising alternative approaches production, farming techniques, vaccination that circumvents excessive use, including new animal welfare measures. Then, we reflect adaptations increasingly interdisciplinary perspectives field their greater alignment with One Health initiative.

Language: Английский

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Antibiotic residues in environment: antimicrobial resistance development, ecological risks, and bioremediation

Environmental Science and Pollution Research, Journal Year: 2021, Volume and Issue: 29(3), P. 3355 - 3371

Published: Nov. 12, 2021

Language: Английский

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