Venlafaxine exposure alters mitochondrial respiration and mitomiR abundance in zebrafish brains DOI Creative Commons
Karyn Robichaud, Leslie M. Bragg, Mark R. Servos

et al.

Environmental Toxicology and Chemistry, Journal Year: 2024, Volume and Issue: 43(7), P. 1569 - 1582

Published: May 2, 2024

Abstract Wastewater treatment plant (WWTP) effluent often releases pharmaceuticals like venlafaxine (a serotonin–norephinephrine reuptake inhibitor antidepressant) to freshwater ecosystems at levels causing adverse metabolic effects on fish. Changes fish metabolism can be regulated by epigenetic mechanisms microRNA (small RNA molecules that regulate mRNA translation), including regulating mitochondrial mRNAs. Nuclear-encoded microRNAs gene expression in mammals, and have predicted We aimed identify whether exposure changed respiration resulted differentially abundant (mitomiRs) zebrafish brains. In vitro of brain homogenate below environmentally relevant concentrations (<1 µg/L) caused a decrease respiration, although this was not driven changes Complex I or II function. To these occur vivo, were exposed 1 µg/L for 0, 1, 6, 12, 24, 96 h. had no significant respiration; however, select mitomiRs (dre-miR-301a-5p, dre-miR-301b-3p, dre-miR-301c-3p) also measured, because they bioinformatically cytochrome c oxidase subunit (COI) abundance. These based (with miR-301c-3p abundance differing during the day miR-301b-3p being lower night), with respect sex time sampled. Overall, results demonstrated but seen after acute vivo exposure. Results may differed allows mitigate through could include mitomiR regulation, only acutely exposed. Environ Toxicol Chem 2024;43:1569–1582. © 2024 The Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Language: Английский

Venlafaxine exposure alters mitochondrial respiration and mitomiR abundance in zebrafish brains DOI Creative Commons
Karyn Robichaud, Leslie M. Bragg, Mark R. Servos

et al.

Environmental Toxicology and Chemistry, Journal Year: 2024, Volume and Issue: 43(7), P. 1569 - 1582

Published: May 2, 2024

Abstract Wastewater treatment plant (WWTP) effluent often releases pharmaceuticals like venlafaxine (a serotonin–norephinephrine reuptake inhibitor antidepressant) to freshwater ecosystems at levels causing adverse metabolic effects on fish. Changes fish metabolism can be regulated by epigenetic mechanisms microRNA (small RNA molecules that regulate mRNA translation), including regulating mitochondrial mRNAs. Nuclear-encoded microRNAs gene expression in mammals, and have predicted We aimed identify whether exposure changed respiration resulted differentially abundant (mitomiRs) zebrafish brains. In vitro of brain homogenate below environmentally relevant concentrations (<1 µg/L) caused a decrease respiration, although this was not driven changes Complex I or II function. To these occur vivo, were exposed 1 µg/L for 0, 1, 6, 12, 24, 96 h. had no significant respiration; however, select mitomiRs (dre-miR-301a-5p, dre-miR-301b-3p, dre-miR-301c-3p) also measured, because they bioinformatically cytochrome c oxidase subunit (COI) abundance. These based (with miR-301c-3p abundance differing during the day miR-301b-3p being lower night), with respect sex time sampled. Overall, results demonstrated but seen after acute vivo exposure. Results may differed allows mitigate through could include mitomiR regulation, only acutely exposed. Environ Toxicol Chem 2024;43:1569–1582. © 2024 The Authors. Environmental Toxicology Chemistry published Wiley Periodicals LLC behalf SETAC.

Language: Английский

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