bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 18, 2024
Antimicrobial
resistance
(AMR)
is
a
global
public
health
problem
particularly
accentuated
in
low-and
middle-income
countries,
largely
due
to
lack
of
access
sanitation
and
hygiene,
awareness
knowledge,
the
inadequacy
molecular
laboratories
for
timely
accurate
surveillance
programs.
This
study
introduces
versatile
detection
toolbox
(C12a)
antibiotic
gene
markers
using
CRISPR/Cas12a
coupled
PCR.
Our
can
detect
less
than
3×10
Infection and Drug Resistance,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 1781 - 1790
Published: May 1, 2024
Abstract:
Carbapenem-resistant
Salmonella
enterica
(S.
enterica)
pose
a
significant
threat
to
public
health,
causing
gastroenteritis
and
invasive
infections.
We
report
the
first
emergence
of
carbapenem-resistant
S.
serovar
London
strain,
A132,
carrying
bla
NDM-5
gene
in
China.
Whole-genome
sequencing
bioinformatics
analysis
assigned
A132
be
ST155,
multidrug-resistant
clone
frequently
reported
The
strain
exhibited
resistance
multiple
antibiotics,
with
20
acquired
antibiotic
genes
(ARGs)
identified,
predominantly
located
on
IncFIB
plasmid
(pA132-1-NDM).
Notably,
was
within
an
IS
26
flanked-class
1
integron-IS
CR1
complex,
comprising
two
genetic
cassettes.
One
cassette
is
class
integron,
which
may
facilitate
transmission
entire
while
other
-containing
CR1-
26-
flanked
cassette,
ARGs.
Genbank
database
search
based
-carrying
identified
similar
context
found
transmissible
IncFIA
plasmids
from
Escherichia
coli
(p91)
Enterobacter
hormaechei
(p388)
shared
host
range,
suggesting
potential
for
cross-species
.
To
our
knowledge,
this
case
ST155
harboring
gene.
Phylogenetic
indicated
close
relationship
between
eight
S
strains
isolated
same
province.
However,
differed
by
four
unique
Given
high
transmissibility
F-type
18
ARGs,
it
imperative
implement
vigilant
surveillance
adopt
appropriate
infection
control
measures
mitigate
health.
Keywords:
NDM-5,
London,
carbapenem
resistance,
IncFIB,
integron
International Journal of Microbiology,
Journal Year:
2024,
Volume and Issue:
2024, P. 1 - 14
Published: May 16, 2024
Pseudomonas
aeruginosa
is
an
opportunistic
pathogen
found
in
a
wide
variety
of
environments,
including
soil,
water,
and
habitats
associated
with
animals,
humans,
plants.
From
One
Health
perspective,
which
recognizes
the
interconnectedness
human,
animal,
environmental
health,
it
important
to
study
virulence
characteristics
antibiotic
susceptibility
bacteria.
In
this
study,
we
compared
properties
resistance
profiles
seven
isolates
collected
from
Gulf
Mexico
those
clinical
strains
P.
aeruginosa.
Our
results
indicate
that
marine
tested
exhibit
similar
properties;
they
expressed
different
factors
were
able
kill
Galleria
mellonella
larvae,
animal
model
commonly
used
analyze
pathogenicity
many
bacteria,
contrast,
showed
higher
than
isolates.
Consistently,
exhibited
prevalence
class
1
integron,
indicator
anthropogenic
impact,
Thus,
our
analyzed
isolated
Mexico,
have
properties,
but
lower
resistance,
hospitals.
Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
283, P. 116774 - 116774
Published: July 25, 2024
The
overuse
of
antimicrobials
in
livestock
farming
has
led
to
the
development
resistant
bacteria
and
spread
antibiotic-resistant
genes
(ARGs)
among
animals.
When
manure
containing
these
antibiotics
is
applied
agricultural
fields,
it
creates
a
selective
pressure
that
promotes
acquisition
ARGs
by
bacteria,
primarily
through
horizontal
gene
transfer.
Most
research
on
focuses
their
role
clinical
antibiotic
resistance
transfer
from
environmental
sources
associated
with
humans,
such
as
Escherichia
coli.
study
investigates
class
1
integrons
27
coli
strains
pig
manure.
It
six
common
(ermB,
cmlA,
floR,
qnrS,
tetA,
TEM)
integron
gene,
assessing
prevalence
samples
three
farms.
found
correlations
anticorrelations
genes,
indicating
predisposition
spreading
certain
ARGs.
Specifically,
cmlA
tetA
were
positively
correlated
each
other
negatively
int1,
suggesting
they
are
not
transferred
via
Int1.
Farm
B
had
highest
int1
counts
higher
abundance
TEM
but
lower
levels
genes.
results
underscore
complexity
predicting
ARG
environments
health
risks
humans
food
chain.
study's
offer
valuable
insights
into
profile
swine
livestock,
potentially
aiding
methods
trace
environment.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 18, 2024
Antimicrobial
resistance
(AMR)
is
a
global
public
health
problem
particularly
accentuated
in
low-and
middle-income
countries,
largely
due
to
lack
of
access
sanitation
and
hygiene,
awareness
knowledge,
the
inadequacy
molecular
laboratories
for
timely
accurate
surveillance
programs.
This
study
introduces
versatile
detection
toolbox
(C12a)
antibiotic
gene
markers
using
CRISPR/Cas12a
coupled
PCR.
Our
can
detect
less
than
3×10
