The
ecological
risks
of
biochar-derived
dissolved
organic
matter
(DOM)
to
soil
invertebrates
at
different
organismal
levels
remains
limited.
This
study
comprehensively
explored
the
DOM
on
earthworm
gut
through
assessments
enzyme
activity
response,
histopathology,
microbiomes,
and
metabolomics.
Results
demonstrated
that
disturbed
digestive
enzymes
in
earthworm,
especially
for
10%
DOM300
groups.
integrated
biomarker
response
v2
(IBRv2)
indicated
perturbation
induced
by
was
both
time-dependent
dose-dependent.
Pathological
observations
revealed
damaged
intestinal
epithelium
lumen
earthworms.
Analysis
16S
rRNA
from
microbiota
showed
a
significant
decrease
genera
(Verminephrobacter,
Bacillus,
Microbacteriaceae)
associated
with
inflammation,
disease,
detoxification
processes.
PICRUSt
predictions
stress
responses
metabolism-related
genes
(encoded
glutathione
transferase,
long-chain
fatty
acid-CoA
ligases,
monosaccharide-transport
ATPase)
treatments.
Furthermore,
caused
abnormality
metabolites,
such
as
glutamate,
fumaric
acid,
pyruvate,
citric
which
were
involved
energy
metabolism,
These
findings
contributed
improve
our
understanding
toxic
mechanism
biochar
multiple
perspectives.
