Metabolomic-Based Assessment of Earthworm (Eisenia fetida) Exposure to Different Petroleum Fractions in Soils

Metabolites, Journal Year: 2025, Volume and Issue: 15(2), P. 97 - 97

Published: Feb. 5, 2025

Background/Objectives: Petroleum contamination in soil exerts toxic effects on earthworms (Eisenia fetida) through non-polar narcotic mechanisms. However, the specific toxicities of individual petroleum components remain insufficiently understood. Methods: This study investigates four components—saturated hydrocarbons, aromatic resins, and asphaltenes—on artificially contaminated soil, utilizing a combination biochemical biomarker analysis metabolomics to uncover underlying molecular Results: The results revealed that hydrocarbons are most fraction, with EC50 concentrations significantly lower than those other fractions. All tested fractions triggered notable metabolic disturbances immune responses after 7 days exposure, as evidenced by significant changes metabolite abundance within critical pathways such arginine synthesis, a-linolenic acid metabolism, pentose phosphate pathway. According KEGG pathway analysis, saturated hydrocarbon induced marked glycerophospholipid proline metabolism pathways, contributing stabilization protein structure membrane integrity. Aromatic disrupted arachidonic pathway, leading increased myotube production enhanced defense TCA cycle riboflavin were altered during exposure colloidal affecting energy cellular respiration. asphaltene fraction impacted glycolysis, accelerating cycling meet stress-induced increases demands. Conclusions: accounted for highest level toxicity among petroleum-contaminated soils. contributions overall should not be ignored, each uniquely affects key biological functions. These findings emphasize importance monitoring perturbations caused non-target organisms earthworms. They also reveal specificity different

Language: Английский

Functional Modification of Ferroferric Oxide Nanoparticle Regulates the Uptake, Oxidative Stress, Tissue Damage, and Metabolic Profiles in Eisenia fetida

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Ferroferric oxide nanoparticles (Fe3O4 NPs) are widely utilized as nanoenabled agrochemicals and soil remediation agents, with functional modification significantly enhancing their stability biocompatibility. However, excessive use of Fe3O4 NPs may pose unassessed ecological risks in soils, particularly concerning the regulatory role two most common surface modifiers polyvinylpyrrolidone (PVP) citric acid (CA) which influence interactions organisms potential toxicity. This study evaluated nanotoxic effects bare (B-Fe3O4 NPs), CA-Fe3O4 NPs, PVP-Fe3O4 on Eisenia fetida ecosystems. After 7 days exposure, B-, CA- decreased weight earthworms, caused oxidative stress tissue damage. Functional showed increased accumulation earthworms while alleviating homeostatic imbalance by accelerating activation related enzymes. Moreover, hyperspectral pathological observations indicated that CA PVP modifications effectively alleviated damage via an improvement NP biocompatibility, dispersion evidenced levels inositol metabolites, has been upregulated more B-Fe3O4 NPs. Significant metabolic disturbances were observed, indicating forced to adjust amino metabolism consume energy detoxify repair work supplements toxic assessment provides crucial insights for optimizing safety through functionalization.

Language: Английский