THE EFFECT OF NANOPLASTICS AND MICROPLASTICS ON LUNG MORPHOLOGY AND PHYSIOLOGY: A SYSTEMATIC REVIEW DOI Creative Commons

Y.V. Rybalchenko

The Medical and Ecological Problems, Journal Year: 2024, Volume and Issue: 28(3), P. 42 - 60

Published: Dec. 30, 2024

Airborne microplastic (NP) and nanoplastic (MP) pollution has emerged as a pressing environmental concern with significant implications for human health. While MPs are present both indoors outdoors, indoor concentrations generally higher due to the abrasion of household materials, furniture, other domestic sources. NPs, when inhaled, accumulate in various lung regions, exerting toxic effects potentially leading respiratory diseases. This systematic review, conducted according PRISMA guidelines, evaluates recent experimental studies on pathogenic impact nanomicroplastics (NMPs) tissues using vivo vitro models. The review included 10 analyzing MP accumulation 90 examining pathogenetic mechanisms exposure. Research data indicates predominance polymeric fibers such polypropylene, polyethylene terephthalate, polytetrafluoroethylene, accounting nearly half structures. NMPs exhibit size- composition-dependent accumulation, smaller positively charged particles showing translocation potential systemic circulation organs. highlights synergistic heavy metals pollutants, interaction pulmonary surfactant, role exacerbating Current analysis underscores growing interest NMP-related health risks identifies knowledge gaps, including need standardization NMP toxicity testing further exploration biological systems. findings emphasize importance mitigating exposure safeguard pave way future research long-term impacts airborne

Language: Английский

Microplastics entry routes into Humans, possible health effects, laws, and policies: A review DOI

Ntegang Venant Atem,

Rogers Wainkwa Chia, Jin‐Yong Lee

et al.

Journal of the geological society of Korea, Journal Year: 2024, Volume and Issue: 60(3), P. 319 - 336

Published: Sept. 1, 2024

Language: Английский

Citations

3
Exposure of Polystyrene Nano- and Microplastics in Increasingly Complex In Vitro Intestinal Cell Models DOI Creative Commons

Kristen A. Marcellus,

David Prescott, Michal Scur

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(4), P. 267 - 267

Published: Feb. 11, 2025

With the rise in global plastic production and presence of waste environment, microplastics are considered an emerging environmental contaminant. Human exposure impact on human health not well studied. Recent studies have observed tissues several noted toxicity vitro vivo mammalian models. We examined polystyrene nano- increasingly complex intestinal cell Using undifferentiated Caco-2 mono-culture model, we assessed particle association, cytotoxicity, clearance/retention, whereas differentiated mono- tri-culture transwell models, membrane integrity translocation. Only 50 nm 500 particles were internalized cells; however, no signs cellular at any concentrations tested. Additionally, had barrier integrity, but able to cross basolateral side, albeit attenuated model that a mucus layer. This study reduced some variability common MNPL testing across various further is needed fully understand potential effects exposure.

Language: Английский

Citations

0
Interaction of Polystyrene Nanoplastics with Biomolecules and Environmental Pollutants: Effects on Human Hepatocytes DOI Open Access
Barbara Mognetti, Claudio Cecone,

Katia Fancello

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2899 - 2899

Published: March 22, 2025

The inevitable exposure of humans to micro/nanoplastics has become a pressing global environmental issue, with growing concerns regarding their impact on health. While the direct effects human health remain largely unknown, increasing attention is being given potential role as carriers pollutants and organic substances. This study investigates toxicity 500 nm polystyrene nanoplastics (NPs) hepatocytes (HepG2) in vitro, both alone combination cadmium (Cd), hazardous heavy metal prevalent pollutant. One-hour 100 µg/mL NPs causes significant increase ROS production (+25% compared control) but cell viability remains unaffected even at concentrations much higher than levels. Interestingly, significantly reduce Cd cytotoxicity LC50 (cell control: 55.4% for 50 µM Cd, 66.9% + 10 NPs, 68.4% NPs). Additionally, do not alter cellular lipid content after short-term (24 h). However, when fatty acids are added medium, appear sequester acids, reducing availability impairing uptake by cells dose-dependent manner. We confirmed Dynamic Light Scattering Scanning Electron Microscopy interaction between free acids. Although exhibited minimal our experimental model, collectively findings suggest that predicting extremely challenging, due specific components biological matrix.

Language: Английский

Citations

0
Mapping micro(nano)plastics in various organ systems: their emerging links to human diseases? DOI
Yating Luo,

Xiuya Xu,

Qin Yin

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118114 - 118114

Published: Dec. 1, 2024

Language: Английский

Citations

2
Deciphering the cytotoxicity of micro- and nanoplastics in Caco-2 cells through meta-analysis and machine learning DOI Creative Commons

Raphaela O G Ferreira,

Rajat Nag, Aoife Gowen

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 362, P. 124971 - 124971

Published: Sept. 16, 2024

Plastic pollution, driven by micro- and nanoplastics (MNPs), poses a major environmental threat, exposing humans through various routes. Despite human colorectal adenocarcinoma Caco-2 cells being used as an in vitro model for studying the intestinal epithelium, uncertainties linger about MNPs harming these factors influencing adverse effects. Addressing this lacuna, our study aimed to elucidate pivotal MNP parameters cytotoxicity cells, employing meta-analysis machine learning techniques quantitative assessment. Initial scrutiny of 95 publications yielded 17 that met inclusion criteria, generating dataset 320 data points. This underwent meticulous stratification based on polymer type, exposure time, size, concentration, biological assays utilised. Subsequent dose-response curve analysis revealed moderate correlations selected subgroups, such (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay time exceeding 24 h, with coefficient determination (R

Language: Английский

Citations

1
Investigating the Impact of Microplastics on Fish Muscle Cell Proliferation and Differentiation: Enhancing Food Safety in Cultivated Meat Production DOI Open Access
Taozhu Sun, Alfonso Timoneda,

Amiti Banavar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 13, 2023

Abstract Cultivated meat, a sustainable alternative to traditional livestock farming, has gained attention for its potential environmental and health benefits. However, concerns about microplastic contamination pose challenges, especially when sourcing cells from marine organisms prone bioaccumulation. Additionally, the pervasive presence of microplastics in laboratory settings, ingredients, during production, increases risk unintentional contamination. This study focused on Atlantic mackerel ( Scomber scombrus ) skeletal muscle cell lines examine effects exposure, represented by fluorescent polyethylene microspheres (10-45 µm) performance including proliferation, viability, gene expression, differentiation processes critical cultivated meat production. The results revealed significant impacts attachment proliferation at concentrations 1 µg/mL, 10 50 µg/mL. Notably, µg/mL concentration exerted most pronounced viability both phases. While indicated that size influence remained unaffected, additional contributing factors require further investigation. These findings underscore necessity thoroughly exploring microplastic-cell interactions ensure food safety safeguard within burgeoning industry.

Language: Английский

Citations

2
Investigation of potential detrimental effects of nano- and microplastics in human endometrial stromal cells DOI Creative Commons
Nara Kim, Joo Hyun Park,

Gee Soo Jung

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract Nanoplastics (NPs) and Microplastics (MPs) have become a global concern in recent years. However, the effects of NPs MPs on female reproductive system not been elucidated, yet. In this study, we aimed to establish proper vitro exposure protocol investigate possible cytotoxic human endometrial cells, using different plastic size concentrations. It was noted that smaller plastics, specifically 100 nm 1 µm MPs, exhibited higher propensity for cellular uptake compared larger counterparts such as 5 MPs. These particles demonstrated ability induce notable morphological changes provoke cell death at concentrations exceeding µg/mL over 24-hour period. Furthermore, confirmed accumulations these nucleus cytoplasm cells confocal microscopy real-time imaging device. A significantly rate internalization observed, manifesting discernible correlation between plastics degree penetration into both cytoplasm. conclusion, is first study demonstrate exposures results dynamic within stromal leading specific Our findings imply potential adverse impacts fertility health, underscoring need further research elucidate exact underlying molecular mechanisms.

Language: Английский

Citations

0
Deciphering the Cytotoxicity of Micro- and Nanoplastics in Caco-2 Cells Through Meta-Analysis and Machine Learning DOI
Rafaela Salgado Ferreira, Rajat Nag, Aoife Gowen

et al.

Published: Jan. 1, 2024

Plastic pollution, driven by micro- and nanoplastics (MNPs), poses a major environmental threat, exposing humans through various routes. Despite Caco-2's common use as an intestinal model, uncertainties linger about MNPs harming these cells the factors influencing adverse effects. Addressing this lacuna, our study aimed to elucidate pivotal MNP parameters cytotoxicity in Caco-2 cells, employing meta-analysis machine learning techniques for quantitative assessment. Initial scrutiny of 95 publications yielded 17 meeting inclusion criteria, generating dataset 320 data points. This underwent meticulous stratification based on polymer type, exposure time, size, concentration, biological assays utilised. Subsequent dose-response curve analysis revealed moderate correlations select subgroups, such MTT assay time exceeding 24 hours, with coefficient determination (R2) values 0.50 (p-value: 0.0065) 0.60 0.0018) respectively. concentrations surpassing 10 µg/mL led diminished viability cells. Notably, we observed challenges navigate multidimensional dataset, prompting adoption methodologies surmount limitation. Leveraging random forest achieved improved predictive performance, R2 0.81 low root mean square error (RMSE) 0.15 test set. delineated size principal drivers cell cytotoxicity, offering nuanced insights into intricate relationship between cytotoxicity. Specifically, partial dependence plot highlighted pattern, featuring initial negativity followed consistent increase, subsequent decline, eventual plateau, underscoring criticality targeted interventions regulatory frameworks addressing microplastic contamination. Our provides crucial health impacts plastic informing policymakers interventions, thus contributing comprehensive understanding its human consequences.

Language: Английский

Citations

0
Investigation of Cell-to-cell Transfer of Polystyrene Microplastics Through Extracellular Vesicle-mediated Communication DOI
Nara Kim, Joo Hyun Park, Inha Lee

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Abstract Plastics have become an integral part of human life, and their production is increasing annually. are broken down into small particles known as microplastics (MPs) with particle size < 5 mm in the environment because a variety factors. MPs prevalent all living organisms exposed to effects. In this study, we investigated whether polystyrene (PS)-MPs were transferred from cell-to-cell via extracellular vesicles (EVs). This study showed that cell-derived EVs could transport plastic particles. addition, using real-time imaging device, confirmed PS-MPs transported by accumulated cells. provides understanding potential effects on suggests directions for future research.

Language: Английский

Citations

0
Characterization and toxicological effects of microplastics in PM2.5 in background sites of the southern coast of China DOI
Jian Sun,

Jiaer Yang,

Steven Sai Hang Ho

et al.

Atmospheric Environment, Journal Year: 2024, Volume and Issue: 337, P. 120779 - 120779

Published: Aug. 24, 2024

Language: Английский

Citations

0