Anti-Stemness and Anti-Proliferative Effects of Cadmium on Bovine Mammary Epithelial Cells DOI Creative Commons
Penggang Liu, Xueli Chen, Yuqing Zhao

et al.

Veterinary Sciences, Journal Year: 2024, Volume and Issue: 12(1), P. 7 - 7

Published: Dec. 29, 2024

Cadmium accumulation in the body can damage a variety of organs and impair their development functions. In present study, we investigated effect cadmium on stemness proliferation normal bovine mammary epithelial cells (BMECs). Normal treated with chloride were assessed for expression stemness-related proteins cell proliferation. Western blotting results found that exposure to different concentrations (0, 1.25, 2.5, 5 μm) 48 h significantly increased Gli1 but unexpectedly decreased downstream stem cell-related including BMI1, SOX2, ALDH. However, also observed treatment μm inhibited mammosphere formation using microscopy. this reduced viability mobility. Flow cytometry detection percentage G0 phase S apoptosis rate. Furthermore, levels caspase-8, caspase-3, PARP cleavage as by western blotting. Our study uncovers previously unrecognized role suggests may affect breast impairing self-renewal inducing apoptosis. Therefore, provides important scientific significance regarding whether heavy metal affects development.

Language: Английский

Maternal exposure to polystyrene nanoplastics during gestation and lactation caused fertility decline in female mouse offspring DOI Creative Commons

Xiu Cheng,

Yue Xue, Houpeng Wang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 289, P. 117632 - 117632

Published: Jan. 1, 2025

The impact of micro/nano plastics (MPs/NPs) on human health is a significant area research. Studies the effects maternal exposure to microplastics (MPs) fertility in offspring have been conducted, but damage caused by nanoplastics (NPs) remains ambiguous. In this study, pregnant Kunming mice were exposed 30 mg/kg/day PS-NPs from 0.5 gestation day (GD) 21 days postpartum (dpp). Increased rates miscarriage and premature delivery observed, as well reduced litter size, indicating potential permanent reproductive injury group. Maternal impaired female offsprings. Decreased primordial increased growing follicles observed ovaries at 1 dpp 7 group, activation follicles. This likely due PS-NPs'induction AKT-FOXO3a signaling pathway downregulating AMPK phosphorylation level enhancing mTOR activity. Furthermore, reduction transzonal projections (TZPs) was noted adult RNA sequencing revealed that TZPs related genes may be linked CAMKIIβ, with corresponding downregulation expression levels. Overall, induced profound enduring functions offspring, raising critical alarms regarding multigenerational toxicity risks associated nanoplastic mammals.

Language: Английский

Citations

0
Dissection of the potential mechanism of polystyrene microplastic exposure on cardiomyocytes DOI
Dahui Xue, Jingnan Huang, Xin Sun

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 973, P. 179048 - 179048

Published: March 18, 2025

Language: Английский

Citations

0
Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1 DOI
Yu Sun, Chengcheng Ji, Yuanmei Zhang

et al.

Journal of Cellular Biochemistry, Journal Year: 2025, Volume and Issue: 126(3)

Published: March 1, 2025

PANoptosis is involved in various pathological processes, but its role acute myocardial infarction (AMI) remains unclear. This study aimed to explore the mechanism by which AMI. GSE172270 was used as an internal test set, and GSE159657 served external validation set identify disease targets for WGCNA performed potential hub genes associated with Then, related among of AMI were screened. Additionally, human cardiomyocytes AC16 mouse HL-1 cultured sugar-free serum-free medium 4 h 12 h, expression key regulating detected qRT-PCR. Finally, mRNA interference overexpression experiments conducted verify mRNAs. A total 45 upregulated 125 downregulated differentially expressed (DEGs) identified data set. 891 In 695 552 DEGs identified. Four AMI-PANoptosis (PRDX1, MMACHC, BLVRB, TXNL1) Through qRT-PCR verification, PRDX1 most specific significant gene. The positive regulator ZPB1 upregulated, while negative TAK1 downregulated. cell death (MLKL, p-MLKL, Caspase-3, Caspase-7, GSDMD) culture. However, reversed these effects. Our demonstrated that a

Language: Английский

Citations

0
The role of pyroptosis in environmental pollutants-induced multisystem toxicities DOI
Rui Xu, Longfei Li,

Yijia Ke

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 372, P. 123632 - 123632

Published: April 10, 2025

Language: Английский

Citations

0
Mechanism of Nano‐Microplastics Exposure‐Induced Myocardial Fibrosis: DKK3‐Mediated Mitophagy Dysfunction and Pyroptosis DOI
Liang Xiong,

Ziyi Xiong,

Juan Hua

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 22, 2025

ABSTRACT Nano‐microplastics (NMPs), as environmental pollutants, are widely present in nature and pose potential threats to biological health. This study aims investigate the mechanisms by which NMPs inhibit mitophagy through suppression of dickkopf‐related protein 3 (DKK3) expression, leading NOD‐like receptor family, pyrin domain containing (NLRP3) inflammasome‐mediated cardiomyocyte pyroptosis promoting myocardial fibrosis. Healthy adult male C57BL/6 mice were administered NMP solution via gavage, their cardiac function was monitored. The results showed that exposure significantly reduced left ventricular ejection fraction (LVEF) fractional shortening (LVFS) increased extent Transcriptome sequencing identified 14 differentially expressed genes (DEGs), including MYL7. Using random forest algorithm functional enrichment analysis, DKK3 a key gene. In Vitro experiments further confirmed downregulate thereby inhibiting pyroptosis. elucidates molecular induce fibrosis provides new theoretical bases targets for diagnosis treatment heart diseases.

Language: Английский

Citations

0
Hydrogen bonding-mediated interaction underlies the enhanced membrane toxicity of chemically transformed polystyrene microplastics by cadmium DOI
Wanqing Zhao, Tong Ye, Jianwen Zhou

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 478, P. 135562 - 135562

Published: Aug. 22, 2024

Language: Английский

Citations

3
Polystyrene nanoplastics mediate skeletal toxicity through oxidative stress and the BMP pathway in zebrafish (Danio rerio) DOI Creative Commons
Wanjing Liu, Min Zeng, Ye Li

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 285, P. 117096 - 117096

Published: Sept. 23, 2024

Language: Английский

Citations

3
Cell Death in Crustacean Immune Defense DOI

Zeyan Chen,

Muhammad Tayyab, Defu Yao

et al.

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

ABSTRACT Cell death mechanisms in crustaceans are a complex interplay of processes essential for maintaining cellular homeostasis and immune defense. Modes cell like apoptosis, necroptosis, necrosis well‐documented crustaceans, serving crucial roles removing damaged or infected cells. Unlike other organisms, likely lack pyroptosis, type programmed associated with innate immunity inflammation, because they do not possess the gasdermin genes this process. Recently, NETosis ferroptosis have emerged as significant pathogen NETosis, involving release DNA fibers antimicrobial proteins, helps trap neutralize pathogens, while ferroptosis, an iron‐dependent form death, contributes to lipid peroxidation responses. Cuproptosis, although yet studied context crustacean immunity, shows potential crosstalk particularly regulation metal ion homeostasis, oxidative stress, metabolism. Understanding these offers promising applications aquaculture, such developing targeted modulators enhancing disease resistance economically important species.

Language: Английский

Citations

2
New mechanistic insights of nanoplastics synergistic cadmium induced overactivation of trypsin: Joint analysis from protein multi-level conformational changes and computational modeling DOI
Xiangxiang Li, Yanzhe Wang, Shaoyang Hu

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 135817 - 135817

Published: Sept. 16, 2024

Language: Английский

Citations

2
Microplastics and nanoplastics in cardiovascular disease—a narrative review with worrying links DOI Creative Commons

Haixiang Zheng,

Gianpaolo Vidili, Gavino Casu

et al.

Frontiers in Toxicology, Journal Year: 2024, Volume and Issue: 6

Published: Oct. 10, 2024

With the widespread use of plastic products and increase in waste, microplastics nanoplastics (MNPs) have become an important issue global environmental pollution. In recent years, increasing number studies shown that MNPs may negative impacts on human health. This review aimed to explore association between cardiovascular disease provide outlook for future research. Research has there be a link exposure disease. Laboratory animals exposed often exhibit abnormalities system, such as increased blood pressure, vascular inflammation, myocardial damage. Epidemiological surveys also revealed people are more likely suffer from diseases, hypertension infarction. Although specific impact mechanism is not fully understood, several possible pathways action, including effects toxic substances interference with endocrine system. summary, health, but further research needed confirm its extent guide relevant public health policies.

Language: Английский

Citations

2