The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176923 - 176923
Published: Oct. 18, 2024
Wastewater-based surveillance is a valuable tool for monitoring pathogen transmission in communities, especially regions where formal systems are limited.
Language: Английский
Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42534 - e42534
Published: Feb. 1, 2025
The timely detection of SARS-CoV-2 is crucial for controlling its spread, especially in areas vulnerable to outbreaks. However, due a lack sustainable and low cost methods, early such outbreaks impacting middle-income countries (LMICs). Leveraging Wastewater-Based Epidemiology (WBE), we examined the dissemination evolution SARS CoV2 virus open drains across urban, suburban densely populated cities selected regions state Maharashtra, third largest India. In period from June 2022 May 2023, 44.89 % RNA were positive RT-qPCR wastewater samples collected regions. Whole genome sequencing revealed 22 distinct lineages, with Omicron variant, followed by XBB dominating, alongside other variants as BF, BQ, CH, BA.2.86, albeit lower frequencies. Wastewater surveillance provided insights into viral transmission, complementing clinical surveillance. Notably, our study detected emerging prior reporting, highlighting potential WBE detection. Findings underscore correlation between population density trend load. This also highlighted significance using low-cost, tool, LMICs, where adequate methods are lacking or difficult deploy accessibility.
Language: Английский
Citations
1
The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 972, P. 179010 - 179010
Published: March 16, 2025
Sewage contamination of freshwater occurs in the form raw waste or as effluent from wastewater treatment plants (WWTP's). While (animal and human) under-functioning WWTP's can introduce live enteric bacteria to systems, most WWTP's, even when operating correctly, do not remove bacterial genetic material treated waste, resulting addition DNA, including antibiotic resistance genes, into water columns sediment systems. In systems with both inputs, then, there will be increased interaction between sewage-associated (untreated sewage) DNA (from untreated effluent). To evaluate this understudied environment, we conducted a three-month field-based study tributary Hudson River (NY, USA) that supplies drinking receives discharges several municipalities. Using culture-based analyses, found sewage influences communities tributary, water-sediment exchanges material. Our results also indicated on waterway serves concentrated source intI1 (antibiotic resistance) which appear collect sediments below outfall along fecal indicator bacteria. work captured environmental impact large rain event perturbed populations matrices, independently outflow. This suggests precipitation events are an important cause for tributaries, runoff surrounding environment being factor.
Language: Английский
Citations
1
SSM - Population Health, Journal Year: 2025, Volume and Issue: unknown, P. 101786 - 101786
Published: March 1, 2025
Population health data from wastewater-based epidemiology (WBE) are being used at unprecedented scales worldwide, yet there is limited focus on how to advance equity in the field. Addressing this gap, we conducted a critical review of published literature PubMed, targeting studies intersection WBE and equity. Of 145 articles assessed full-text screening, identified 68 with considerations. These spanned various spatial biochemical targets, addressing domains such as study design methodologies, ethical social considerations, feasibility implementation monitoring. We summarize synthesize equity-oriented considerations across domains. further propose five key research practice, integrate these into conceptual framework illustrate they apply major steps process conducting WBE. include global inequities access, need prevent potential harms stigma via misuse (inappropriate reporting use for criminal surveillance), importance regulation community engagement, particularly amidst growing privatization WBE, especially United States.
Language: Английский
Citations
0
Published: Sept. 5, 2024
Abstract Wastewater-based surveillance has been proposed as a cost-effective toolset to generate data about public health status by detecting specific biomarkers in wastewater samples, shown during the COVID-19 pandemic. In this work, we report on performance of an extensive, building-by-building platform deployed across 38 locations largest private university system Mexico, spanning 19 32 states. Sampling took place weekly from January 2021 and June 2022. Data 343 sampling sites was clustered campus state evaluated through its correlation with seven-day average daily new cases each cluster. Statistically significant linear correlations (p-values below 0.05) were found 25 campuses 13 Moreover, evaluate effectiveness epidemiologic containment measures taken institution potential representative points for future emergencies Monterrey Metropolitan Area, between viral loads samples be stronger Dulces Nombres, treatment plant city (Pearson coefficient: 0.6456, p-value: 6.36710 −8 ), than study 0.4860, 8.288×10 −5 ). However, when comparing after urban mobility returned pre-pandemic levels, levels both became comparable (0.894 0.865 Nombres).
Language: Английский
Citations
1
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 952, P. 175440 - 175440
Published: Aug. 15, 2024
Diverse enteric pathogens, transmitted through human and animal feces, can cause gastroenteritis. Enteric viruses, such as Aichi virus, specifically genotype A (AiV-A), are emerging pathogens that illnesses even at low doses spreading globally. This research developed a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting the 3CD junction colorimetric loop-mediated isothermal amplification (RT-cLAMP) duplex junctions 2BC of AiV-A genome for rapid sensitive detection this virus in metropolitan regional wastewater samples Queensland, Australia. The performance these assays was evaluated using control materials by analyzing samples. In serially diluted materials, RT-qPCR provided quantifiable data (mean 1.51 log
Language: Английский
Citations
0
medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 10, 2024
Abstract Background Infectious disease surveillance and outbreak investigations have significantly benefited from sewage monitoring as an indicator for pathogen circulation in human populations. The use of accelerated during the COVID-19 pandemic with quantification severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) providing predictions SARS-CoV-2 infections hospital admissions. A comprehensive overview how can further inform local regional public health actions proactively is needed to optimize its future use. By conducting a scoping review, we aim provide reported response pathogens. Methods This review will include peer-reviewed published literature databases MEDLINE, EMBASE Web Science. Literature describing field infectious diseases be considered inclusion. not written English, prior 1 January 2014, systematic reviews, editorials letters editor excluded. Screening against inclusion criteria subsequent data extraction performed by two reviewers. described actions, corresponding sampling methods microbiological analytic tools techniques that applied on samples detecting pathogens also extracted. extracted included combined into narrative synthesis.
Language: Английский
Citations
0
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176923 - 176923
Published: Oct. 18, 2024
Wastewater-based surveillance is a valuable tool for monitoring pathogen transmission in communities, especially regions where formal systems are limited.
Language: Английский
Citations
0