Characterization of Glyphosate Resistance and Degradation Profile of Caballeronia zhejiangensis CEIB S4-3 and Genes Involved in Its Degradation DOI Creative Commons

Manuel Isaac Morales-Olivares,

María Luisa Castrejón-Godínez,

Patricia Mussali-Galante

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 651 - 651

Published: March 13, 2025

Herbicides are the most employed pesticides in agriculture worldwide; among them, glyphosate is successful herbicide molecule history. The extensive use of has been related to environmental pollution and toxic effects on non-target organisms. Effective remediation treatment alternatives must be developed reduce presence its adverse effects. Bioremediation using microorganisms proposed as a feasible alternative for treating pollution; due this, identifying characterizing capable biodegrading key task bioremediation polluted sites by this herbicide. This study characterized resistance profile degradation capacity bacterial strain Caballeronia zhejiangensis CEIB S4-3. According results growth inhibition assays agar plates, C. S4-3 can resist exposure high concentrations glyphosate, up 1600 mg/L glyphosate-based (GBH) formulation, 12,000 analytical-grade molecule. In assay liquid media, resisted all evaluated (25–400 mg/L). After 48 h exposure, GBH caused important (>80%) at between 100 400 mg/L, while inhibitions below 15% tested concentrations. Finally, was degrading 60% supplemented culture media (50 mg/L), when used sole carbon source, twelve hours; moreover, also degrade primary metabolite aminomethylphosphonic acid (AMPA). Genomic analysis revealed genes associated with two reported metabolic pathways degradation, sarcosine AMPA pathways. first report metabolism genus strain. from investigation demonstrate that exhibits significant potential biodegradation, suggesting applicability strategies targeting contaminant.

Language: Английский

Characterization of Glyphosate Resistance and Degradation Profile of Caballeronia zhejiangensis CEIB S4-3 and Genes Involved in Its Degradation DOI Creative Commons

Manuel Isaac Morales-Olivares,

María Luisa Castrejón-Godínez,

Patricia Mussali-Galante

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 651 - 651

Published: March 13, 2025

Herbicides are the most employed pesticides in agriculture worldwide; among them, glyphosate is successful herbicide molecule history. The extensive use of has been related to environmental pollution and toxic effects on non-target organisms. Effective remediation treatment alternatives must be developed reduce presence its adverse effects. Bioremediation using microorganisms proposed as a feasible alternative for treating pollution; due this, identifying characterizing capable biodegrading key task bioremediation polluted sites by this herbicide. This study characterized resistance profile degradation capacity bacterial strain Caballeronia zhejiangensis CEIB S4-3. According results growth inhibition assays agar plates, C. S4-3 can resist exposure high concentrations glyphosate, up 1600 mg/L glyphosate-based (GBH) formulation, 12,000 analytical-grade molecule. In assay liquid media, resisted all evaluated (25–400 mg/L). After 48 h exposure, GBH caused important (>80%) at between 100 400 mg/L, while inhibitions below 15% tested concentrations. Finally, was degrading 60% supplemented culture media (50 mg/L), when used sole carbon source, twelve hours; moreover, also degrade primary metabolite aminomethylphosphonic acid (AMPA). Genomic analysis revealed genes associated with two reported metabolic pathways degradation, sarcosine AMPA pathways. first report metabolism genus strain. from investigation demonstrate that exhibits significant potential biodegradation, suggesting applicability strategies targeting contaminant.

Language: Английский

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