Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 1949 - 1949

Published: July 30, 2023

How bacteria respond at the systems level to both genetic and environmental perturbations imposed same time is one fundamental yet open question in biology. Bioengineering or synthetic biology provides an ideal system for studying such responses, as engineered strains always have changes opposed wildtypes are grown conditions which often change during growth maximal yield of desired products. So, were used address outstanding question. Two Bacillus subtilis (MT1 MT2) created previously overproduction N-acetylglucosamine (GlcNAc), environment with a carbon shift from glucose xylose culture system. We had four groups: (1) wildtype (WT) t1; (2) WT t2; (3) mutant (MT1) (4) MT1 t2. By measuring transcriptomes metabolomes, we found that GlcNAc-producing mutants, particularly MT2, higher than but displayed smaller maximum rate wildtype, despite reaching carrying capacity. Underlying observed growth, pathways leading gene expression associated metabolite concentrations t1 bioenergetics, there was energy supply terms ATP GTP, state metric timepoints. Additionally, most top key precursor metabolites equally abundant either Besides that, prominent feature high consistency between transcriptomics metabolomics revealing response. First, metabolomes revealed PCA clusters groups. Second, important functions enriched by overlapped, amino acid metabolism ABC transport. Strikingly, these overlapped those genes showing (positive negative) correlation metabolites. Furthermore, also KEGG identified using weighted coexpression network analysis. All findings suggest responses simultaneous well coordinated metabolic transcriptional levels: they rely heavily on core does not differ much, while transport important. This serves design guide bioengineering, biology,

Language: Английский