Site-specific antibody masking enables conditional activation with different stimuli DOI Creative Commons
Roberta Lucchi, Maria C. Lucana, Montserrat Escobar-Rosales

et al.

New Biotechnology, Journal Year: 2023, Volume and Issue: 78, P. 76 - 83

Published: Oct. 10, 2023

Antibody therapeutics show great potential to treat a variety of diseases. Often, the dose that can be safely administered is limited by side effects arise from interaction with target outside diseased tissue. Conditionally-active antibodies provide an additional layer selectivity improve safety. Distinct external stimuli or internal cues enable different control strategies and applications. However, current antibody masking have low transferability across stimuli. Here we propose versatile approach conditionally mask derivatives its application single chain variable fragment (scFv) against receptor expressed on cancer stem cells in several tumours. Our strategy relies site-specific conjugation polymer engineered cysteine residue through chemically-synthesised linker cleaved response stimulus. We efficiency depends site size mask. An optimised decreases antigen binding up 20-fold affinity fully recovered upon activation exposure light at 365 nm incubation matrix metalloproteinases overexpressed solid This opens possibility rapidly engineer activatable any

Language: Английский

Polymer Nanocomposites DOI

Niharika Das,

Subhraraj Panda, Dipan Kumar Das

et al.

Advances in chemical and materials engineering book series, Journal Year: 2024, Volume and Issue: unknown, P. 169 - 196

Published: Oct. 22, 2024

Polymer nanocomposites and nanostructured polymers represent a transformative frontier in materials science, merging with nanoscale fillers to enhance mechanical, thermal, barrier properties. This chapter delves into the synthesis, characterization, applications of these advanced materials, highlighting their unique structural attributes multifunctional capabilities. By examining various nano such as carbon nanotubes, graphene, nanoparticles, elucidates role improving polymer matrix Key topics include fabrication techniques, interfacial interactions, impact structuring on material performance. The also explores burgeoning fields aerospace, automotive, electronics, biomedical engineering, emphasizing potential revolutionize product design functionality. aims provide foundational understanding principles progress field polymers.

Language: Английский

Citations

0
Site-specific antibody masking enables conditional activation with different stimuli DOI Creative Commons
Roberta Lucchi, Maria C. Lucana, Montserrat Escobar-Rosales

et al.

New Biotechnology, Journal Year: 2023, Volume and Issue: 78, P. 76 - 83

Published: Oct. 10, 2023

Antibody therapeutics show great potential to treat a variety of diseases. Often, the dose that can be safely administered is limited by side effects arise from interaction with target outside diseased tissue. Conditionally-active antibodies provide an additional layer selectivity improve safety. Distinct external stimuli or internal cues enable different control strategies and applications. However, current antibody masking have low transferability across stimuli. Here we propose versatile approach conditionally mask derivatives its application single chain variable fragment (scFv) against receptor expressed on cancer stem cells in several tumours. Our strategy relies site-specific conjugation polymer engineered cysteine residue through chemically-synthesised linker cleaved response stimulus. We efficiency depends site size mask. An optimised decreases antigen binding up 20-fold affinity fully recovered upon activation exposure light at 365 nm incubation matrix metalloproteinases overexpressed solid This opens possibility rapidly engineer activatable any

Language: Английский

Citations

1