Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

As an interspecies signalling molecule, indole that is also regulating the microbial community quorum sensing (QS) can be indispensable factor to influence performance of anaerobic digestion process. Mainly released by Gram-negative bacteria, impact regulation towards methane production in such system hardly exposed. This research intends analyse affected fluctuations waste sewage sludge (WSS) response QS repression and its analogs: 4-fluoroindole (4-FI), 5-fluoroindole (5-FI), 6-fluoroindole (6-FI), 3- indoleacetic acid (3-IAA), indoleacrylic (3-IARA). Illumina MiSeq platform was utilized delve into active microbes, with ribonucleic (RNA) used as template generate 16S metagenomic library. results, all them inhibit generation even substrates (acetic acid) availability, this phenomenon led slight imbalance Gram-positive/negative bacterial composition inactivation viable core fermenters: Firmicutes, Proteobacteria Chloroflexi. Plus, following compounds, indole, 3-IAA, 6-FI directly disrupted productions both acetoclastic hydrogenotropic methanogenic archaea. Methanosarcina acetivorans C2A a prominent synthesizer characterised terms viability synthesis activity against them. Notably, non-viable presence thus lowering production. From here, affirmation on direct indirect inhibitions archaea derivatives will pave way for valuable future exploration mechanism mapping during methanogenesis.

Language: Английский