Anti-SARS-CoV-2 Activity of Andrographis paniculata (Burm.f.) Nees Extract via Inhibition of Spike-mediated Syncytia Formation in HEK293T Cell Model DOI Creative Commons
Pekik Wiji Prasetyaningrum, Ria Fajarwati Kastian,

Metta Novianti

et al.

HAYATI Journal of Biosciences, Journal Year: 2024, Volume and Issue: 31(5), P. 996 - 1006

Published: June 24, 2024

The resurgence of COVID-19 endemic cases at the end 2023 has underscored need for effective treatments. Some severe are often characterized by formation multinucleated syncytial pneumocytes in lungs. Therefore, our study aimed to explore potential Andrographis paniculata (Burm. f) Nees as an antivirus against SARS-CoV-2, which involves syncitia formation. We utilized non-toxic concentrations A. extract on HEK293T cells determined MTT assay, were 1 μg/ml (cell viability 97.96%) and 10 95.24%) further assays. First, we conducted a pseudovirus cellular entry assay model SARS-CoV-2 infection expressing hACE2/TMPRSS2. co-transfected with plasmids hACE2 TMPRSS2, then infected pseudotyped spike*∆G-GFP rVSV or without extract. internalized would trigger GFP expression reporter cells. Next, performed syncytia transfecting hACE2, spike vectors induce intercellular viral transmission. As results, μg/mL significantly lowered number pseudovirus-infected 54.69% (P = 0.02) spike-mediated 42.39% (P<0.001). In conclusion, results suggested that antiviral activity hindering virus cell-to-cell

Language: Английский

Exploring the Active Constituents of Andrographis paniculata in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII DOI Creative Commons

Heng Xu,

Shengzong Lan,

Simin Lin

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 118 - 118

Published: Jan. 20, 2025

Andrographis paniculata is mainly used to treat skin inflammations, wounds, and infections. In this study, Herba, the aerial part of plant, was proven increase viability UVB-damaged HaCat cells reduce reactive oxygen species levels. The chemical composition Herba extract (AHE) analyzed using UPLC-Q-TOF-MS, diterpene lactones were identified as its primary constituents. Then, fraction prepared exhibited similar effects AHE. AHE, component, representative constituent andrographolide all decreased expression IL-1β, IL-6, CDKN1A. Furthermore, protective AHE active ingredients on epidermal stem investigated. Notably, combined treatment with collagen XVII enhanced cells, increased stemness markers integrin β1 p63, differentiation marker keratin 10. This combination demonstrated significant synergy in maintaining homeostasis, which provides evidences for development skin-protective products.

Language: Английский

Citations

0
Anti-dengue Viral Protein NS5 and Antibacterial Efficacy of Andrographolide from Andrographis paniculata: An In Silico Approach DOI
Kandasamy Selvam, Chinnappan Sudhakar,

Arunagiri Ragu Prasath

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

0
Prunin: An Emerging Anticancer Flavonoid DOI Open Access
Juie Nahushkumar Rana, Sohail Mumtaz

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2678 - 2678

Published: March 16, 2025

Despite the substantial advances in cancer therapies, developing safe and effective treatment methodologies is critical. Natural (plant-derived compounds), such as flavonoids, might be crucial a methodology without toxicity toward healthy tissues. Prunin flavonoid with potential to used biomedical applications. has yet undergo thorough scientific research, its precise molecular mechanisms of action remain largely unexplored. This review summarizes therapeutic prunin for first time, focusing on underlying an anticancer compound. gained significant attention due antioxidant, anti-inflammatory, effects. aims unlock how functions at level exert effects, primarily modulating key cellular pathways. Furthermore, we have discussed prunin’s adjunctive therapy conventional treatments, highlighting ability strengthen responses while decreasing drug resistance. Moreover, discussion probes into innovative delivery methods, particularly nanoformulations, that address bioavailability, solubility, stability limitations optimize application. By providing comprehensive analysis properties, this stimulate further exploration using agent, thereby progressing development targeted, selective, safe, methods.

Language: Английский

Citations

0
Evaluation and efficacy of plant extracts in eradicating medically important mosquitoes: a review DOI
K. Dass

Toxicology and Environmental Health Sciences, Journal Year: 2024, Volume and Issue: 16(4), P. 413 - 458

Published: April 21, 2024

Language: Английский

Citations

2
Terminalia bellirica and Andrographis paniculata dietary supplementation in mitigating heat stress-induced behavioral, metabolic and genetic alterations in broiler chickens DOI Creative Commons

Rabie H. Fayed,

Sara E. Ali, Aya M. Yassin

et al.

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: Sept. 3, 2024

Abstract Background Heat stress (HS) is one of the most significant environmental stressors on poultry production and welfare worldwide. Identification innovative effective solutions necessary. This study evaluated effects phytogenic feed additives (PHY) containing Terminalia bellirica Andrographis paniculata behavioral patterns, hematological biochemical parameters, Oxidative biomarkers, HSP70 , I-FABP2 IL10 TLR4 mTOR genes expression in different organs broiler chickens under chronic HS conditions. A total 208 one-day-old Avian-480 chicks were randomly allocated into four treatments (4 replicate/treatment, 52 birds/treatment): Thermoneutral control treatment (TN, fed basal diet); diet + 1 kg/ton PHY); (HS, PHY). Results The findings indicate that led to a decrease feeding, foraging, walking, comfort behavior while increasing drinking resting behavior, also increased red, white blood cells (RBCs WBCs) counts, heterophile/ lymphocyte (H/L) ratio ( P < 0.05); both mean corpuscular volume (MCV), hemoglobin (MCH) decreased 0.05). In addition, negatively impacted lipid, protein, glucose levels, liver kidney function tests, oxidative biomarkers by malondialdehyde (MDA) levels decreasing reduced glutathion (GSH) activity 0.05 ). caused upregulation duodenal gene expression, downregulation all investigated tissues, hepatic ) compared with TN treatment. Phytogenic effectively mitigated heat stress’s negative impacts broilers via an improvement broilers’ hematological, biochemical, marked tissues showed (except liver) Conclusion have ameliorated HS-induced improved immunity as well gut health chickens.

Language: Английский

Citations

2
Anti-SARS-CoV-2 Activity of Andrographis paniculata (Burm.f.) Nees Extract via Inhibition of Spike-mediated Syncytia Formation in HEK293T Cell Model DOI Creative Commons
Pekik Wiji Prasetyaningrum, Ria Fajarwati Kastian,

Metta Novianti

et al.

HAYATI Journal of Biosciences, Journal Year: 2024, Volume and Issue: 31(5), P. 996 - 1006

Published: June 24, 2024

The resurgence of COVID-19 endemic cases at the end 2023 has underscored need for effective treatments. Some severe are often characterized by formation multinucleated syncytial pneumocytes in lungs. Therefore, our study aimed to explore potential Andrographis paniculata (Burm. f) Nees as an antivirus against SARS-CoV-2, which involves syncitia formation. We utilized non-toxic concentrations A. extract on HEK293T cells determined MTT assay, were 1 μg/ml (cell viability 97.96%) and 10 95.24%) further assays. First, we conducted a pseudovirus cellular entry assay model SARS-CoV-2 infection expressing hACE2/TMPRSS2. co-transfected with plasmids hACE2 TMPRSS2, then infected pseudotyped spike*∆G-GFP rVSV or without extract. internalized would trigger GFP expression reporter cells. Next, performed syncytia transfecting hACE2, spike vectors induce intercellular viral transmission. As results, μg/mL significantly lowered number pseudovirus-infected 54.69% (P = 0.02) spike-mediated 42.39% (P<0.001). In conclusion, results suggested that antiviral activity hindering virus cell-to-cell

Language: Английский

Citations

0