Characteristics and Mechanisms of Simultaneous Quinoline and Ammonium Nitrogen Removal by a Robust Bacterium Pseudomonas stutzeri H3

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 687 - 687

Published: March 19, 2025

The discharge of organic and inorganic nitrogenous pollutants in wastewater leads to eutrophication disrupts the ecological balance. Therefore, pressing need for an effective treatment method has become increasingly evident. A robust bacterium Pseudomonas stutzeri H3 capable simultaneous nitrogen removal was isolated from activated sludge coking system. optimal conditions ammonium quinoline were as follows: C/N ratio 15–20, initial pH 7–8, culture temperature 30 °C, shaking speed 150–300 rpm. At 200 mg/L 100 quinoline, strain achieved above 90% efficiency, exhibiting excellent capabilities. outstanding efficiencies presence different sources further confirmed capability H3. whole genome sequencing metabolic intermediates determination performed elucidate gene function annotations, genes, pathways. findings provide a promising pathway treat wastewater.

Language: Английский

Effects of High Salinity on Nitrogen Removal Efficiency and Microbial Community Structure in a Three-Stage AO System

Water, Journal Year: 2025, Volume and Issue: 17(8), P. 1112 - 1112

Published: April 8, 2025

This study investigated the nitrogen removal performance of a three-stage AO reactor for refractory TN and changes in microbial community structure within activated sludge system under varying sodium chloride concentration conditions. Under an influent 0 g/L with sufficient carbon source, rates Total Nitrogen (TN), Chemical Oxygen Demand (CODcr), Ammonium (NH4+-N) remained stable at 98%, 99.7%, 99.9%, respectively. When increased to 20 g/L, activity AOB was significantly inhibited, efficiency dropping 83%, 89%, 70%, respectively, NAR increasing 91.97%. Analytical results demonstrated that both ammonia-oxidizing bacteria (AOB) nitrite-oxidizing (NOB) exhibited inhibited metabolic activities, NOB experiencing earlier functional impairment. NaCl concentrations ≤ 10 conventional via nitrification–denitrification (ND) dominant. exceeded due accumulation NO2−-N, phyla Planctomycetota Proteobacteria maintained dominance community, while partial nitrification (PN) denitrification pathways gradually replaced ND. Extracellular polymeric substance (EPS) secretion emerged as primary defense mechanism against salinity stress. Experimental findings informed proposed strategies including phased acclimatization salt-tolerance enhancement, EPS production regulation, targeted enrichment consortia, which collectively improved by 18.7–22.3% levels g/L. provides theoretical foundations technical references process optimization hypersaline industrial wastewater treatment systems.

Language: Английский

The microbial electrolysis cell combined with anaerobic digestion for high salinity landfill leachate treatment: Operation parameter optimization, microbial analysis and degradation pathways

Journal of Water Process Engineering, Journal Year: 2024, Volume and Issue: 65, P. 105795 - 105795

Published: July 17, 2024

Language: Английский