Rice Straw-Derived Biochar Mitigates Microcystin-LR-Induced Hepatic Histopathological Injury and Oxidative Damage in Male Zebrafish via the Nrf2 Signaling Pathway DOI Creative Commons
Wang Lin, Fen Hu,

Wansheng Zou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 549 - 549

Published: Dec. 18, 2024

Microcystin-leucine arginine (MC-LR) poses a serious threat to aquatic animals during cyanobacterial blooms. Recently, biochar (BC), derived from rice straw, has emerged as potent adsorbent for eliminating hazardous contaminants water. To assess the joint hepatotoxic effects of environmentally relevant concentrations MC-LR and BC on fish, male adult zebrafish (Danio rerio) were sub-chronically co-exposed varying (0, 1, 5, 25 μg/L) (0 100 in fully factorial experiment. After 30 days exposure, our findings suggested that existence significantly decreased bioavailability liver. Furthermore, histopathological analysis revealed mitigated MC-LR-induced hepatic lesions, which characterized by mild damage, such vacuolization, pyknotic nuclei, swollen mitochondria. Compared groups exposed solely MC-LR, malondialdehyde (MDA) increased catalase (CAT) superoxide dismutase (SOD) noticed mixture groups. Concurrently, significant changes mRNA expression levels Nrf2 pathway genes (cat, sod1, gstr, keap1a, nrf2a, gclc) further proved reduces oxidative damage induced MC-LR. These demonstrate decreases liver, thereby alleviating hepatotoxicity through signaling zebrafish. Our results also imply could serve potentially friendly material mitigating detrimental fish.

Language: Английский

Effectiveness and safety of the use of sodium nitrite in patients with hypertension and pulmonary hypertension: a scoping review DOI
Jéssyca Milene Ribeiro, Aleksander Brandão Santana, Letícia de Oliveira Marusso

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

Citations

0
Integration of Fatty Acid-Targeted Metabolome and Transcriptomics Reveals the Mechanism of Chronic Environmental Microcystin-LR-Induced Hepatic Steatosis DOI
Sisi Yan, Ying Liu, Yin Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Microcystin-LR (MC-LR) is a toxin that causes hepatic steatosis. Our previous study found exposure to 60 μg/L MC-LR for 9 months resulted in liver lipid accumulation, but the underlying mechanisms remain elusive. Herein, first time, fatty acid-targeted metabolome and RNA-seq were combined probe effect mechanism of chronic (12-month) treatment on mice metabolism at environmental-related levels (1, 60, 120 μg/L). It was dose-dependently raised serum levels. The total cholesterol (TC) significantly increased following with 1 (equivalent 0.004 μ/L human). Treatment elevated TC triglyceride (TG) both liver. Serum analysis demonstrated 1, caused significant alterations acid profile. Chronic polyunsaturated acids (PUFAs), including conjugated linoleic eicosapentaenoic acid, which positively correlated or TG led decrease accumulation saturated acids, citramalic pentadecanoic docosanoic negatively These findings suggested mild disruption, while pronounced steatosis mice. Transcriptome revealed environmental regulated expression genes involved phosphatidylinositol 3-kinase (PI3K) complex metabolism. Western blotting RT-qPCR confirmed activated PI3K/AKT/mTOR signaling pathway, downstream fads3 gene participates desaturation upregulated, degradation-related genes, acsl1, acsl4, ehhadh inhibited, transport-related slc27a4 apol7a, promoted. Thus, boosts work indicated limit concentration human drinking water safety needs be discussed. provides evidence profile changes gains new insights into treatment-induced

Language: Английский

Citations

0
Nicotinamide Alleviates Synergistic Impairment of Intestinal Barrier Caused by MC-LR and NaNO2 Coexposure DOI
Xingde Du,

Ruiyang Meng,

Hai‐Lei Wei

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(43), P. 24024 - 24034

Published: Oct. 15, 2024

Microcystin-LR (MC-LR) and nitrites from the environment daily life can be ingested absorbed by humans via digestive tract. However, their combined effects on intestinal health remain unclear. Here, impact of MC-LR sodium nitrite (NaNO2) intestines mice was investigated under actual human exposure conditions. After were exposed to (10, 100 μg/L) NaNO2 (30, 300 mg/L) individual in combination for 6 months, it found that synergistically decreased permeability disrupted physical, chemical, immune, microbial barriers. In coexposure groups, synergistic impairment barrier noted with increasing concentrations or NaNO2, but this adverse effect alleviated nicotinamide supplementation. This study underscores potential risks simultaneous ingestion health. The protective role suggests avenues therapeutic intervention against environmental toxin-induced impairment.

Language: Английский

Citations

1
Rice Straw-Derived Biochar Mitigates Microcystin-LR-Induced Hepatic Histopathological Injury and Oxidative Damage in Male Zebrafish via the Nrf2 Signaling Pathway DOI Creative Commons
Wang Lin, Fen Hu,

Wansheng Zou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 549 - 549

Published: Dec. 18, 2024

Microcystin-leucine arginine (MC-LR) poses a serious threat to aquatic animals during cyanobacterial blooms. Recently, biochar (BC), derived from rice straw, has emerged as potent adsorbent for eliminating hazardous contaminants water. To assess the joint hepatotoxic effects of environmentally relevant concentrations MC-LR and BC on fish, male adult zebrafish (Danio rerio) were sub-chronically co-exposed varying (0, 1, 5, 25 μg/L) (0 100 in fully factorial experiment. After 30 days exposure, our findings suggested that existence significantly decreased bioavailability liver. Furthermore, histopathological analysis revealed mitigated MC-LR-induced hepatic lesions, which characterized by mild damage, such vacuolization, pyknotic nuclei, swollen mitochondria. Compared groups exposed solely MC-LR, malondialdehyde (MDA) increased catalase (CAT) superoxide dismutase (SOD) noticed mixture groups. Concurrently, significant changes mRNA expression levels Nrf2 pathway genes (cat, sod1, gstr, keap1a, nrf2a, gclc) further proved reduces oxidative damage induced MC-LR. These demonstrate decreases liver, thereby alleviating hepatotoxicity through signaling zebrafish. Our results also imply could serve potentially friendly material mitigating detrimental fish.

Language: Английский

Citations

0