Streptococcal infection and its antimicrobial resistance profile associated with bovine mastitis in Ethiopia: a systematic review and meta-analysis

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 12, 2025

Background In Ethiopia, bovine mastitis is a major problem affecting production, welfare, and public health. Streptococcus key pathogen that causes often treated with antimicrobials, which can lead to antimicrobial resistance. Nevertheless, the administration of antimicrobials unintentionally facilitate emergence Thus, this study aimed systematically review estimate pooled prevalence streptococcal infection in along associated resistance profiles, provide comprehensive understanding current situation guide effective treatment bacteria. Methods This systematic was carried out according PRISMA guidelines. To proportion resistance, random effects model utilized R software. The databases used included SCOPUS, PubMed, HINARI, Web Science, Google, Google Scholar. Results Twenty-five articles were meta-analysis. overall spp. 20% (95% CI: 17–23%). Significant heterogeneity observed studies ( I 2 = 87%; p < 0.01). Among regions, highest reported for South Nation, Nationality Peoples Region (SNNPR) at 26%, followed by Amhara (24%), Oromia Addis Abeba (19%), Tigray (15%). isolates found patients clinical (24%). spp., Str. agalactiae had 13%. greatest resistant against penicillin (52%), streptomycin, tetracycline, ampicillin (42, 38, 35%, respectively). According information provided meta-analysis, evidence-based risk management measures should be established prevent control dairy cattle. Monitoring reporting are needed Ethiopia’s different regions. minimize stricter guidelines implemented use cattle, particular focus on reducing use.

Language: Английский

Molecular Characterization, Antibiotic Resistance, and Biofilm Formation of Escherichia coli Isolated from Commercial Broilers from Four Chinese Provinces

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1017 - 1017

Published: April 28, 2025

Escherichia coli (E. coli) represents a significant etiological agent of colibacillosis in poultry, resulting considerable economic losses for the global poultry sector. The present study aimed to determine molecular characterization, antibiotic resistance, and biofilm formation E. strains isolated from diseased broilers four provinces China. A total 200 tissue samples were collected intestine, liver, crop, heart, spleen processed microbiological examination. Molecular detection strains, virulence genes, serotypes was performed using polymerase chain reaction (PCR). Antibiotic susceptibility testing assessed disk diffusion 96-well microtiter plate assays. retrieved 68% (136/200) samples. Most resistant enrofloxacin (56%), followed by cefepime (54%), amoxicillin/clavulanate (52%), streptomycin (50%), ampicillin (48%), clindamycin (47%), kanamycin (41%), polymyxin B (37%), tetracycline (35%), sulfamethoxazole/trimethoprim (33%), ceftazidime (31%), meropenem (4.7%), florfenicol (2.9%). Similarly, tested positive at least one gene specific serotypes. Among these, O145 most prevalent serotype, identified 22 isolates (16.2%), O8 (12.5%), O102 (11.8%), O9 (11.0%). tsh (10.2%) gene. This found that 47.1% biofilm-producing, with 62.5% exhibiting weak production, 29.7% mild 7.8% strong production. 24.2% avian pathogenic due presence five or more specifically tsh, ompA, fimC, iss, fyuA, astA, single strain multiplex PCR. recommends continuous surveillance effective control measures reduce burden coli-related infections poultry.

Language: Английский

Subclinical Mastitis in Small-Holder Dairy Herds of Gansu Province, Northwest China: Prevalence, Bacterial Pathogens, Antimicrobial Susceptibility, and Risk Factor Analysis

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2643 - 2643

Published: Dec. 20, 2024

This cross-sectional study assessed the prevalence, bacterial distribution, antimicrobial susceptibility, and potential risk factors associated with subclinical mastitis (SCM) in small-holder dairy herds Gansu Province, Northwest China. Forty cow farms were randomly selected from eight cities/counties six districts of a total n = 530 lactating cows included this study. SCM prevalence was noted at 38.87% 9.72% quarter levels, respectively, based on California Mastitis Test (CMT). The recovered species as follows: S. agalactiae (36.02%), aureus (19.43%), coagulase-negative staphylococci (CNS) (16.11%), dysgalactiae (12.80%), E. coli (9.00%), uberis (6.64%). All isolated bacteria 100% multi-drug-resistant (MDR) except (87.8% MDR). Antimicrobial susceptibility profiles revealed increased resistance (>85%) these pathogens to penicillin, streptomycin, trimethoprim-sulfamethoxazole, vancomycin, erythromycin. However, showed ampicillin, amoxicillin-sulbactam, ceftazidime, neomycin, kanamycin, spectinomycin, norfloxacin, ciprofloxacin, doxycycline. multivariate regression analysis demonstrated that old age, high parity, late lactation, lesions teats, previous history clinical mastitis, higher milk yield, milking training found be (p < 0.001) developing These findings highlight need for routine surveillance, stewardship, effective preventive strategies mitigate production their possible impacts, i.e., infection, public health.

Language: Английский